PE/Cyanine7 anti-human LGR5 (GPR49) Antibody

Pricing & Availability
Clone
SA222C5 (See other available formats)
Regulatory Status
RUO
Other Names
G-Protein coupled receptor 49 (GPR49), G-Protein coupled receptor 67 (GPR67), FEX, HG38
Isotype
Mouse IgG2a, κ
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Product Citations
publications
SA222C5_PE_Cyanine7_LGR5_Antibody_030222
Human LGR5 transfected Ba/F3 cells were stained with anti-human LGR5 (GPR49) (clone SA222C5) PE/Cyanine7 (filled histogram) or mouse IgG2a, κ PE/Cyanine7 isotype control (open histogram).
  • SA222C5_PE_Cyanine7_LGR5_Antibody_030222
    Human LGR5 transfected Ba/F3 cells were stained with anti-human LGR5 (GPR49) (clone SA222C5) PE/Cyanine7 (filled histogram) or mouse IgG2a, κ PE/Cyanine7 isotype control (open histogram).
Compare all formats See PE/Cyanine7 spectral data
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373807 25 tests 155€
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373808 100 tests 322€
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Description

LGR5, also known as GPR49, is a 100 KD protein. It is a member of GPCR class A orphan receptor proteins which binds R-spondin and does not activate through heterotrimeric G protein like other GPCR. It triggers the Wnt signaling pathway downstream. LGR5 is expressed across a diverse range of tissue such as in the muscle, placenta, spinal cord and brain and particularly as a biomarker of adult stem cells in certain tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human LGR5 transfectant cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2922590 (BioLegend Cat. No. 373807)
AB_2922590 (BioLegend Cat. No. 373808)

Antigen Details

Structure
G-protein coupled receptor (GPCR) superfamily
Distribution

Muscle, placenta, spinal cord, brain and particularly as a biomarker of adult stem cells in certain tissues

Function
Triggers Wnt signaling pathway
Interaction
Wnt pathway
Ligand/Receptor
R-spondins (RSpo 1,2,3, and 4)
Biology Area
Immunology
Antigen References
  1. Hsu Sy, et al. 1998. Mol. Endocrinol. 12:1830.
  2. Barker N, et al. 2007. Nature. 449:1003.
  3. Morita H, et al. 2004. Mol. Cell. Biol. 24(22):9736.
  4. Arioka Y, et al. 2017. Stem Cell Res. 20:1.
  5. Wang B, et al. 2016. Plos. One. 11:e0168904.
  6. Garcia MI, et al. 2009. Dev. Biol. 331:58.
Gene ID
8549 View all products for this Gene ID
UniProt
View information about LGR5 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03/01/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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