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PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
MOPC-21 (See other available formats)
Isotype
Mouse IgG1, κ
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Product Citations
publications
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
400149 25 tests 64€
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400150 100 tests 164€
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Description

The MOPC-21 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG1, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The immunoglobulin was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The IgG1, κ isotype control solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested
Recommended Usage

Each lot of this mouse IgG1, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA)

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References
  1. Carlsten M, et al. 2007. Cancer Res. 67:1317. PubMed
  2. Smed-Sörensen A, et al. 2008. Blood 111:5037. PubMed (FA)
  3. Bunesmann MM, et al. 2011. Am. J. Respir. Cell. Mol. Biol. Epub. PubMed
  4. Matsuyama T, et al. 2005. Infect. Immun. 73:1044. (IF)
  5. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
  6. Lian IA, et al. 2011. Placenta. 32:823. PubMed
  7. Bufe B, et al. 20015. J Biol Chem. 290:7369. PubMed
Product Citations
  1. Kuen J, et al. 2017. PLoS One. 10.1371/journal.pone.0182039. PubMed
  2. McCauley KB, et al. 2018. Curr Protoc Stem Cell Biol. 45:e51. PubMed
  3. Murata K, et al. 2019. Oncol Lett. 17:5830. PubMed
  4. Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed
  5. Yang C, et al. 2019. J Transl Med. 1.004166667. PubMed
  6. Queckborner S, et al. 2020. Stem Cell Res Ther. 11:15. PubMed
  7. Mendoza-Cabrera MI, et al. 2019. Innate Immun. :1753425919864658. PubMed
  8. Liang Y, et al. 2019. J Allergy Clin Immunol. 144:1025. PubMed
  9. Jacob A, et al. 2020. Nature Protocols. 14(12):3303-3332.. PubMed
  10. Grandclaudon M, et al. 2019. Cell. 179:432. PubMed
  11. Zhu Y, et al. 2012. PLoS One. 7:e44131. PubMed
  12. Diessner J, et al. 2014. Cell Death Dis. 5:1149. PubMed

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 02/22/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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