Loading...

PE anti-mouse CD206 (MMR) Antibody

Pricing & Availability
Clone
C068C2 (See other available formats)
Regulatory Status
RUO
Other Names
MMR (macrophage mannose receptor), MR (mannose receptor), MRC1
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
C068C2_PE_1_082911
Thioglycollate-elicited BALB/c peritoneal macrophages were surface stained with CD107b (Mac-3) APC, and then intracellularly stained with CD206 (clone C068C2) PE (top) or rat IgG2a, κ PE isotype control (bottom).
  • C068C2_PE_1_082911
    Thioglycollate-elicited BALB/c peritoneal macrophages were surface stained with CD107b (Mac-3) APC, and then intracellularly stained with CD206 (clone C068C2) PE (top) or rat IgG2a, κ PE isotype control (bottom).
  • C068C2_PE_2_082911
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
141705 25 µg 104€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
141706 100 µg 240€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD206, also known as mannose receptor (MR), is a 175 kD type I membrane protein. It is a pattern recognition receptor (PRR) belonging to the C-type lectin superfamily. MR is expressed on macrophages, dendritic cells, Langerhans cells, and hepatic or lymphatic endothelial cells. MR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine through its C-type lectin-like carbohydrate recognition domains, sulfated carbohydrate antigens through its cysteine-rich domain, and collagens through its fibronectin type II domain. MR mediates endocytosis and phagocytosis as well as activation of macrophages and antigen presentation. It plays an important role in host defense and provides a link between innate and adaptive immunity. Recently, MR on lymphatic endothelial cells was found to be involved in leukocyte trafficking and a contributor to the metastatic behavior of cancer cells. It suggests that MR may be a potential target in controlling inflammation and cancer metastasis by targeting the lymphatic vasculature.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD206 (MMR)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone C068C2 recognizes a region similar to clone MR5D3, based on the ability of the clones to block each other. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.

