- LCK-01 (See other available formats)
- Regulatory Status
- Other Names
- Proto-oncogene tyrosine kinase lck, p56-Lck, T-cell specific protein tyrosine kinase
- Mouse IgG1, κ
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- Product Citations
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Lck is a 56 kD tyrosine protein kinase and a member of the src subfamily that contains both an SH2 and SH3 domain. Alternatively spliced isoforms of Lck have been reported. Lck is a cytoplasmic protein bound to CD4 and CD8 in T lymphocytes that participates in antigen-induced T cell activation through phosphorylation of the T cell receptor zeta chain. Lck is activated upon T cell receptor engagement and is involved in the onset of cell cycle progression induced by interleukin-2. Phosphorylation by Csk downregulates the activity of Lck. In addition to CD4 and CD8, Lck has been reported to interact with PI3K through its SH3 domain and tyrosine phosphorylated KHDRBS1/p70 through its SH2 domain. In addition, Lck has been shown to associate with the SAP transmembrane tyrosine phosphatase. The Lck-01 monoclonal antibody recognizes human Lck and has been shown to be useful for Western blotting, immunoprecipitation, immunocytochemistry, and flow cytometry.Product Details
- Antibody Type
- Host Species
- Peptide corresponding to a.a. 22-36 in human Lck
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
ICC, IP - Verified
FC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration range of 1.0 - 5.0 µg/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Does not cross-react with mouse.
Clone may cross-react with other Src tyrosine kinase family members.
- Application References
- Romagnoli P, et al. 2001. Int. Immunol. 13:305.
- Product Citations
AB_2136337 (BioLegend Cat. No. 628301)
AB_2136336 (BioLegend Cat. No. 628302)
- Belongs to the tyrosine family of kinases, src subfamily. Contains one SH2 domain, 1 SH3 domain. Approximately 56 kD, alternatively spliced isoforms have been reported
Cytoplasmic protein, bound to cytoplasmic CD4 and CD8 in T lymphocytes
- Participates in antigen-induced T cell activation, phosphorylates zeta chain of T cell receptor. Involved in interleukin-2 induced onset of cell cycle progression
- Associates with CD4 and CD8 co-receptors. Binds to PI3K through SH3 interactions, binds to tyrosine phosphorylated KHDRBS1/p70 through SH2 domain. Interacts with SAP transmembrane tyrosine phosphatase
- Cell Type
- T cells
- Biology Area
- Cell Biology, Signal Transduction
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
1. Koga Y, et al. 1986. Eur. J. Immunol. 16:1643.
2. Vogel LB, et al. 1995. Biochem. Biophys. Acta 1264:168.
3. Vogel LB, et al. 1993. Mol. Cell. Biol. 13:7408.
4. Bergaman M, et al. 1992. EMBO J. 11:2919.
- Activated upon T cell receptor engagement. Tyrosine phosphorylation by p50 Csk downregulates catalytic activity
- Gene ID
- 3932 View all products for this Gene ID
- View information about Lck on UniProt.org
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