Loading...

PE/Cyanine7 anti-mouse I-A/I-E Antibody

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
M5114_PECy7_092409
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) PE/Cyanine7 (orange line) or rat IgG2b, κ PE/Cyanine7 isotype control (purple line).
  • M5114_PECy7_092409
    C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) PE/Cyanine7 (orange line) or rat IgG2b, κ PE/Cyanine7 isotype control (purple line).
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
107629 25 µg 76€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
107630 100 µg 234€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  17. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  18. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Shi M, et al. 2021. Theranostics. 11:2349. PubMed
  2. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  3. Zhao J, et al. 2021. Emerg Microbes Infect. 10:913. PubMed
  4. Li Y et al. 2017. EBioMedicine. 18:188-198 . PubMed
  5. Rezende R, et al. 2015. Nat Commun. 6: 8726. PubMed
  6. Pandey S, et al. 2020. Cell Syst. 11:495. PubMed
  7. Rosa FF, et al. 2020. Bio Protoc. 10:e3619. PubMed
  8. Kim TJ, et al. 2019. Nat Commun. 10:3258. PubMed
  9. Pingili AK, et al. 2021. Cell Reports. 35(12):109285. PubMed
  10. Nguyen SL, et al. 2021. Sci Rep. 11:4217. PubMed
  11. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  12. Grohmann M et al. 2018. Cell. 175(5):1289-1306 . PubMed
  13. Zhang J, et al. 2021. J Leukoc Biol. 110:485. PubMed
  14. Rolfes S, et al. 2020. Neurobiol Dis. 144:105024. PubMed
  15. Wang Z, et al. 2021. J Virol. 95:e0141421. PubMed
  16. Sheng K, et al. 2014. PLoS One. 9:95208. PubMed
  17. Wei SC, et al. 2020. Cancer Discov. . PubMed
  18. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  19. Li J, et al. 2020. Cancer Immunol Res. 0.529166667. PubMed
  20. Domeier P, et al. 2016. J Exp Med. 213: 715 - 732. PubMed
  21. Soni C, et al. 2014. J Immunol. 193:4400. PubMed
  22. Lagares D, et al. 2017. Sci Transl Med. 9:eaal3765. PubMed
  23. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  24. Li J, et al. 2018. Immunity. 49:178. PubMed
  25. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  26. Yang J, et al. 2020. Nature. 586:572. PubMed
  27. Ahadome S, et al. 2016. JCI Insight. 1:e87012. PubMed
  28. Jager SE, et al. 2020. Glia. 68(7):1375-1395. PubMed
  29. Horiguchi H, et al. 2019. Genes Dev. 33:1641. PubMed
  30. Tan X, et al. 2021. Sci Adv. 7: . PubMed
  31. Zeng W, et al. 2021. STAR Protocols. 2(1):100361. PubMed
  32. Toomey CB, et al. 2018. Invest Ophthalmol Vis Sci. 59:662. PubMed
  33. Seki T, et al. 2015. J Biochem. 158: 485 - 495. PubMed
  34. Sydney Lavoie et al. 2019. eLife. 8 pii: e39982. PubMed
  35. Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
  36. Hu Y, et al. 2021. Cell Death Dis. 12:743. PubMed
  37. Mossadegh–Keller N, et al. 2019. Bio Protoc. 9:5. PubMed
  38. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
  39. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  40. He C, et al. 2022. Nat Commun. 13:5459. PubMed
  41. Williams JB, et al. 2020. Nat Commun. 0.876388889. PubMed
  42. Rosenbaum SR, et al. 2020. Cell Rep. 30:510. PubMed
  43. Kriegsman BA, et al. 2019. J Immunol. 203:1999. PubMed
  44. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  45. Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
  46. Wei Y, et al. 2021. Endocrinology. 162(8):. PubMed
  47. Natale CA, et al. 2018. Elife. 7. PubMed
  48. Wong E, et al. 2015. J Immunol. 194:4130. PubMed
  49. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  50. Chen B, et al. 2021. Cell. 184:6262. PubMed
  51. Guan D, et al. 2021. Cell Death Dis. 12:431. PubMed
  52. Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed
  53. Maller O, et al. 2020. Nat Mater. 20:548. PubMed
  54. Henkle TR, et al. 2021. Cancer Res. 81:4560. PubMed
  55. Rappe JCF, et al. 2021. J Exp Med. 218:. PubMed
  56. Dourcy M, et al. 2020. Mucosal Immunol. 13:799. PubMed
  57. Zhang M, et al. 2022. iScience. 25:104490. PubMed
  58. Li CY, et al. 2022. Int J Mol Sci. 23:. PubMed
  59. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
RRID
AB_2290801 (BioLegend Cat. No. 107629)
AB_2069376 (BioLegend Cat. No. 107630)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All I-A/I-E Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse I-A/I-E M5/114.15.2 FC
FITC anti-mouse I-A/I-E M5/114.15.2 FC
PE anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
PE/Cyanine5 anti-mouse I-A/I-E M5/114.15.2 FC
APC anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 488 anti-mouse I-A/I-E M5/114.15.2 FC,3D IHC
Alexa Fluor® 647 anti-mouse I-A/I-E M5/114.15.2 FC
Pacific Blue™ anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 700 anti-mouse I-A/I-E M5/114.15.2 FC,SB
PerCP/Cyanine5.5 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP anti-mouse I-A/I-E M5/114.15.2 FC
APC/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 421™ anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,SB
Brilliant Violet 510™ anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E (Maxpar® Ready) M5/114.15.2 FC,CyTOF®
Brilliant Violet 605™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 650™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 711™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 785™ anti-mouse I-A/I-E M5/114.15.2 FC
PE/Dazzle™ 594 anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 594 anti-mouse I-A/I-E M5/114.15.2 IHC-F,3D IHC
APC/Fire™ 750 anti-mouse I-A/I-E M5/114.15.2 FC
TotalSeq™-A0117 anti-mouse I-A/I-E M5/114.15.2 PG
Ultra-LEAF™ Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
TotalSeq™-B0117 anti-mouse I-A/I-E M5/114.15.2 PG
TotalSeq™-C0117 anti-mouse I-A/I-E M5/114.15.2 PG
Spark Blue™ 550 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 640 anti-mouse I-A/I-E M5/114.15.2 FC
Spark YG™ 581 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark UV™ 387 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Violet™ 538 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP/Fire™ 806 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Red™ 718 anti-mouse I-A/I-E M5/114.15.2 FC
APC/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Go To Top Version: 4    Revision Date: 10/30/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account