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PerCP/Cyanine5.5 anti-human CD21 Antibody

Pricing & Availability
Clone
Bu32 (See other available formats)
Regulatory Status
RUO
Workshop
V CD21.4, VI CD21.5
Other Names
Complement C3d receptor (C3dR), complement receptor 2 (CR2), Epstein-Barr virus receptor
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
Bu32_PerCPCyanine55_CD21_Antibody_080918
Human peripheral blood lymphocytes were stained with CD19 APC and CD21 (clone Bu32) PerCP/Cyanine5.5 (left), or mouse IgG1, ? PerCP/Cyanine5.5 isotype control (right).
  • Bu32_PerCPCyanine55_CD21_Antibody_080918
    Human peripheral blood lymphocytes were stained with CD19 APC and CD21 (clone Bu32) PerCP/Cyanine5.5 (left), or mouse IgG1, ? PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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354907 25 tests 118€
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354908 100 tests 278€
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Description

CD21 is a 145 kD transmembrane protein also known as complement C3d receptor (C3dR), complement receptor 2 (CR2), and Epstein-Barr virus receptor. CD21 is expressed on B cells, follicular dendritic cells, subsets of normal thymocytes and T cells, and some epithelial cells. CD21 is the receptor used by Epstein-Barr virus to infect B cells and is also the complement receptor for C3d. CD21 has also been shown to interact with a number of proteins, including CD23, CD19, annexin VI, CD81, iC3b, complement receptor 1 (CR1, CD35), and interferon-alpha 1 (IFN-α1).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References
  1. Björck P, et al. 1993. Eur. J. Immunol. 23:1771.
  2. Frémeaux-Bacchi V, et al. 1996. Eur. J. Immunol. 26:1497.
  3. Ling NR, et al. 1995. Clin. Exp. Immunol. 101:369.
  4. Wang, C, et al. 2011. BMC Immunol. 12:53. (IHC)
Product Citations
  1. Wentink M, et al. 2015. Clin Immunol. Available online 30 August 2015. PubMed
  2. Zumaquero E, et al. 2019. Elife. 8. PubMed
  3. Johnson P, et al. 2015. Clin Cancer Res. 21:1321. PubMed
  4. Fu Y, et al. 2021. Cell Reports. 36(2):109344. PubMed
RRID
AB_2561543 (BioLegend Cat. No. 354907)
AB_2561544 (BioLegend Cat. No. 354908)

Antigen Details

Structure
Transmembrane protein, multiple SUSHI domains, approximate molecular weight 145 kD
Distribution

Expressed on B cells, follicular dendritic cells, subsets of normal thymocytes and T cells, some epithelial cells

Function
Epstein-Barr virus receptor on B cells used for infection
Interaction
CD23, CD19, complement component 3, annexin VI, CD81, iC3b, complement receptor 1, interferon alpha 1
Ligand/Receptor
C3d, EBV
Cell Type
B cells, Dendritic cells, Epithelial cells, T cells, Thymocytes
Biology Area
Cell Biology, Complement, Costimulatory Molecules, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Kishimoto T, Eds. 1997. Leukocyte Typing VI. Garland Publishing Inc.
2. Moore MD, et al. 1987. Proc. Natl. Acad. Sci. USA 84:9194.
3. Szakonyi G, et al. 2001. Science 292:1725.
4. Weis JJ, et al. 1984. Proc. Natl. Acad. Sci. USA 81:881.

Gene ID
1380 View all products for this Gene ID
UniProt
View information about CD21 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 08/09/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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