PE anti-mouse CD31 Antibody

Pricing & Availability
Clone
390 (See other available formats)
Regulatory Status
RUO
Other Names
PECAM-1, EndoCAM
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
390
C57BL/6 splenocytes stained with 390 PE
  • 390
    C57BL/6 splenocytes stained with 390 PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
102407 50 µg 72,00€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
102408 200 µg 200,00€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1) and EndoCAM. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (αv β3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation and angiogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/HeJ mouse hematopoietic progenitor cell line 3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102412).

Application References
  1. Baldwin HS, et al. 1994. Development 120:2539. (IP, Block)
  2. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
  3. Rosenblum WI, et al. 1996. Stroke 27:709. (Block)
  4. Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block)
  5. Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC)
  6. Wiewrodt R, et al. 2002. Blood 99:912. (IHC)
  7. McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed
  8. Chacko AM, et al. 2012. PLoS One 7:e34958.
  9. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
Product Citations
  1. Fokas E, et al. 2012. Cancer Res. 72:239. PubMed
  2. Reymond N, et al. 2012. J Cell Biol. 199:653. PubMed
  3. Baker M, et al. 2013. PLoS One. 8:62516. PubMed
  4. Aschermann S, et al. 2013. Proc Natl Acad Sci U S A. 110:19042. PubMed
  5. Im J, et al. 2012. Int J Cancer. 132:315. PubMed
  6. Moding E, et al. 2015. Sci Transl Med. 7:278. PubMed
  7. Groppa E, et al. 2015. EMBO Mol Med. 2015; 7: 1366 - 1384. PubMed
  8. Sun L, et al. 2015. Sci Rep. 5: 14871. PubMed
  9. Nakagawa A, et al. 2016. Sci Rep. 6: 25009. PubMed
  10. Caporarello N, et al. 2020. Aging Cell. 19:e13196. PubMed
  11. Wong PP, et al. 2020. Cell. 181(6):1346-1363.e21. PubMed
  12. Sun D, et al. 2021. Cell Stem Cell. . PubMed
  13. Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed
  14. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
  15. Zhao T et al. 2018. Cell stem cell. 23(1):31-45 . PubMed
  16. Wang QA et al. 2018. Cell metabolism. 28(2):282-288 . PubMed
  17. Padhye A, et al. 2019. Sci Rep. 9:4819. PubMed
  18. Lucotti S, et al. 2019. J Clin Invest. 130:1845. PubMed
  19. Dubey LK, et al. 2019. Cell Rep. 27:2442. PubMed
  20. Suan D et al. 2017. Immunity. 47(6):1142-1153 . PubMed
  21. Toyama K, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1392. PubMed
  22. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  23. Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed
  24. Hepler C et al. 2017. eLife. 6 pii: e27669. PubMed
  25. Santini MP, et al. 2020. Cell Rep. 30:555. PubMed
  26. Hou M, et al. 2020. Stem Cell Res Ther. 11:55. PubMed
  27. Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
  28. Watari K, et al. 2020. Commun Biol. 3:107. PubMed
  29. Demircioglu F, et al. 2020. Nat Commun. 11:1290. PubMed
  30. Chen H, et al. 2020. Sci Adv. 6:eaba4311. PubMed
  31. Michael BD, et al. 2020. Cell Reports. 32(11):108150. PubMed
  32. Stine RR, et al. 2020. Cell Stem Cell. 25(6):830-845. PubMed
  33. Wang L, et al. 2008. Cancer Res. 68:5639. PubMed
  34. Qayum N, et al. 2009. Cancer Res. 69:6347. PubMed
  35. Chong J, et al. 2010. J Biol Chem. 285:36179. PubMed
RRID
AB_312902 (BioLegend Cat. No. 102407)
AB_312903 (BioLegend Cat. No. 102408)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.

Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 07/19/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Alexa Fluor® 488 anti-mouse CD31

  • Alexa Fluor® 647 anti-mouse CD31

  • Brilliant Violet 421™ anti-mouse CD31

  • APC/Fire™ 750 anti-mouse CD31

  • Alexa Fluor® 700 anti-mouse CD31

  • PerCP/Cyanine5.5 anti-mouse CD31

  • Pacific Blue™ anti-mouse CD31

  • Alexa Fluor® 594 anti-mouse CD31

  • TotalSeq™-C0904 anti-mouse CD31

  • TotalSeq™-B0904 anti-mouse CD31 Antibody

  • Brilliant Violet 711™ anti-mouse CD31

  • PE/Cyanine7 anti-mouse CD31

  • Brilliant Violet 605™ anti-mouse CD31

  • TotalSeq™-A0904 anti-mouse CD31

  • APC/Cyanine7 anti-mouse CD31

  • Spark YG™ 570 anti-mouse CD31

  • PE/Dazzle™ 594 anti-mouse CD31

  • Brilliant Violet 785™ anti-mouse CD31

  • Ultra-LEAF™ Purified anti-mouse CD31

  • APC anti-mouse CD31

  • Biotin anti-mouse CD31

  • FITC anti-mouse CD31

  • Purified anti-mouse CD31

  • Purified anti-mouse CD31 (Maxpar® Ready)

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account