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Purified anti-Cdk2 Antibody

Pricing & Availability
Clone
AN4.3 (See other available formats)
Regulatory Status
RUO
Other Names
p33(CDK2)
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
An4dot3_PURE_Cdk2_Antibody_WB_082018
Total cell lysates (15 µg protein) from Raw264.7 (lane 1) and Jurkat cells (lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 2 µg/mL (1:250 dilution) of purified anti-Cdk2 antibody, clone AN4.3 (upper panel). Proteins were visualized by chemiluminescence detection (Cat. No. 426303) using 1:3000 diluted anti-mouse-IgG secondary antibody conjugated to HRP for purified anti-Cdk2 antibody or 1:10000 diluted Direct-Blot™ HRP anti-β-actin antibody, clone W16197A (lower panel). Lane M: Molecular weight ladder.
  • An4dot3_PURE_Cdk2_Antibody_WB_082018
    Total cell lysates (15 µg protein) from Raw264.7 (lane 1) and Jurkat cells (lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 2 µg/mL (1:250 dilution) of purified anti-Cdk2 antibody, clone AN4.3 (upper panel). Proteins were visualized by chemiluminescence detection (Cat. No. 426303) using 1:3000 diluted anti-mouse-IgG secondary antibody conjugated to HRP for purified anti-Cdk2 antibody or 1:10000 diluted Direct-Blot™ HRP anti-β-actin antibody, clone W16197A (lower panel). Lane M: Molecular weight ladder.
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643901 25 µg 81€
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Description

Cyclin-dependent kinase 2 (Cdk2), also known as cell division protein kinase 2, is an enzyme involved in cell cycle control. Binding of Cdk2 to Cyclin E in G1 phase is required for G1 to S phase transition, while binding of Cdk2 to Cyclin A is required to progress through the S phase. Clone AN4.3 does not recognize other CDKs.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CDK2 recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References
  1. Shi L, et al. 2014. J. Immunol. 192:649. PubMed
Product Citations
  1. Shi L, et al. 2014. J Immunol. 192:649. PubMed
RRID
AB_2276103 (BioLegend Cat. No. 643901)

Antigen Details

Structure
There are two isoforms reported in human with predicted MW of 30 kD, 34 kD, respectively; there are also two isoforms reported in mouse with predicted MW of 34 kD, 39 kD, respectively.
Distribution

Nuclear protein, predicted molecular weight 34 kD.

Function
Involved in the control of the cell cycle
Interaction
Cyclins A, B1, B3, D, or E.
Biology Area
Cell Biology, Cell Cycle/DNA Replication
Antigen References

1. Jeffrey PD, et al. 1995. Nature 376:313

Gene ID
1017 View all products for this Gene ID
UniProt
View information about Cdk2 on UniProt.org
Go To Top Version: 4    Revision Date: 06/14/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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