Application References
  1. Keller J, et al. 2012. Biochem Biophys Res Commun. 417:217. PubMed
  2. Ito H, et al. 2012. J Am Soc Nephrol. 23:1797. PubMed
  3. Yang X, et al. 2015. PNAS. 112:2900. PubMed
  4. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
  2. Feng Y, et al. 2018. J Am Soc Nephrol. 29:182. PubMed
  3. Seo JB, et al. 2019. Nat Metab. 1:86. PubMed
  4. Ying W, et al. 2019. Cell Metab. 29:457. PubMed
  5. Sun H, et al. 2019. Mol Hum Reprod. 25:05:00. PubMed
  6. Xiong Y, et al. 2020. J Nanobiotechnology. 18:66. PubMed
  7. Cui J, et al. 2021. J Cell Mol Med. 25:5586. PubMed
  8. Zhang B, et al. 2022. Front Med (Lausanne). 8:796743. PubMed
  9. Wang HH, et al. 2022. J Biomed Sci. 29:36. PubMed
  10. Zhang Z, et al. 2022. Front Pharmacol. 13:906625. PubMed
  11. Hu S, et al. 2018. Front Immunol. 9:01. PubMed
  12. Ying W et al. 2017. Cell. 171(2):372-384 . PubMed
  13. Tripathi H, et al. 2020. J Mol Cell Cardiol. 149:95. PubMed
  14. Nomura M, et al. 2016. Nat Immunol. 17:216-217. PubMed
  15. Leach SM, et al. 2020. Cell Rep. 33:108337. PubMed
  16. Zheng W, et al. 2021. Wellcome Open Research. 6:52. PubMed
  17. Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed
  18. Dong L, et al. 2021. Cancer Cell. . PubMed
  19. Clarke F,et al. 2017. PLoS One. 10.1371/journal.pone.0186625. PubMed
  20. Zhang Y, et al. 2018. Sci Rep. 8:5994. PubMed
  21. Hsu SPC, et al. 2020. J Neurooncol. 146:417. PubMed
  22. Sadek J, et al. 2021. EMBO Mol Med. 13:e13591. PubMed
  23. Li Q, et al. 2021. Front Immunol. 12:707784. PubMed
  24. Zheng H, et al. 2021. Frontiers in Cell and Developmental Biology. 9:641527. PubMed
  25. De Vlaeminck Y, et al. 2020. Cancers (Basel). 12:. PubMed
  26. Li J, et al. 2021. Front Immunol. 12:649285. PubMed
  27. Krukowski K, et al. 2021. Sci Adv. 7:eabg6702. PubMed
  28. Pajarinen J, et al. 2021. J Biomed Mater Res A. 109:1512. PubMed
  29. Shao H, et al. 2012. J Biol Chem. 287:35860. PubMed
  30. He C, et al. 2021. Oncoimmunology. 1897295:10. PubMed
  31. Tang TT, et al. 2020. Sci Adv. 6:eaaz0748. PubMed
  32. Mul Fedele ML, et al. 2020. Front Cell Infect Microbiol. 10:100. PubMed
  33. El–Achkar GA, et al. 2019. PLoS One. 14:e0216405. PubMed
  34. Zhang J, et al. 2021. J Leukoc Biol. 110:485. PubMed
  35. Tanner L, et al. 2022. Front Pharmacol. 13:899469. PubMed
  36. Hou H, et al. 2021. Front Cell Dev Biol. 9:737003. PubMed
  37. Johansen AZ, et al. 2022. Pharmaceutics. 14:. PubMed
  38. Jia X, et al. 2021. Front Cell Dev Biol. 9:685522. PubMed
  39. Giri J, et al. 2020. Cell Rep. 30:1923. PubMed
  40. Dan H, et al. 2020. Mol Oncol. 14:795. PubMed
  41. Kim JS, et al. 2020. Int J Oncol. 56:1405. PubMed
  42. McKee AM, et al. 2021. iScience. 24:103012. PubMed
  43. Zhang Y, et al. 2022. Nat Commun. 13:4553. PubMed
  44. Rao X, et al. 2022. Cell Death Dis. 13:891. PubMed
  45. Martins L, et al. 2020. Stem Cell Res Ther. 0.786805556. PubMed
  46. Farini A, et al. 2020. Front Physiol. 0.738194444. PubMed
  47. An W et al. 2019. British journal of pharmacology. 176(5):699-710 . PubMed
  48. Li J, et al. 2021. Cell Commun Signal. 19:89. PubMed
  49. Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed
  50. Jiao Y, et al. 2021. Crit Care. 25:356. PubMed
  51. Guérin MV, et al. 2019. Nat Commun. 10:4131. PubMed
  52. Liao T, et al. 2017. Front Immunol. 8:1334. PubMed
  53. Wang J, et al. 2021. Nat Commun. 12:6198. PubMed
  54. Lu X, et al. 2020. Sci Transl Med. 12:. PubMed
  55. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  56. Tacconi C, et al. 2021. Cell Reports. 35(2):108993. PubMed
  57. Lee DH, et al. 2019. PLoS One. 14:e0215727. PubMed
  58. Yu Y, et al. 2018. Mol Med Rep. 17:8212. PubMed
  59. Wei W, et al. 2022. mSystems. 7:e0046922. PubMed
  60. Wang Y, et al. 2022. Cell Death Dis. 13:748. PubMed
  61. Golden TN, et al. 2022. Front Pharmacol. 12:761496. PubMed
  62. Cai C, et al. 2021. Sci Rep. 11:13386. PubMed
  63. Ruiz de Azua I, et al. 2017. J Clin Invest. 127:4148. PubMed
  64. Miura I, et al. 2021. Physiol Rep. 9:e14859. PubMed
  65. Li H, et al. 2021. Nat Commun. 12:7149. PubMed
  66. Germundson DL, et al. 2022. Front Allergy. 3:870513. PubMed
  67. Shu Y, et al. 2020. Immunology. 160:345. PubMed
  68. Ying W, et al. 2016. Sci Rep. 6:20176. PubMed
  69. Sztwiertnia I, et al. 2020. PLoS One. 15:e0233789. PubMed
  70. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  71. Chu SY, et al. 2019. Nat Commun. 10:1524. PubMed
  72. Anderson AE, et al. 2022. NPJ Regen Med. 7:6. PubMed
  73. Lu X, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2358. PubMed
