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Western blot of purified anti-Pan-Shank antibody (clone N23B/49). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 5 µg/mL of N23B/49 or mouse IgG1, κ overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-Pan-Shank antibody (clone N23B/49) on formalin-fixed paraffin-embedded rat cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-MAGUK (pan reactive) antibody (clone K28/86) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin HRP kit (Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
Western blot of purified anti-PSD-93 (Chapsyn-110) antibody (clone N18/28). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system. -
Western blot of purified anti-SAP102 antibody (clone N19/2). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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899911 | 1 kit | 268€ |
The Postsynaptic Antibody Sampler Kit offers flexibility for sampling and detection of major targets localized to the postsynaptic area. These antibodies are highly suitable for WB and IHC applications.
Product DetailsKit Contents
- Kit Contents
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Specificity Format Clone Size Reactivity Isotype Anti-Pan-Shank Purified N23B/49 25 μg Human, Mouse, Rat Mouse IgG1, κ Anti-MAGUK (pan reactive) Purified K28/86 25 μl Human, Mouse, Rat Mouse IgG1 Anti-PSD95 Purified K28/74 25 μl Human, Mouse, Rat Mouse IgG1 Anti-PSD-93 (Chapsyn-110) Purified N18/28 25 μl Human, Mouse, Rat Mouse IgG2a Anti-SAP102 Purified N19/2 25 μl Human, Mouse, Rat Mouse IgG1 * For detailed information about each specificity, please refer to the datasheets of the individual products.
Product Details
- Formulation
- Please refer to individual product datasheets of the purified formats for details.
- Preparation
- All antibodies in this kit were purified by affinity chromatography.
- Storage & Handling
- Upon receipt, store undiluted at 2-8°C.
- Application
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WB, IHC-P - Quality tested
- Recommended Usage
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Each lot of antibodies in this kit is quality control tested by Western blotting. For Western blotting, the suggested uses of these reagents are as follows:
Anti-Pan-Shank: 1.0 - 5.0 µg/ml
Anti-PSD95: 0.1 - 5.0 µg/ml
Anti-PSD-93 (Chapsyn-110): 1.0 µg/ml
Anti-SAP102: 10 µg/ml
For immunohistochemistry on formalin-fixed paraffin-embedded tissue, the suggested use of this reagent is as follows:
Anti-MAGUK (pan reactive): 0.5 - 1.0 µg/ml
It is recommended that the reagent be titrated for optimal performance for each application. - Application Notes
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For verified or reported applications for these antibodies, please see individual product datasheets.
Antigen Details
- Biology Area
- Cell Biology, Neuroscience, Synaptic Biology
- Molecular Family
- Postsynaptic proteins
- Gene ID
- 1742 View all products for this Gene ID 64053 View all products for this Gene ID 58948 View all products for this Gene ID 50944 View all products for this Gene ID 22941 View all products for this Gene ID 85358 View all products for this Gene ID
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