Purified anti-human Siglec-8 Antibody

Pricing & Availability
7C9 (See other available formats)
Regulatory Status
Other Names
Sialic acid-binding Ig-like lectin 8 (Siglec-8), Siglec8L, Sialoadhesin family member 2 (SAF2)
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
Human peripheral blood cells stained with anti-human CCR3 Alexa Fluor® 647 and 7C9 PE
  • 7C9_PE_020210
    Human peripheral blood cells stained with anti-human CCR3 Alexa Fluor® 647 and 7C9 PE
Compare all formats
Cat # Size Price Quantity Check Availability Save
347102 100 µg 199€
Check Availability

Need larger quantities of this item?
Request Bulk Quote

Siglec-8 is a lectin specific for 6'-sulfo-sLex and a member of the Ig-superfamily. It is expressed almost exclusively in eosinophils; however, basophils and mast cells can express it to a lower degree. Siglec-8 is a 54 kD transmembranal protein; the extracellular domain has one V-set Ig-like domain and two C2-set domains. The cytoplasmic domain has two immunoreceptor tyrosine-based inhibitor motifs (ITIM) that recruit SH2-family phosphatases after tyrosine phosphorylation. There are reports that siglec-8 inhibits the release of histamine and prostaglandin D2 mediated by the IgEFcR. This molelcule is also involved in the induction of apoptosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Recombinant Siglec-8 fused to human IgG Fc
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References
  1. Floyd H, et al. 2000. J. Biol. Chem. 275:861.
  2. Wen T, et al. 2014. J Immunol. 192:5481. PubMed
Product Citations
  1. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  2. Arakawa S, et al. 2018. Allergol Int. 67S:S41. PubMed
  3. Eldredge LC, et al. 2019. Am J Physiol Lung Cell Mol Physiol. 317:L49. PubMed
  4. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  5. Kano G, et al. 2013. J Allergy Clin Immunol. 132:437. PubMed
  6. Wen T, et al. 2014. J Immunol. 192:5481. PubMed
AB_2239311 (BioLegend Cat. No. 347102)

Antigen Details

A lectin of 54 kD, belonging to the Ig-superfamily; has one V-set Ig-like domain and two C2-set domains; has a single transmembrane domain, and two immunoreceptor tyrosine-based inhibitor motifs (ITIM) in the cytoplasmic domain

Eosinophils, basophils, mast cells

Cell adhesion, signal transduction
Phosphatases containing a SH2 domain
Inhibition of IgE receptor-triggered histamine and prostaglandin D2 release, apoptosis
Cell Type
Basophils, Eosinophils, Mast cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family
Adhesion Molecules, Siglec Molecules
Antigen References

1. Bochner BS, et al. 2009. Clin. Exp. Allergy. 39:317.
2. Hudson SA, et al. 2009. J. Pharmacol. Exp. Ther. 330:608.
3. Nutku E, et al. 2005. Biochem. Biophys. Res. Commun. 336:918.

Gene ID
27181 View all products for this Gene ID
View information about Siglec-8 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human Siglec-8

  • PE anti-human Siglec-8

  • APC anti-human Siglec-8

  • PE/Dazzle™ 594 anti-human Siglec-8

  • PE/Cyanine7 anti-human Siglec-8

  • PerCP/Cyanine5.5 anti-human Siglec-8

  • TotalSeq™-C0199 anti-human Siglec-8

  • PE/Cyanine5 anti-human Siglec-8

  • TotalSeq™-D0199 anti-human Siglec-8

  • Biotin anti-human Siglec-8


Login / Register
Remember me
Forgot your password? Reset password?
Create an Account