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Purified anti-human CD123 Antibody

Pricing & Availability
Clone
6H6 (See other available formats)
Regulatory Status
RUO
Other Names
IL-3Rα, IL-3 Receptor alpha
Isotype
Mouse IgG1, κ
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Product Citations
publications
6H6
Human peripheral blood lymphocytes stained with 6H6 PE and CD45RO PE/Cy5
  • 6H6
    Human peripheral blood lymphocytes stained with 6H6 PE and CD45RO PE/Cy5
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306002 100 µg 81€
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Description

CD123 is the 70 kD transmembrane α chain of the IL-3 receptor. Alone, CD123 binds IL-3 with low affinity; when CD123 associates with CD131 (common β chain), it binds IL-3 with high affinity. CD123 does not transduce intracellular signals upon binding IL-3 and requires the β chain for this function. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, megakaryocytes, and some B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human IL-3Rα transfected COS cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
IHC-F, IP, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 6H6 does not inhibit IL-3 binding to low- or high-affinity IL-3Rs. Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, and immunohistochemical staining of acetone-fixed frozen sections2 and also paraformaldehyde fixed paraffin embedded tissue7.

Application References
  1. Sun Q, et al. 1996. Blood 87:83. (IP, WB)
  2. Herling M, et al. 2003. Blood 101:5007. (IHC)
  3. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  4. Martin-Gayo E, et al. 2010. Blood 115:5366. PubMed
  5. Chen SC, et al. 2010. Arch Dermatol Res. 302:113. PubMed
  6. Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed
  7. Peduzzi E, et al. 2007. J. Invest. Dermatol. 127:638. (IHC)
Product Citations
  1. Dallari S, et al. 2017. Nat Commun. 8:14830. PubMed
  2. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  3. Rosa TLSA, et al. 2022. Front Med (Lausanne). 9:899998. PubMed
  4. Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
  5. Parackova Z, et al. 2020. Sci Rep. 0.759027778. PubMed
  6. Kremenovic M, et al. 2020. Cancers (Basel). 12:. PubMed
  7. Mann ER, et al. 2020. Sci Immunol. :5. PubMed
  8. Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
  9. Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed
  10. Li Y, et al. 2019. Front Immunol. 0.460416667. PubMed
  11. Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
  12. Syrimi E, et al. 2021. iScience. 24:103215. PubMed
  13. O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed
  14. Chen S, et al. 2010. Arch Dermatol Res. 302:113. PubMed
  15. Olin A, et al. 2018. Cell. 174:1277. PubMed
  16. Tripathi T, et al. 2019. Immunohorizons. 0.201388889. PubMed
  17. Baskar R, et al. 2022. Cell Rep Methods. 2:. PubMed
  18. Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed
  19. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  20. Thrash EM, et al. 2020. STAR Protoc. 1:100055. PubMed
  21. Martin-Gayo E, et al. 2010. Blood. 115:5366. PubMed
  22. Henrick BM, et al. 2021. Cell. . PubMed
  23. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  24. Kato M, et al. 2021. PLoS One. 16:e0252116. PubMed
  25. Liu SQ, et al. 2021. Cancer Immunol Res. 9:34. PubMed
  26. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  27. NULL, et al. 2022. Cell. 185:916. PubMed
  28. Saygin C, et al. 2021. Leukemia. 35:3406. PubMed
  29. Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
  30. Chevrier S, et al. 2018. Cell Syst. 0.675. PubMed
  31. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
RRID
AB_314576 (BioLegend Cat. No. 306002)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70 kD
Distribution

Myeloid precursors, basophils, mast cells, macrophages, dendritic cells, megakaryocytes, subset of lymphocytes

Function
Hematopoietic cell proliferation, differentiation
Ligand/Receptor
IL-3
Cell Type
Basophils, Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, Macrophages, Mast cells, Megakaryocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Miyajima A, et al. 1993. Blood 82:1960.

Gene ID
3563 View all products for this Gene ID
UniProt
View information about CD123 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 08/30/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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