- DATK32 (See other available formats)
- Regulatory Status
- Other Names
- α4β7 Integrin, CD49d/β7, LPAM-1, ITGA4, ITGB7
- Rat IgG2a, κ
- Ave. Rating
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- Product Citations
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DATK32 antibody is specific for a combinatorial determinate of integrin α4β7complex. Integrin α4β7 is composed of a 150 kD (α4 or CD49d) and a 130 kD (β7) heterodimer, also known as CD49d/β7 or LPAM-1. Belonging to the Ig superfamily, it is found on the majority of peripheral lymphocytes and subsets of thymocytes and bone marrow cells (including mast cell progenitors). Integrin α4β7 binds its ligands, VCAM-1 (CD106), MAdCAM-1 and fibronectin, and plays an important role in lymphocytes adhesion and the direction of migration of blood lymphocytes to the intestine and associated lymphoid tissues.Product Details
- Antibody Type
- Host Species
- TK1 cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell adhesion1-4, in vitro induction of TK1 cell aggregation1, immunoprecipitation4, and immunohistochemical staining of frozen sections5. It has been reported that the DATK32 mAb is able to block a4ß7 mediated lymphocyte adhesion to VCAM-1, MAdCAM-1, and fibronectin in vitro and in vivo.
- Application References
- Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block)
- Berlin C, et al. 1993. Cell 74:185. (Block)
- Berlin-Rufenach C, et al. 1999. J. Exp. Med. 189:1467. (Block)
- Rivera-Nieves J, et al. 2005. J. Immunol. 174:2343. (Block, IP)
- Bogetto L, et al. 2000. Blood 95:2397. (IHC)
- Product Citations
AB_493268 (BioLegend Cat. No. 120605)
AB_493267 (BioLegend Cat. No. 120606)
- Integrin family, Ig superfamily, 150 kD/130 kD heterodimer
Majority of peripheral lymphocytes, subsets of thymocytes and bone marrow cells
- Lymphocyte adhesion
- VCAM-1 (CD106), MAdCAM-1, fibronectin
- Cell Type
- Lymphocytes, Thymocytes
- Biology Area
- Cell Biology, Immunology, Neuroscience, Synaptic Biology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Andrew DP, et al. 1994. J. Immunol. 153:3847.
2. Berlin C, et al. 1994. Cell 74:185.
3. Gurish MF, et al. 2001 J. Exp. Med. 194:1243.
4. Hamann A, et al. 1994. J. Immunol. 152:3282.
- Gene ID
- 311144 View all products for this Gene ID 25713 View all products for this Gene ID
- View information about LPAM-1 on UniProt.org
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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