PE/Fire™ 810 anti-mouse NK-1.1 Antibody, NK-1.1, PK136

Pricing & Availability

Clone: PK136 (See other available formats)
Regulatory Status: RUO
Other Names: NKR-P1C, NKR-P1B, Ly-55, CD161, CD161b, CD161c
Isotype: Mouse IgG2a, κ
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Product Citations: publications

PK136_PE-FIRE-810_NK-11_Antibody_111722 — C57BL/6 mouse splenocytes were stained with anti-mouse CD49b FITC and anti-mouse NK1.1 (clone PK136) PE/Fire™ 810 (left), or stained with anti-mouse CD49b FITC only (right).

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108767

25 µg

116€

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Description

NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

NK-1⁺ cells from mouse spleen and bone marrow

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 810 under optimal conditions.

Concentration

0.2 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Fire™ 810 has a maximum excitation of 488/561 nm and a maximum emission of 810 nm.

Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect PE/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation^1,2, complement-dependent cytotoxicity³, in vivo depletion^4,5,9,10, mediation of in vitro redirected lysis⁶, blocking of NK cell function⁷, induction of proliferation⁸, immunohistochemical staining of frozen sections¹¹, immunofluorescence microscopy¹¹, and spatial biology (IBEX)^16,17. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 108712).

Application References

Carlyle JR, et al. 1999. J. Immunol. 162:5917. (IP)
Sentman CL, et al. 1989. Hybridoma 8:605. (IP)
Koo GC, et al. 1984. Hybridoma 3:301. (Cyt)
Sentman CL, et al. 1989. J. Immunol. 142:1847. (Deplete)
Koo GC, et al. 1986. J. Immunol. 137:3742. (Deplete)
Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
Kung SK, et al. 1999. J. Immunol. 162:5876. (Block)
Reichlin A, et al. 1998. Immunol. Cell Biol. 76:143.
Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (Deplete)
Kanwar JR, et al. 2001. J. Natl. Cancer Inst. 93:1541. (IHC, IF)
Kroemer A, et al. 2008. J. Immunol. 180:7818. PubMed
Kim JY, et al. 2009. Exp Mol Med. 30:288. PubMed
Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
Lee H, et al. 2014. Invest Ophthalmol Vis Sci. 55:2885. PubMed
Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

RRID

AB_2936526 (BioLegend Cat. No. 108767)

Antigen Details

Structure: NKR-P1 gene family
Distribution: NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)
Function: NK cell activation, IFN-γ production, cytotoxic granule release
Cell Type: NK cells, NKT cells
Biology Area: Immunology, Innate Immunity
Antigen References: 1. Lanier LL. 1997. Immunity 6:371.
2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613.
3. Koo GC, et al. 1986. J. Immunol. 137:3742.
4. Giorda R, et al. 1991. J. Immunol. 147:1701.
Gene ID: 17059 View all products for this Gene ID
UniProt: View information about NK-1.1 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All NK-1.1 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-mouse NK-1.1	PK136	FC
Biotin anti-mouse NK-1.1	PK136	FC
FITC anti-mouse NK-1.1	PK136	FC
PE anti-mouse NK-1.1	PK136	FC
Purified anti-mouse NK-1.1	PK136	FC,CyTOF®,IP,Depletion,Block,IHC-F
PE/Cyanine7 anti-mouse NK-1.1	PK136	FC
PE/Cyanine5 anti-mouse NK-1.1	PK136	FC
Alexa Fluor® 488 anti-mouse NK-1.1	PK136	FC
Alexa Fluor® 647 anti-mouse NK-1.1	PK136	FC
Pacific Blue™ anti-mouse NK-1.1	PK136	FC
Brilliant Violet 711™ anti-mouse NK-1.1	PK136	FC
APC/Cyanine7 anti-mouse NK-1.1	PK136	FC
PerCP anti-mouse NK-1.1	PK136	FC
PerCP/Cyanine5.5 anti-mouse NK-1.1	PK136	FC
Alexa Fluor® 700 anti-mouse NK-1.1	PK136	FC
Brilliant Violet 421™ anti-mouse NK-1.1	PK136	FC,SB
Brilliant Violet 570™ anti-mouse NK-1.1	PK136	FC
Brilliant Violet 650™ anti-mouse NK-1.1	PK136	FC
Brilliant Violet 510™ anti-mouse NK-1.1	PK136	FC
Brilliant Violet 605™ anti-mouse NK-1.1	PK136	FC
Purified anti-mouse NK-1.1 (Maxpar® Ready)	PK136	FC,CyTOF®
PE/Dazzle™ 594 anti-mouse NK-1.1	PK136	FC
Brilliant Violet 785™ anti-mouse NK-1.1	PK136	FC
APC/Fire™ 750 anti-mouse NK-1.1	PK136	FC
TotalSeq™-A0118 anti-mouse NK-1.1	PK136	PG
Ultra-LEAF™ Purified anti-mouse NK-1.1	PK136	FC,CyTOF®,IP,Depletion,Block,IHC-F
TotalSeq™-B0118 anti-mouse NK-1.1	PK136	PG
TotalSeq™-C0118 anti-mouse NK-1.1	PK136	PG
PE/Fire™ 810 anti-mouse NK-1.1	PK136	FC
PE/Fire™ 640 anti-mouse NK-1.1	PK136	FC
Spark UV™ 387 anti-mouse NK-1.1	PK136	FC
PE/Fire™ 700 anti-mouse NK-1.1	PK136	FC

Certificate of Analysis

Go To Top Version: 2 Revision Date: 05/03/2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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