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Purified anti-BAF53b Antibody

Pricing & Availability
Clone
N332B/15 (See other available formats)
Regulatory Status
RUO
Other Names
Actin Like 6B, 53 KDa BRG1-Associated Factor B, Actin-Related Protein Baf53b, BRG1-Associated Factor 53B, ArpNalpha, ACTL6, Actin-Like Protein 6B, Actin-Like 6, HArpN Alpha
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
N332B-15_Purified_BAF53b_Antibody_112018
Western blot of purified anti-BAF53b antibody (clone N332B/15). Lane 1: Molecular weight marker; Lane 2: 40 µg of mouse brain nuclear lysate; Lane 3: 40 µg of rat brain nuclear lysate; Lane 4: 40 µg of SH-SY5Y cell lysate. The blot was incubated with 10.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • N332B-15_Purified_BAF53b_Antibody_112018
    Western blot of purified anti-BAF53b antibody (clone N332B/15). Lane 1: Molecular weight marker; Lane 2: 40 µg of mouse brain nuclear lysate; Lane 3: 40 µg of rat brain nuclear lysate; Lane 4: 40 µg of SH-SY5Y cell lysate. The blot was incubated with 10.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
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857401 25 µg 67€
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857402 100 µg 132€
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Description

ACTL6/BAF53 proteins share similar homologies to actin-related proteins and are found within the SWI/SNF chromatin remodeling complexes. ACTL6b/BAF53b protein is part of the neural specific SWI/SNF complex, which play important roles in remodeling mononucleosomes in an ATP-dependent fashion, postmitotic neural development, dendritic growth, memory, and synaptic plasticity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Fusion protein corresponding to amino acids 39-114 of human BAF53b
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2783473 (BioLegend Cat. No. 857401)
AB_2783474 (BioLegend Cat. No. 857402)

Antigen Details

Structure
BAF53b is a 426 amino acid protein with a molecular mass of ~47 kD.
Distribution

Tissue Distribution: Moderately expressed in the brain and testis.
Cellular Distribution: Nucleus and cytosol.

Function
BAF53b is involved in the transcriptional activation / repression of select genes by remodeling mononucleosomes. BAF53b is also a component of the SWI/SNF chromatin remodeling complexes that drive enzymatic activites and chromatin structure alterations by changing the DNA-histone contacts within the nucleosome.
Interaction
BAF53 has been shown to interact with c-Myc.
Biology Area
Cell Biology, Neuroscience, Synaptic Biology, Transcription Factors
Antigen References
  1. Yoo M, et al. 2017. Journal of Neuroscience. 37(13):3686-3697.
  2. Vogel-Ciernia A, et al. 2013. Nat Neurosci. 16(5): 552–561.
  3. Zhan X, et al. 2015. Genesis. 53(7):440-8.
  4. Jeonghyeon Park, et al. 2002. Mol Cell Biol. 22(5): 1307–1316.
Gene ID
51412 View all products for this Gene ID
UniProt
View information about BAF53b on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/21/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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