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PE anti-mouse H-2Kb bound to SIINFEKL Antibody

Pricing & Availability
Clone
25-D1.16 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class I molecule Kb bound to the peptide SIINFEKL (Kb-SIINFEKL) H-2Kb, OVA agonist peptide (OVAp), OVA-Kb, OVA257-264 (SIINFEKL)-H-2kb, OVA257-264-H-2kb
Isotype
Mouse IgG1, κ
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Product Citations
publications
25-D1.16_PE_050411
C57BL/6 mouse splenocytes were pulsed with (filled histogram) or without (cyan open histogram) SIINFEKL for 2 hours and then stained with 25-D1.16 PE or mouse IgG1, κ PE isotype control (broken line open histogram).
  • 25-D1.16_PE_050411
    C57BL/6 mouse splenocytes were pulsed with (filled histogram) or without (cyan open histogram) SIINFEKL for 2 hours and then stained with 25-D1.16 PE or mouse IgG1, κ PE isotype control (broken line open histogram).
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141603 25 µg 88€
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141604 100 µg 288€
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Description

This antibody has been proven to be very useful in tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SIINFEKL pulsed RMA-S cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The 25-D1.16 monoclonal antibody specifically reacts with ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb or H-2Kb bound with an irrelevant peptide.  Additional reported applications (for relevant formats) include: immunofluorescence microscopy2,3, immunohistochemical staining of frozen tissue sections3 , and inhibition of T cell response to H-2Kb-SIINFEKL in vitro.

Application References
  1. Mareeva T, et al. 2010. J. Immunol. Methods 353:78. 
  2. Dolan BP, et al. 2010. J. Immunol. 184:1419. (IF)
  3. Porgador A, et al. 1997. Immunity 6:715. (IF, IHC)
  4. Herve J, et al. 2013. J. Immunol. 190:3163. PubMed.
Product Citations
  1. Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed
  2. Abusarah J, et al. 2021. Cell Rep Med. 2:100455. PubMed
  3. Kaczanowska S, et al. 2021. Cell. 184(8):2033-2052.e21. PubMed
  4. Sethumadhavan S, et al. 2017. PLoS One. . 10.1371/journal.pone.0187314. PubMed
  5. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 5:64. PubMed
  6. Hu CF, et al. 2021. Vaccines (Basel). 9: . PubMed
  7. Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed
  8. Walter F, et al. 2020. PLoS One. 15:e0239369. PubMed
  9. Klatt MG, et al. 2020. J Proteomics. 228:103921. PubMed
  10. Guo J, et al. 2019. PLoS One. 14:e0219829. PubMed
  11. Jiang Y, et al. 2020. Adv Mater. 32:e2001808. PubMed
  12. Dai R, et al. 2021. Front Immunol. 12:727046. PubMed
  13. Hervé J, et al. 2013. J Immunol. 190:3163. PubMed
  14. Mohamed E, et al. 2020. Immunity. 52(4):668-682.e7.. PubMed
  15. Gong N, et al. 2020. Nat Nanotechnol. 1.35625. PubMed
  16. Xu DD, et al. 2020. Vaccines (Basel). 8:00. PubMed
  17. Xia Y, et al. 2018. Cell. 175:1059. PubMed
  18. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  19. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  20. Kataru RP, et al. 2019. Cancer Immunol Res. 7:1345. PubMed
  21. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 0.252777778. PubMed
  22. Song S, et al. 2021. Front Immunol. 12:705140. PubMed
  23. Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed
  24. Zhou Q, et al. 2016. Sci Rep. 6:39071. PubMed
RRID
AB_10897938 (BioLegend Cat. No. 141603)
AB_10895905 (BioLegend Cat. No. 141604)

Antigen Details

Structure
OVA peptide257-264 and H-2Kb complex
Distribution

SIINFEKL bond H-2Kb bearing cells

Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Mareeva T, et al. 2010. J. Immunol. Methods 353:78.
2. Mareeva T, et al. 2008. J. Biol. Chem. 283:29053.
3. Deng Y, et al. 1998. J. Immunol. 161:1677.

Gene ID
14972 View all products for this Gene ID
UniProt
View information about H-2Kb bound to SIINFEKL on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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