Purified anti-EGFR Phosphorylated (Tyr1068) Antibody

Pricing & Availability
Clone
Poly6563 (See other available formats)
Regulatory Status
RUO
Other Names
Epidermal growth factor receptor, HER1
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly6563_Purified_EGFR_Antibody_WB_071813
Hela cells were starved for 16 hours and treated with (lane 2) or without (lane 1) 100 ng/mL EGF and 1 mM pervanadate for 15 minutes. Cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-EGFR (Tyr1068) antibody. Proteins were visualized using an anti-Rabbit-IgG secondary conjugated to HRP and chemiluminescence detection.
  • Poly6563_Purified_EGFR_Antibody_WB_071813
    Hela cells were starved for 16 hours and treated with (lane 2) or without (lane 1) 100 ng/mL EGF and 1 mM pervanadate for 15 minutes. Cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-EGFR (Tyr1068) antibody. Proteins were visualized using an anti-Rabbit-IgG secondary conjugated to HRP and chemiluminescence detection.
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656302 200 µL 280,00€
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Description

EGFR is a transmembrane glycoprotein that belongs to protein kinase superfamily. It is expressed in tissues of epithelial, mesenchymal and neuronal origin and plays major roles in proliferation, differentiation and development. Binding of the protein to its ligand induces receptor dimerization and tyrosine autophosphorylation. Mutations or over-expression of this gene are associated with certain forms of cancer. Tyrosine 1068 is the primary autophosphorylation site in the C-terminal domain of the receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
KLH conjugated modified synthetic peptide
Formulation
Phosphate-buffered solution/50% glycerol/0.09% sodium azide.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, store undiluted at -20°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5-10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes


This product may contain other non-IgG subtypes.

RRID
AB_2562283 (BioLegend Cat. No. 656302)

Antigen Details

Structure
Four isoforms with 405 to 1210 amino acids, predicted molecular weight 45- 170 kD
Distribution

Cell membrane, nuclear membrane and cellular organelle membrane

Interaction
Interacts with EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Activates RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules.
Biology Area
Angiogenesis, Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Immunology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
CD Molecules, Growth Factors, Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References

1. Alameddine RS, et al. 2013. Curr. Opin. Oncol. 25:313.
2. Downward J, et al. 2003. Anticancer Res. 23:3639.
3. Hrustanovic G, et al. 2013. Cancer Biol. Ther. 14:304.
4. Koland JG, et al. 1990. Biochem. Biophys. Res. Commun. 166:90.
5. Wheeler SE, et al. 2012. Clin. Cancer Res. 18:2278.
6. Yano S, et al. 2003. Anticancer Res. 23:3639.

Gene ID
1956 View all products for this Gene ID
UniProt
View information about EGFR on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 03/17/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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