PE anti-mouse Ig light chain kappa

Pricing & Availability

Clone: RMK-45 (See other available formats)
Regulatory Status: RUO
Other Names: Immunoglobulin light chain kappa
Isotype: Rat IgG
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Product Citations: publications

RMK-45_PE_IgK_Antibody_FC_071614 — C57BL/6 splenocytes were stained with anti-mouse Ig light chain κ (clone RMK-45) PE (filled histogram) or rat IgG1, κ PE isotype control (open histogram).

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409505

50 µg

88€

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409506

200 µg

259€

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Description

The RMK-45 monoclonal antibody reacts with immunoglobulin light chain kappa in all tested mouse haplotype (Igh-a and b). It does not react with the lambda chain.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The RMK-12 monoclonal antibody may be used as a primary antibody for ELISA or as secondary for detection of mouse Ig kappa.
RRID: AB_2563580 (BioLegend Cat. No. 409505)
AB_2563581 (BioLegend Cat. No. 409506)

Antigen Details

Structure: Immunoglobulin family
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Protein Synthesis
Antigen References: 1. Ludwig TE, et al. 2006. Nat. Methods. 3:637.
2. Nguyen DH, et al. 2005. J. Immunol. 175:228.
3. Bardor M, et al. 2005. J. Biol. Chem. 280:4228.
4. Diaz SL, et al. 2009. PLoS One. 4:e4241.
Gene ID: 243469 View all products for this Gene ID
UniProt: View information about Ig light chain kappa on UniProt.org

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All Ig light chain κ Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse Ig light chain κ	RMK-45	FC,ELISA
APC/Cyanine7 anti-mouse Ig light chain κ	RMK-45	FC
PE anti-mouse Ig light chain κ	RMK-45	FC
Alexa Fluor® 700 anti-mouse Ig light chain κ	RMK-45	FC
FITC anti-mouse Ig light chain κ	RMK-45	FC

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Go To Top Version: 1 Revision Date: 07/28/2014

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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