- JES5-16E3 (See other available formats)
- Regulatory Status
- Other Names
- Interleukin-10, Cytokine synthesis inhibitory factor (CSIF), B cell derived T cell growth factor (B-TCGF)
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
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IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. IL-10 inhibits IFN-γ, TNF-β, and IL-2 production by Th1 clones; inhibits macrophage-mediated IL-1, IL-6, and TNF-α synthesis; suppresses the delayed type hypersensitivity response; stimulates Th2 cell response (which results in elevated antibody production); and promotes mast cell proliferation in combination with IL-4.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- E. coli-expressed, recombinant mouse IL-10
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
ELISA or ELISPOT Detection1,9,11: The biotinylated JES5-16E3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified JES5-2A5 antibody (Cat. Nos. 504902 & 504904) as the capture antibody.
ELISA Capture: The purified JES5-16E3 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated JES5-2A5 antibody (Cat. No. 505003) as the detection antibody and recombinant mouse IL-10 (Cat. No. 575809) as the standard.
Neutralization14: The Ultra-LEAF™ purified JES5-16E3 antibody can neutralize the bioactivity of natural or recombinant IL-10.
Flow Cytometry3: The fluorochrome-labeled JES5-16E3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-10-producing cells within mixed cell populations.
Additional reported applications (for relevant formats) include: immunohistochemistry3.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
- Simkin G, et al. 2000. J. Immunol. 164:2457.
- Kitagaki K, et al. 2002. Clin. Diagn. Lab Immunol. 9:1260.
- Khanna A, et al. 2000. J. Immunol. 164:1346.
- Sander B, et al. 1993. J. Immunol. Methods 166:201.
- Litton M, et al. 1994. J. Immunol. Methods 175:47.
- Andersson U, et al. 1999. Detection and qunatification of gene expression. New York:Springer-Verlag.
- Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons New York. Unit 6.28.
- Wang W, et al. 2004. FASEB J. 18:1043.
- Brummel R and Lenert P. 2005. J. Immunol. 174:2429.
- Lawson BR, et al. 2007. J. Immunol. 178:5366.
- Xu G, et al. 2007. J. Immunol. 179:5358. PubMed
- Brummel R, et al. 2005. J. Immunol.174:2429. PubMed
- Kang YJ, et al. 2007. Stem Cells 25:1814. PubMed
- Seo N, et al. 2001. Immunology. 103:449. (Neut)
- Product Citations
AB_2561522 (BioLegend Cat. No. 505027)
AB_2561523 (BioLegend Cat. No. 505028)
- Acid-labile cytokine, dimer, 17-21 kD (Mammalian)
- Cell Sources
- Activated CD8+ T cells, Th0, Th2 subset of CD4+ T cells, Ly-1+ B cells, monocytes, macrophages, keratinocytes
- Cell Targets
- T cells, B cells, mast cells, macrophages
- IL-10R (CDw210)
- Cell Type
- Biology Area
- Molecular Family
- Antigen References
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. de Waal-Malefy R, et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard M, et al. 1992. Immunol. Today 13:198.
4. Quesniaux V. 1992. Res. Immunol. 143:385.
5. Norton SK, et al. 2008. J. Immunol. 180:2848.
- Downregulated by IL-4, IL-10
- Gene ID
- 16153 View all products for this Gene ID
- View information about IL-10 on UniProt.org
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
|APC anti-mouse IL-10||JES5-16E3||ICFC|
|Biotin anti-mouse IL-10||JES5-16E3||ELISA Detection, ELISPOT Detection, ICFC|
|FITC anti-mouse IL-10||JES5-16E3||ICFC|
|PE anti-mouse IL-10||JES5-16E3||ICFC|
|Purified anti-mouse IL-10||JES5-16E3||ELISA Capture, CyTOF®, IHC|
|Alexa Fluor® 647 anti-mouse IL-10||JES5-16E3||ICFC|
|PE/Cyanine7 anti-mouse IL-10||JES5-16E3||ICFC|
|Alexa Fluor® 488 anti-mouse IL-10||JES5-16E3||ICFC|
|Brilliant Violet 421™ anti-mouse IL-10||JES5-16E3||ICFC|
|Pacific Blue™ anti-mouse IL-10||JES5-16E3||ICFC|
|PerCP/Cyanine5.5 anti-mouse IL-10||JES5-16E3||ICFC|
|Purified anti-mouse IL-10 (Maxpar® Ready)||JES5-16E3||ELISA Capture, CyTOF®|
|Brilliant Violet 605™ anti-mouse IL-10||JES5-16E3||ICFC|
|PE/Dazzle™ 594 anti-mouse IL-10||JES5-16E3||ICFC|
|APC/Cyanine7 anti-mouse IL-10||JES5-16E3||ICFC|
|Ultra-LEAF™ Purified anti-mouse IL-10||JES5-16E3||ELISA Capture, CyTOF®, ELISPOT Capture, Neut, ICFC, IHC|
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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APC anti-mouse IL-10
Biotin anti-mouse IL-10
FITC anti-mouse IL-10
PE anti-mouse IL-10
Purified anti-mouse IL-10
Alexa Fluor® 647 anti-mouse IL-10
PE/Cyanine7 anti-mouse IL-10
Alexa Fluor® 488 anti-mouse IL-10
Brilliant Violet 421™ anti-mouse IL-10
Pacific Blue™ anti-mouse IL-10
PerCP/Cyanine5.5 anti-mouse IL-10
Purified anti-mouse IL-10 (Maxpar® Ready)
Brilliant Violet 605™ anti-mouse IL-10
PE/Dazzle™ 594 anti-mouse IL-10
APC/Cyanine7 anti-mouse IL-10
Ultra-LEAF™ Purified anti-mouse IL-10