Purified anti-DYKDDDDK Tag Antibody

Pricing & Availability
Clone
L5 (See other available formats)
Other Names
FLAG tag
Isotype
Rat IgG2a, λ
Ave. Rating
4 reviews
Product Citations
publications
L5_WB_100608
Cell extracts from untransfected 293T cells (lane 1) or 293T cells transfected with a plasmid encoding DYKDDDDK-tagged protein (lane 2), using anti-DYKDDDDK, clone L5.
  • L5_WB_100608
    Cell extracts from untransfected 293T cells (lane 1) or 293T cells transfected with a plasmid encoding DYKDDDDK-tagged protein (lane 2), using anti-DYKDDDDK, clone L5.
Cat # Size Price Quantity Avail. Save
637319 25 µg 60€
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637301 200 µg 172€
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637302 500 µg 260€
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637303 1 mg 324€
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637304 5 mg 532€
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Description

The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins.This unique amino acid sequence allows for specific antibody detection in western blotting, immunoprecipitation, and immunostaining techniques.Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
DYKDDDDK tag epitope
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
DYKDDDDK-tagged mouse Langerin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP, ICC, ELISA, FC, Purification - Reported in literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The L5 clone has been demonstrated to have 2-8 fold better sensitivity in WB than another commonly used antibody clone, M2.

Application References
  1. Park SH, et al. 2008. J Immunol Methods. 331:27.
  2. Moon SH, et al. 2010. J. Biol Chem. 285:12935. PubMed
  3. Sasaki M, et al. 2011. J. Biol Chem. 286:39370. PubMed
  4. Sonder SU, et al. 2012. J Immunol. 188:5906. PubMed
  5. Jiang Y, et al. 2013. Int Immunol. 25:235. PubMed
  6. Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
  7. Toyo-Oak K, et al. 2014. J Neurosci. 34:12168. PubMed
Product Citations
  1. Moon S, et al. 2010. J Biol Chem. 285:12935. PubMed
  2. Sønder S, et al. 2012. J Immunol. 188:5906. PubMed
  3. Jiang Y, et al. 2013. Int Immunol. 25:235. PubMed
  4. Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
  5. Toyo-oka K, et al. 2014. J Neurosci. 34:12168. PubMed
  6. Yin J, et al. 2015. Brain. 138: 2553-2570. PubMed
  7. Ren S, et al. 2016. PLoS One. 11: 0152721. PubMed
  8. Das A, et al. 2016. J Biol Chem. 291: 6096 - 6110. PubMed
  9. Shi Y, et al. 2016. Cell Death Differ. 10.1038/cdd.2016.110. PubMed
  10. Mamedov T, et al. 2017. PLoS One.. 10.1371/journal.pone.0183589. PubMed
  11. Bala Tannan N, et al. 2018. PLoS Genet. 14:e1007153. PubMed
  12. Wroblewska A 2018. Cell. 175:1141. PubMed
  13. DaRosa PA 2018. Molecular cell. 72:753. PubMed
  14. Wang X, et al. 2018. Pathog Dis. 76:. PubMed
  15. Wen XY, et al. 2018. JCI Insight. 3:. PubMed
  16. Mamedov T, et al. 2019. PLoS One. 14:e0213438. PubMed
  17. Stroukov W, et al. 2019. Front Genet. 10:163. PubMed
Publication Library
RRID
AB_2749907 (BioLegend Cat. No. 637319)
AB_1134266 (BioLegend Cat. No. 637301)
AB_1134268 (BioLegend Cat. No. 637302)
AB_1134265 (BioLegend Cat. No. 637303)
AB_1134267 (BioLegend Cat. No. 637304)

Antigen Details

Biology Area
Cell Biology
Antigen References

1. Einhauer A. 2001. J. Biochem. Biophys. Methods. 49:455.
2. Knappik A and Pluckthun A. 1994. Biotechniques. 17:754.

Gene ID
NA
UniProt
View information about DYKDDDDK on UniProt.org

Related FAQs

Does anti tag antibody recognize the tag sequence at N terminal or C terminal or within the fusion protein?
Our antibody can recognize the fusion protein with tag either at N-terminus or C-terminus or within the protein if the tag is exposed.
I am unable to detect FLAG tag with L5 antibody clone by IP and/or western blotting.
Some points to consider here include
  • Sometimes changing tag location (form N-terminus to C-terminus or vice versa) or use tandem FLAG tag (3x) can also increase the chances of tag detection
  • Tagged protein is either not expressed or lowly expressed
  • Always include a positive control to make sure the process of working fine
Go To Top Version: 3    Revision Date: 01/25/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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