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PE/Cyanine7 anti-mouse CD3ε Recombinant Antibody

Pricing & Availability
Clone
QA17A05 (See other available formats)
Regulatory Status
RUO
Other Names
CD3ε, T3, CD3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA17A05_PECyanine7_CD3e_Antibody_082020
C57BL/6 mouse splenocytes were incubated with TruStain FcX™ (clone 93) and then stained with either CD3ε (clone QA17A05) PE/Cyanine7 (filled histogram) or mouse IgG1, κ PE/Cyanine7 isotype control (open histogram). Dead cells were excluded by Helix NP™ Blue.
  • QA17A05_PECyanine7_CD3e_Antibody_082020
    C57BL/6 mouse splenocytes were incubated with TruStain FcX™ (clone 93) and then stained with either CD3ε (clone QA17A05) PE/Cyanine7 (filled histogram) or mouse IgG1, κ PE/Cyanine7 isotype control (open histogram). Dead cells were excluded by Helix NP™ Blue.
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
155705 25 µg 76€
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155706 100 µg 169€
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Description

CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

QA17A05 blocks clone 145-2C11, QA17A05 activates T cell proliferation similar to the clone 145-2C11. This recombinant antibody clone is derived from clone 145-2C11.

Product Citations
  1. He S, et al. 2021. Front Pharmacol. 12:743837. PubMed
RRID
AB_2876517 (BioLegend Cat. No. 155705)
AB_2876517 (BioLegend Cat. No. 155706)

Antigen Details

Structure
Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
TCR signal transduction, T cell activation, antigen recognition
Ligand/Receptor
Peptide antigen/MHC-complex
Cell Type
NKT cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References
  1. Barclay AN, Brown MH. 1997. Biochem. Soc. Trans. 25:224-8.
  2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
  3. Weiss A, Littman DR. 1994. Cell. 76:263.
Gene ID
12501 View all products for this Gene ID
UniProt
View information about CD3epsilon on UniProt.org
Go To Top Version: 1    Revision Date: 08/20/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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