PE/Cyanine7 anti-mouse Ly-6G Antibody

Pricing & Availability
Clone
1A8 (See other available formats)
Other Names
Lymphocyte antigen 6 complex, locus G
Isotype
Rat IgG2a, κ
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Product Citations
publications
1A8_PECy7_092409
C57BL/6 bone marrow cells stained with 1A8 PE/Cyanine7
  • 1A8_PECy7_092409
    C57BL/6 bone marrow cells stained with 1A8 PE/Cyanine7
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Avail. Save
127617 25 µg 72€
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127618 100 µg 220€
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Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, and depletion4, 12-14. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 127620). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 127632) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
Product Citations
  1. Liao Y, et al. 2017. Virol J. 10.1186/s12985-017-0801-x. PubMed
  2. Zukauskas A, et al. 2018. mSphere. 3:e00303. PubMed
  3. Thurlow LR, et al. 2018. Cell Host Microbe. 24:261. PubMed
  4. White JP et al. 2018. Cell. 175(5):1198-1212 . PubMed
  5. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  6. Chhatbar C et al. 2018. Cell reports. 25(1):118-129 . PubMed
  7. Braza MS et al. 2018. Immunity. 49(5):819-828 . PubMed
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  9. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  10. Geng S, et al. 2019. Sci Adv. 5:eaav2309. PubMed
  11. Buxadé M, et al. 2018. J Exp Med. 215:2901. PubMed
  12. Laura C Burzynski et al. 2019. Immunity. 50(4):1033-1042 . PubMed
  13. Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
  14. Kim TJ, et al. 2019. Nat Commun. 10:3258. PubMed
  15. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
  16. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  17. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  18. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  19. Dmitrieva‐Posocco O et al. 2019. Immunity. 50(1):166-180 . PubMed
  20. Mogilenko DA et al. 2019. Cell. 177(5):1201-1216 . PubMed
  21. Zhou J, et al. 2019. Cell Stem Cell. 24:285. PubMed
  22. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  23. Pan W et al. 2017. Immunity. 47(2):284-297 . PubMed
  24. Riffelmacher T et al. 2017. Immunity. 47(3):466-480 . PubMed
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  26. Sun KT, et al. 2018. Front Physiol. 1.06875. PubMed
  27. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
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  29. Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
  30. Cheng Y, et al. 2019. Clin Cancer Res. 25:2621. PubMed
  31. Katsura A, et al. 2017. Mol Oncol. 11:1241. PubMed
  32. Ngamsri KC, et al. 2020. Front Immunol. 11:407. PubMed
  33. Shook BA, et al. 2020. Cell Stem Cell. 26(6):880-895. PubMed
  34. Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed
  35. Li Y, et al. 2020. Cell Reports. 27(10):2809-2816.e3.. PubMed
  36. Nguyen GT, et al. 2020. eLife. 9:e56656.. PubMed
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  39. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  40. Lin R, et al. 2020. Sci Rep. 10:14397. PubMed
  41. Fischer KJ, et al. 2019. J Immunol. 202:2027. PubMed
  42. Chan C, et al. 2012. Hypertension. 60:1207. PubMed
  43. Park Y, et al. 2013. Cancer Res . 73:5669. PubMed
  44. Johansson J, et al. 2013. J Neurosci. 33:16016. PubMed
  45. Bryant J, et al. 2014. J Immunol. 192:6092. PubMed
  46. Coquery C, et al. 2014. PLoS One. 9:102284. PubMed
  47. Furuya Y, et al. 2014. J Virol. 88:9166. PubMed
  48. Zasłona Z, et al. 2014. J Immunol. 193:4245. PubMed
  49. Bieren J, et al. 2015. J Immunol. 194:1154. PubMed
  50. Mylonas K, et al. 2015. Immunobiology. 220: 924-933. PubMed
  51. Galvani S, et al. 2015. Sci Signal. 8: ra79. PubMed
  52. AM C, et al. 2015. Proc Natl Acad Sci U S A. 112 7557 . PubMed
  53. Moore J, et al. 2015. Am J Physiol Heart Circ Physiol. 309: 906-917. PubMed
  54. Furuya Y, et al. 2015. PLoS Pathog. 11: 1005180. PubMed
  55. Douglas T, et al. 2015. J Immunol. 195: 2365-2373. PubMed
  56. Gabbita S, et al. 2015. PLoS One. 10: e0137305. PubMed
  57. Lee J, et al. 2015. Nat Commun. 6: 8499. PubMed
  58. Dudek M, et al. 2015. Mucosal Immunol. . PubMed
  59. Almishri W, et al. 2015. J Hepatol. 63: 943-951. PubMed
  60. Sanfilippo A, et al. 2015. Infect Immun . 83: 2976-2983. PubMed
  61. Yin S, et al. 2016. Nat Commun. 7: 11311. PubMed
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  63. Chen F, et al. 2016. J Immunol. 196: 4390 - 4399. PubMed
  64. Upadhya R, et al. 2016. MBio. 7: 00547-16. PubMed
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RRID
AB_1877262 (BioLegend Cat. No. 127617)
AB_1877261 (BioLegend Cat. No. 127618)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 01/06/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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