Purified anti-human CD20 Antibody

Pricing & Availability
2H7 (See other available formats)
Regulatory Status
IV B201
Other Names
B1, Bp35
Mouse IgG2b, κ
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Product Citations
Human peripheral blood lymphocytes were stained with purified anti-CD20 (clone 2H7) (solid line), or mouse IgG2b, κ (dashed line) followed by anti-mouse IgGs FITC.
  • 2H7_Purified_021805
    Human peripheral blood lymphocytes were stained with purified anti-CD20 (clone 2H7) (solid line), or mouse IgG2b, κ (dashed line) followed by anti-mouse IgGs FITC.
  • 2H7_Purified_IHC-F_Antibody_062317
    Human frozen tonsil section was fixed with 4% paraformaldehyde (PFA) for ten minutes and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml purified anti-human CD20 (clone 2H7) overnight at 4°C, followed by 2.5 µg/ml of anti-mouse IgG (clone Poly4053) Alexa Fluor® 594 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
See high resolution IHC-F data...
Cat # Size Price Quantity Avail. Save
302301 25 µg 28,00€
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302302 100 µg 52,00€
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CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.

Product Details
Technical Data Sheet (pdf)

Product Details

Human, Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus, Squirrel Monkey
Antibody Type
Host Species
Human tonsillar B cells
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested
CyTOF®, IHC-F - Verified
IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The epitope recognized by clone 2H7 has been mapped to the sequence YNCEPANPSEKNSPST which lies in the large extracellular loop of human CD20. Additional reported applications (for the relevant formats) include: immunoprecipitation4 and immunohistochemical staining of acetone-fixed frozen sections5.

Application References
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York.
  4. Polyak MJ, et al. 2002. Blood 99:3256. (IP)
  5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
Product Citations
  1. Murayama M, et al. 2015. Nat Commun. 6: 8483. PubMed
  2. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
  3. Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
  4. Henrick BM, et al. 2021. Cell. . PubMed
  5. Gunawan M, et al. 2017. Sci Rep. . 10.1038/s41598-017-16999-7. PubMed
  6. Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
  7. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  8. Olin A, et al. 2018. Cell. 174:1277. PubMed
  9. Tornack J, et al. 2017. PLoS One. 12:e0169119. PubMed
  10. Cottineau J, et al. 2017. J Clin Invest. 127:1991. PubMed
  11. Bruno TC, et al. 2017. Cancer Immunol Res. 0.831944444. PubMed
  12. Agrawal N, et al. 2018. Front Immunol. 2.053472222. PubMed
  13. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  14. Joas S, et al. 2020. Cell Rep. 30:2261. PubMed
  15. Li Z, et al. 2020. J Clin Lab Anal. 34:e23155. PubMed
  16. Kong R, et al. 2019. Cell. 178:567. PubMed
  17. Gruber CN, et al. 2020. Immunity. 53(3):672-684. PubMed
  18. Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed
  19. Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed
AB_314249 (BioLegend Cat. No. 302301)
AB_314250 (BioLegend Cat. No. 302302)

Antigen Details

Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD

B cell, T cell subsets

B cell activation
Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
Cell Type
B cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Hultin L, et al. 1993. Cytometry 14:196.
2. Tedder T, et al. 1994. Immunol. Today 15:450.

Gene ID
931 View all products for this Gene ID
View information about CD20 on UniProt.org

Related FAQs

What is the difference between two anti human CD20 clones 2H7 and 1412?

For clone 1412: This clone specifically recognizes cytoplasmic domain of CD20 and thus can only be used for intracellular flow cytometry. In this instance you will need to include the fixation and permeabilization steps. Please follow the intracellular flow cytometry staining protocol.

For clone 2H7: This clone is ok for regular surface staining for CD20 and there is no need for any fixation and permeabilization steps.

Our technical protocols can be found here.

Go To Top Version: 3    Revision Date: 10/08/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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