PE anti-mouse IL-3 Antibody

Pricing & Availability
MP2-8F8 (See other available formats)
Regulatory Status
Other Names
Interleukin-3, Burst promoting activity, Eosinophil colony stimulating factor (Eo-CSF), Hematopoietic cell growth factor (HCGF), Mast (MGF/MCGF), Multi-colony stimulating (Multi-CSF), P cell stimulating activity (PCSA), Thy1 inducing factor
Rat IgG1, κ
Ave. Rating
Submit a Review
Product Citations
PMA+Ionomycin stimulated Th2-polarized C57BL/6 splenocytes surface stained with CD4 (GK1.5) APC and intracellularly stained with MP2-8F8 PE
  • MP2-8F8_PE_110209
    PMA+Ionomycin stimulated Th2-polarized C57BL/6 splenocytes surface stained with CD4 (GK1.5) APC and intracellularly stained with MP2-8F8 PE
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
503903 25 µg 88€
Check Availability

Need larger quantities of this item?
Request Bulk Quote
503904 100 µg 229€
Check Availability

Need larger quantities of this item?
Request Bulk Quote

IL-3 is a highly species-specific pleiotropic factor produced primarily by activated T cells though also by mast cells keratinocytes, and astrocytes, which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. The MP2-8F8 antibody reacts with mouse interleukin-3 (IL-3). The MP2-8F8 antibody can neutralize the bioactivity of natural or recombinant IL-3.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
COS-expressed, recombinant mouse IL-3
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA or ELISPOT Capture1-5,8: The purified MP2-8F8 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP2-43D11 antibody (Cat. No. 504002) as the detecting antibody. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.

Application References
  1. Abrams, J., et al. 1992. Immunol. Rev. 127:5.
  2. Abrams, J., et al. 1988. J. Immunol. 140:131.
  3. Cockayne, D., et al. 1991. Growth Factors 5:171.
  4. Sander, B., et al. 1993. J. Immunol. Meth. 166:201.
  5. Abrams, J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
  6. Finkelman, F., et al. 1993. J. Immunol. 151:1235.
  7. Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
  8. Karulin, A., et al. 2000. J. Immunol. 164:1862.
Product Citations
  1. Price J, et al. 2015. Diabetes. 64: 3521 - 3531. PubMed
AB_315309 (BioLegend Cat. No. 503903)
AB_315310 (BioLegend Cat. No. 503904)

Antigen Details

Cytokine; 28 kD (Mammalian)
Proliferation/differentiation of almost all types of hematopoietic progenitors into granulocytes, macrophages, erythroid cells, megakaryocytes, mast cell colonies; induces expression of 20-α-steroid dehydrogenase, histidine, ornithine decarboxylase
Cell Sources
Activated T cells, mast cells, eosinophils, keratinocytes, NK cells, endothelial cells
Cell Targets
Erythroid cells, megakaryocytes, neutrophils, eosinophils, basophils, mast cells, monocytic lineages
Heterodimer IL-3Rα (CD123) and two alternate β-subunits, AIC2A binds IL-3 and is specific to IL-3Rα, AIC2B (CDw131) shared in common with IL-5R, GM-CSFR
Biology Area
Molecular Family
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Frendl, G., 1992. Int. J. Immunopharmacol. 14:421.
3. Ihle, J., 1992. Chem. Immunology 51:65.
4. Valen T, P., et al. 1990. Blut 61:338.

Upregulated by T cell stimulation; downregulated by glucocorticoids
Gene ID
16187 View all products for this Gene ID
View information about IL-3 on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All IL-3 Reagents Request Custom Conjugation
Description Clone Applications
PE anti-mouse IL-3 MP2-8F8 ICFC
Purified anti-mouse IL-3 MP2-8F8 ELISA Capture,IHC,IP
APC anti-mouse IL-3 MP2-8F8 ICFC
Ultra-LEAF™ Purified anti-mouse IL-3 MP2-8F8 ELISA Capture,ELISPOT Capture,Neut
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


8999 BioLegend Way, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • PE anti-mouse IL-3

  • Purified anti-mouse IL-3

  • APC anti-mouse IL-3

  • Ultra-LEAF™ Purified anti-mouse IL-3


Login / Register
Remember me
Forgot your password? Reset password?
Create an Account