- RMT3-23 (See other available formats)
- Regulatory Status
- Other Names
- T cell immunoglobulin and mucin domain containing 3 protein, hepatitis virus cellular receptor 2, CD366
- Rat IgG2a, κ
- Ave. Rating
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- Product Citations
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CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on Th1 lymphocytes and CD11b+ macrophages. Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 810 under optimal conditions.
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Fire™ 810 has a maximum excitation of 488/561 nm and a maximum emission of 810 nm.
Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect PE/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for relevant formats) include: in vitro1 and in vivo2 blocking of Tim-3, and immunohistochemical staining of frozen sections2. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 119731-119736).
- Application References
- Nakae S, et al. 2007. Blood 110(7):2565-8. (FC, Block)
- Oikawa T, et al. 2006. J. Immunol. 177(7):4281-7. (FC, Block, IHC)
AB_2922462 (BioLegend Cat. No. 119745)
- Transmembrane protein containing immunoglobulin domain and mucin-like domain; predicted molecular weight 31 kD; can exist as a soluble form lacking mucin and transmembrane domains
Expressed on activated Th1 lymphocytes and CD11b+ macrophages
- May play a role in the development of immune responses and the development of Th1-mediated responses
- Putative ligand on resting CD4+ lymphocytes
- Cell Type
- Macrophages, Th1
- Biology Area
- Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
1. Sabatos CA, et al. 2003. Nat. Immunol. 4:1102.
2. Sanchez-Fueyo A, et al. 2003. Nat. Immunol. 4:1102.
3. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454.
4. Mooney L, et al. 2002. Nature 415:536.
- Gene ID
- 171285 View all products for this Gene ID
- View information about CD366 on UniProt.org
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-mouse CD366 (Tim-3)
PE anti-mouse CD366 (Tim-3)
APC anti-mouse CD366 (Tim-3)
PE/Cyanine7 anti-mouse CD366 (Tim-3)
Biotin anti-mouse CD366 (Tim-3)
PerCP/Cyanine5.5 anti-mouse CD366 (Tim-3)
GoInVivo™ Purified anti-mouse CD366 (Tim-3)
Brilliant Violet 605™ anti-mouse CD366 (Tim-3)
Brilliant Violet 421™ anti-mouse CD366 (Tim-3)
Brilliant Violet 711™ anti-mouse CD366 (Tim-3)
Brilliant Violet 785™ anti-mouse CD366 (Tim-3)
TotalSeq™-A0003 anti-mouse CD366 (Tim-3)
Ultra-LEAF™ Purified anti-mouse CD366 (Tim-3)
APC/Fire™ 750 anti-mouse CD366 (Tim-3)
TotalSeq™-C0003 anti-mouse CD366 (Tim-3)
TotalSeq™-B0003 anti-mouse CD366 (Tim-3)
Alexa Fluor® 647 anti-mouse CD366 (Tim-3)
PE/Fire™ 810 anti-mouse CD366 (Tim-3)
PE/Dazzle™ 594 anti-mouse CD366 (Tim-3)
PE/Fire™ 640 anti-mouse CD366 (Tim-3)