Purified anti-Cdk2 Antibody

Pricing & Availability
Clone
AN4.3 (See other available formats)
Other Names
p33(CDK2)
Isotype
Mouse IgG2a
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Product Citations
publications
An4dot3_PURE_Cdk2_Antibody_WB_082018
Total cell lysates (15 µg protein) from Raw264.7 (lane 1) and Jurkat cells (lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 2 µg/mL (1:250 dilution) of purified anti-Cdk2 antibody, clone AN4.3 (upper panel). Proteins were visualized by chemiluminescence detection (Cat. No. 426303) using 1:3000 diluted anti-mouse-IgG secondary antibody conjugated to HRP for purified anti-Cdk2 antibody or 1:10000 diluted Direct-Blot™ HRP anti-β-actin antibody, clone W16197A (lower panel). Lane M: Molecular weight ladder.
  • An4dot3_PURE_Cdk2_Antibody_WB_082018
    Total cell lysates (15 µg protein) from Raw264.7 (lane 1) and Jurkat cells (lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 2 µg/mL (1:250 dilution) of purified anti-Cdk2 antibody, clone AN4.3 (upper panel). Proteins were visualized by chemiluminescence detection (Cat. No. 426303) using 1:3000 diluted anti-mouse-IgG secondary antibody conjugated to HRP for purified anti-Cdk2 antibody or 1:10000 diluted Direct-Blot™ HRP anti-β-actin antibody, clone W16197A (lower panel). Lane M: Molecular weight ladder.
Cat # Size Price Quantity Avail. Save
643901 25 µg 68€
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Description

Cyclin-dependent kinase 2 (Cdk2), also known as cell division protein kinase 2, is an enzyme involved in cell cycle control. Binding of Cdk2 to Cyclin E in G1 phase is required for G1 to S phase transition, while binding of Cdk2 to Cyclin A is required to progress through the S phase. Clone AN4.3 does not recognize other CDKs.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CDK2 recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References
  1. Shi L, et al. 2014. J. Immunol. 192:649. PubMed
Product Citations
  1. Shi L, et al. 2014. J Immunol. 192:649. PubMed
Publication Library
RRID
AB_2276103 (BioLegend Cat. No. 643901)

Antigen Details

Structure
There are two isoforms reported in human with predicted MW of 30 kD, 34 kD, respectively; there are also two isoforms reported in mouse with predicted MW of 34 kD, 39 kD, respectively.
Distribution

Nuclear protein, predicted molecular weight 34 kD.

Function
Involved in the control of the cell cycle
Interaction
Cyclins A, B1, B3, D, or E.
Biology Area
Cell Biology, Cell Cycle/DNA Replication
Antigen References

1. Jeffrey PD, et al. 1995. Nature 376:313

Gene ID
1017 View all products for this Gene ID
UniProt
View information about Cdk2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 08/20/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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