Purified anti-human TCR Vα24-Jα18 (iNKT cell) Antibody

Pricing & Availability
Clone
6B11 (See other available formats)
Other Names
TCR Vα24-Jα18, TCR Vα24-JαQ, invariant NKT cell, iNKT
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A_6B11_PE_070609.jpg
Human peripheral blood lymphocytes stained with purified 6B11 conjugated to PE and CD3 (UCHT1) Alexa Fluor® 647 (top) or mIgG1, κ (MOPC-21) PE isotype control and CD3 (UCHT1) Alexa Fluor® 647 (bottom)
  • A_6B11_PE_070609.jpg
    Human peripheral blood lymphocytes stained with purified 6B11 conjugated to PE and CD3 (UCHT1) Alexa Fluor® 647 (top) or mIgG1, κ (MOPC-21) PE isotype control and CD3 (UCHT1) Alexa Fluor® 647 (bottom)
  • B_6B11_PE_070609.jpg
Cat # Size Price Quantity Avail. Save
342902 100 µg 84€
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Description

Encoded by the TCR Vα24-Jα18 germline configuration, Vα24-JαQ is expressed on a subset of NKT cells, namely invariant NKT (iNKT). Vα24-JαQ TCR interacts with the glycolipid loaded MHC class 1b molecule CD1d, inducing activation and subsequent cytokine production. iNKT cells have been implicated in immune regulation, tumor surveillance, and host response to pathogens. While iNKT cells occur at low frequency in the blood, assorted chemokines contribute to their tissue homing potential.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Cross-Reactivity: Rhesus, Pigtailed Macaque, Sooty Mangabey
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The 6B11 antibody recognizes the invariant CDR3 region of TCR Vα24-JαQ.

Application References
  1. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
Product Citations
  1. Speir M,et al. 2017. Sci Rep.. 10.1038/s41598-017-14690-5. PubMed
Publication Library
RRID
AB_2229301 (BioLegend Cat. No. 342902)

Antigen Details

Structure
Invariant CDR3 region of TCR Vα24-JαQ
Distribution

Invariant NKT cells

Ligand/Receptor
Glycolipid loaded CD1d
Cell Type
NKT cells
Biology Area
Immunology
Molecular Family
TCRs
Antigen References
  1. Thomas SY, et al. 2003. J. Immunol. 171:2571.
  2. Exley MA, et al. 2008. Eur. J. Immunol. 38:1756.
  3. Montoya CJ, et al. 2007. Immunology. 122:1.
  4. Gansuvd B, et al. 2003. J. Immunol. 171:2904.
Gene ID
28659 View all products for this Gene ID
UniProt
View information about TCR V alpha 24-J alpha 18 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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