  74. Bhattacharya A, et al. 2018. Neuropsychopharmacology. 43:2586. PubMed
  75. Qiu N, et al. 2021. J Nanobiotechnology. 19:428. PubMed
  76. Yang C, et al. 2022. Nat Commun. 13:4866. PubMed
  77. Lowe PP, et al. 2020. J Neuroinflammation. 0.913888889. PubMed
  78. Zhou K, et al. 2016. J Cereb Blood Flow Metab. 10.1177/0271678X16648712. PubMed
  79. Deng W, et al. 2015. Clin Immunol. . PubMed
  80. Lu SW, et al. 2020. Nat Commun. 3.660416667. PubMed
  81. Anderson-Baucum E, et al. 2021. Cell Metab. 33:1883. PubMed
  82. Stevenson ER, et al. 2022. J Pharmacol Exp Ther. 382:356. PubMed
  83. Wang X, et al. 2022. J Biol Chem. 298:101561. PubMed
  84. Asterholm I, et al. 2012. J Lipid Res. 53: 1254. PubMed
  85. Stridh L, et al. 2013. J Neurosci. 33:12401. PubMed
  86. Long L, et al. 2018. Bio Protoc. 8:e2815. PubMed
  87. Li C, et al. 2019. JCI Insight. 5. PubMed
  88. Budai Z, et al. 2021. Cells. 10:. PubMed
  89. Zhu Q, et al. 2022. Atherosclerosis. 357:41. PubMed
  90. Suzuki T, et al. 2017. Cell Rep. 18(8):2045-2057. PubMed
  91. Krukowski K, et al. 2018. Sci Rep. 8:7857. PubMed
  92. Di Lorenzo A, et al. 2022. Oncoimmunology. 11:2086752. PubMed
  93. Li Y, et al. 2020. Theranostics. 10:2897. PubMed
  94. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
  95. Zhang W, et al. 2021. Front Immunol. 12:768435. PubMed
  96. Santopaolo M, et al. 2021. Front Immunol. 609406:12. PubMed
  97. Ukah T, et al. 2017. J Immunol. 10.4049/jimmunol.1700410. PubMed
  98. Roediger B et al. 2018. Cell. 175(2):530-543 . PubMed
  99. Liu C, et al. 2022. Cell Death Discov. 8:269. PubMed
  100. Sears SM, et al. 2022. Kidney360. 3:818. PubMed
  101. Budhu S, et al. 2021. Cell Reports. 34(2):108620. PubMed
  102. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  103. Browne A, et al. 2015. Cancer Res . 75: 2811 - 2821. PubMed
  104. Moreno M et al. 2014. The Journal of Neuroscience. 34(24):8175-85 . PubMed
  105. Zhang J, et al. 2020. J Cell Mol Med. 24:4547. PubMed
  106. Lau P, et al. 2022. Cell Mol Immunol. :. PubMed
  107. Liu YS, et al. 2021. Cancers (Basel). 13:. PubMed
  108. Sadtler K, et al. 2017. Tissue Eng Part A. 23:1044. PubMed
  109. Li B et al. 2019. Cell reports. 26(10):2720-2737 . PubMed
  110. Morioka S, et al. 2018. Nature. 563:714. PubMed
  111. Mellal K, et al. 2019. Sci Rep. 9:12903. PubMed
  112. Yuan Y, et al. 2019. Clin Sci (Lond). 133:1759. PubMed
  113. Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed
  114. Al-Zaeed N, et al. 2021. Cell Death Dis. 12:611. PubMed
  115. Golabek A, et al. 2021. Exp Ther Med. 21:482. PubMed
  116. Yeini E, et al. 2021. Nat Commun. 12:1912. PubMed
  117. Xu GY, et al. 2022. Small. 18:e2107838. PubMed
  118. Zheng H, et al. 2021. Frontiers in Immunology. 12:645100. PubMed
  119. Chuan Li et al. 2017. Endocrinology. 158(9):2837-2847 . PubMed
  120. Wen HJ, et al. 2019. Cell Mol Gastroenterol Hepatol. 8:p173. PubMed
  121. Yuan M, et al. 2017. Hum Reprod. 32:94. PubMed
  122. Riopel M, et al. 2019. Mol Metab. 20:89. PubMed
  123. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
  124. Cai T, et al. 2022. Front Immunol. 13:1009932. PubMed
  125. Lal JC, et al. 2021. Breast Cancer Res. 23:83. PubMed
  126. Guo S, et al. 2021. Chronic Dis Transl Med. 7:254. PubMed
RRID
AB_10896421 (BioLegend Cat. No. 141705)
AB_10895754 (BioLegend Cat. No. 141706)

Antigen Details

Structure
Type I transmembrane protein, 175 kD, C-type lectin superfamily
Distribution

Macrophages, dendritic cells, Langerhans cells, liver endothelial cells

Function
Pathogen recognition, endocytosis and phagocytosis, antigen presentation
Ligand/Receptor
Antigen containing mannose, fucose, or an N-acetyl glucosamine
Cell Type
Dendritic cells, Endothelial cells, Langerhans cells, Macrophages
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
CD Molecules
Antigen References

1. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501.
2. Apostolopoulos V, et al. 2001. Curr. Mol. Med. 1:469.
3. Burgdorf S, et al. 2006. J. Immunol. 176:6770.
4. McKenzie EJ, et al. 2007. J. Immunol. 178:4975.

Gene ID
17533 View all products for this Gene ID
UniProt
View information about CD206 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Why is mouse CD206 stained intracellularly and not via surface staining?

Typically, mouse CD206 surface level is relatively low under normal conditions and so intracellular staining protocol is required to get better signal.

Go To Top Version: 3    Revision Date: 04/04/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account