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Purified anti-β-Amyloid, 17-28 Antibody

Pricing & Availability
Clone
A18104E (See other available formats)
Regulatory Status
RUO
Other Names
AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
a-A18104E_PURE_BetaAmyloid_Antibody_020723
IHC staining of purified anti-β-amyloid, 17-28 (clone A18104E) on formalin-fixed paraffin-embedded human Alzheimer’s brain tissue. Following antigen retrieval using formic acid, the tissue was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C. Ultra-Streptavidin HRP Kit (Cat. No. 929501) was used for detection followed by hematoxylin counterstaining. The image was captured with a 60X objective. Scale bar: 50 μm
  • a-A18104E_PURE_BetaAmyloid_Antibody_020723
    IHC staining of purified anti-β-amyloid, 17-28 (clone A18104E) on formalin-fixed paraffin-embedded human Alzheimer’s brain tissue. Following antigen retrieval using formic acid, the tissue was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C. Ultra-Streptavidin HRP Kit (Cat. No. 929501) was used for detection followed by hematoxylin counterstaining. The image was captured with a 60X objective. Scale bar: 50 μm
  • b-A18104E_PURE_BetaAmyloid_Antibody_2_040323
    BSA-conjugated β-amyloid (Aβ) peptides (25 ng total protein) encompassing the C-terminus, middle, and N-terminus regions were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 μg/mL of purified anti-β-Amyloid, 17-28 (clone A18104E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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804601 25 µg 116€
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804602 100 µg 280€
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Description

Alzheimer’s disease is characterized by the accumulation of aggregated amyloid beta (Aβ) peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor proteins (APP) through sequential proteolytic cleavage of APP by β-secretases and γ-secretases generating diverse Aβ species. Aβ can aggregate to form soluble oligomeric species and insoluble fibrillar or amorphous assemblies. Some forms of the aggregated peptides are toxic to neurons.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant fragment of human β-Amyloid, 17-28
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For use in immunohistochemistry, formic acid antigen retrieval is suggested.

This product is verified in Alzheimer’s disease brain lysate and recombinant human APP751 (Cat. No. 842601) by western blotting.

RRID
AB_2936738 (BioLegend Cat. No. 804601)
AB_2936738 (BioLegend Cat. No. 804602)

Antigen Details

Structure
Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.
Distribution

Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, and muscle.
Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and Golgi apparatus.

Function
The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes, protection against oxidative stress, regulation of cholesterol transport, transcription factor, and as an anti-microbial agent.
Interaction
APBA and APBB family
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Antigen References
  1. Kumar A, et al. 2015. Pharmacol Rep. 67:195-203.
  2. Sadigh-Eteghad S, et al. 2015. Med Princ Pract. 24:1-10.
  3. Hampel H, et al. 2015. Expert Rev Neruother. 15:83-105.
  4. Puig KL, et al. 2013. Exp Gerontol. 48:608-11.
  5. Selkoe DJ, et al. 2016. EMBO Mol Med. 8:595-608.
  6. Walsh DM, et al. 2007. J Neurochem. 101(5):1172-84.
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-amyloid 17-28 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 04/03/2023

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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