- HI30 (See other available formats)
- Regulatory Status
- IV N816
- Other Names
- LCA, T200
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.Product Details
- Human, Cross-Reactivity: Chimpanzee
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
CyTOF®, IHC-P - Verified
WB, IHC-F, ICC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.1 - 10 µg/ml is suggested, if the antibody is not available in a pre-diluted format. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11, and Western blotting3.
It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.
- Application References
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Kishihara K, et al. 1993. Cell 74:143.
- Esser M, et al. 2001. J. Virol. 75:6173. (WB)
- Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
- Nagano M, et al. 2007. Blood 110:151.
- Jiang Q, et al. 2008. Blood 112:2858. PubMed
- Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
- Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
- Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
- Lee J, et al. 2015. J Exp Med. 212:385. PubMed
- Breton G, et al. 2015. J Exp Med. 212:401. PubMed
- Marquardt N, et al. 2015. J Immunol. 6:2467. PubMed
- Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
- Product Citations
AB_314389 (BioLegend Cat. No. 304001)
AB_314390 (BioLegend Cat. No. 304002)
- Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)
Hematopoietic cells, not expressed in circulating erythrocytes or platelets
- TCR and BCR mediated activation
- Galectin-1, CD2, CD3, CD4
- Cell Type
- Hematopoietic stem and progenitors, Mesenchymal Stem Cells
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
- Thomas M. 1989. Annu. Rev. Immunol. 7:339.
- Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
- Gene ID
- 5788 View all products for this Gene ID
- View information about CD45 on UniProt.org
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