- Clone
- 15G10 (See other available formats)
- Other Names
- Proteins are acetylated by the reversible transfer of acetyl-CoA to the ε-amino group of lysine
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications

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Western blot analysis of HeLa untreated (lane 1) and HeLa starved overnight and treated with sodium butyrate overnight (lane 2) using anti-Acetylated Lysine (clone 15G10) antibody. Purified anti-GAPDH (clone Poly6314) antibody was used as a loading control. -
Untreated HeLa cells (upper panel), or overnight nocodazole treated HeLa cells (lower panel) stained with purified mouse monoclonal antibody against Acetylated Lysine (clone 15G10), followed by Rhodamine Red-X conjugated Donkey anti-mouse IgG and DAPI.
Cat # | Size | Price | Quantity Avail. | Save | ||
---|---|---|---|---|---|---|
623401 | 25 µg | 68€ | ||||
623402 | 100 µg | 148€ |
Proteins are reversibly and dynamically acetylated on the ε-amino group of lysine by acetyltransferases and deacetylated by deacetylases. This post-translational modification can regulate protein function (interactions with other proteins and DNA binding). Histones and transcription factors (PCAF, p53, p300, etc) appear to be the major targets of acetyltransferases. Acetylation is usually associated with chromatin remodeling and transcriptional activation, although in some cases (telomeres) it is associated with gene silencing. The 15G10 antibody recognizes acetylated lysine residues on proteins.
Product DetailsProduct Details
- Reactivity
- Acetylated lysine residues
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Acetylated protein mixture
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store between 2°C and 8°C.
- Application
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WB - Quality tested
ICC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. For immunocytochemistry: Use a dilution range of 1-4 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application References
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- Kuo HP, et al. 2010. Sci Signal. 3:Ra9. PubMed
- Product Citations
- RRID
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AB_315967 (BioLegend Cat. No. 623401)
AB_315968 (BioLegend Cat. No. 623402)
Antigen Details
- Structure
- Proteins are acetylated by the reversible transfer of acetyl-CoA to the ε-amino group of lysine
- Function
- Post-translational modification of proteins by acetyltransferases and deacetylated by deacetylases to regulate protein-protein and protein-DNA interactions
- Biology Area
- Cell Biology, Chromatin Remodeling/Epigenetics
- Antigen References
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1. Wang S, et al. 2004. Novartis Found. Symp. 259:238.
2. Legube G, et al. 2003. EMBO Rep. 4:944.
3. Chen LF, et al. 2003. J. Mol. Med. 81:549. - Gene ID
- 8850 View all products for this Gene ID
- UniProt
- View information about Acetylated Lysine on UniProt.org
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