Purified anti-human IL-7 Antibody

Pricing & Availability
Clone
BVD10-11C10 (See other available formats)
Other Names
Interleukin-7, Lymphopoietin-1 (LP-1), Pre-B cell growth factor, Thymocyte growth factor
Isotype
Rat IgG2a, κ
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506604 500 µg 200€
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Description

IL-7 is a potent lymphoid cell growth factor which promotes the proliferation of pre-B cells, thymocytes, T cell progenitors, and mature T cells. IL-7 induces formation of LAK cells and CTLs, and can stimulate tumoricidal activity of monocytes and macrophages. IL-7 can induce upregulation of proinflammatory cytokines in myeloid cells. IL-7 is expressed by stromal cells. The BVD10-11C10 monoclonal antibody is specific for human interleukin-7 (IL-7).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli - expressed, recombinant human IL-7
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Detection, ELISPOT Detection

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-7 recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 2-8 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Detection: The biotinylated BVD10-11C10 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified BVD10-40F6 antibody (Cat. No. 501302/501304) as the capture antibody.
Additional reported applications (for the relevant formats) include: The BVD10-11C10 is a non-neutralizing antibody.

Application References
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5.
  2. Abrams J, et al. 1995. Curr. Prot. Immunol. John Wiley and Sons New York.
RRID
AB_2125818 (BioLegend Cat. No. 506604)

Antigen Details

Structure
Cytokine; 20-28 kD (Mammalian)
Bioactivity
Proliferation of pre-/pro-B cells, thymocytes, T cells; formation of LAK cells, CTLs, stimulation of tumoricidal activity monocytes/macrophages; upregulate proinflammatory cytokines in myeloid cells
Cell Sources
Adherent bone marrow stromal cells, thymic stromal cells, spleen, keratinocytes
Receptors
Heterodimer IL-7Rα (CD127); γ-subunit (CD132) in common with IL-2R, IL-4R, IL-13R, IL-15R
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Park L, et al. 1992. Adv. Exp. Med. Biol. 323 125.
3. Melchers F, et al. 1992. Adv. Exp. Med. Biol. 323 111.

Regulation
Downregulated by TGF-β
Gene ID
3574 View all products for this Gene ID
UniProt
View information about IL-7 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

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