Loading...

PE anti-mouse CD11c Antibody

Pricing & Availability
Clone
N418 (See other available formats)
Regulatory Status
RUO
Other Names
αX integrin, integrin αX chain, CR4, p150, ITGAX
Isotype
Armenian Hamster IgG
Ave. Rating
Submit a Review
Product Citations
publications
N418_A_PE_Pos_071006.jpg
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and PE N418 (top) or PE Armenian hamster IgG isotype control (bottom)
  • N418_A_PE_Pos_071006.jpg
    C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and PE N418 (top) or PE Armenian hamster IgG isotype control (bottom)
  • N418_B_PE_Neg_071006.jpg
    C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and PE N418 (top) or PE Armenian hamster IgG isotype control (bottom)
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
117307 50 µg 58€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
117308 200 µg 147€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD11c is a 150 kD glycoprotein also known as αX integrin, CR4, and p150. CD11c forms a αXβ2 heterodimer with β2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The αXβ2 integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen, and CD54.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse spleen dendritic cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD11c antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for other applications.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, immunofluorescence microscopy5, 9 (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10), and spatial biology (IBEX)22,23.

Application References
  1. Granucci F, et al. 1997. J. Immunol. 159:1794.
  2. Stokes RW, et al. 1998. J. Immunol. 160:5514.
  3. Metlay JP, et al. 1990. J. Exp. Med. 171:1753. (IHC, IP)
  4. Ma XT, et al. 2006. Cancer Research 66:1169.
  5. Chin RK, et al. 2006. J. Immunol. 177:290. (IF)
  6. Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) PubMed
  7. Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) PubMed
  8. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  9. You Y, et al. 2009. J. Immunol. 182:7343. (IF) PubMed
  10. Roland CL, et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed
  11. Wikstrom M, et al.2006. J. Immunol. 177:913. PubMed
  12. Pericolini E, et al. 2008. J. Leukocyte Biol. 83:1286. PubMed
  13. Randall LM, et al. 2008. Infect. Immun.76:3312. PubMed
  14. Fahlen-Yrild L, et al. 2009. J. Immunol. 183:5032. PubMed
  15. Osterholzer JJ, et al. 2009. J. Immunol. 183:8044. PubMed
  16. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  17. Eisenach PA, et al. 2010. J Cell Sci. 123:4182. PubMed
  18. Leppin K, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:3603. PubMed
  19. Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
  20. Gibbins JD, et al. 2014. Blood. 124:2953. PubMed
  21. White CE, et al. 2015. J Immunol. 194:697. PubMed
  22. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  23. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  24. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  2. Lin C et al. 2019. Immunity. 50(1):137-151 . PubMed
  3. Xiong Y, et al. 2020. J Nanobiotechnology. 18:66. PubMed
  4. Zhao Z, et al. 2021. Nat Commun. 12:4355. PubMed
  5. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  6. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  7. Liang J, et al. 2021. Cancer Manag Res. 13:6977. PubMed
  8. Lee T, et al. 2014. Mol Biol Cell. 25:583. PubMed
  9. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  10. Tseng K, et al. 2009. J Immunol. 182:4590. PubMed
  11. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  12. Lee S, et al. 2021. JCI Insight. 6:. PubMed
  13. Fan L, et al. 2021. Cancers (Basel). 13:. PubMed
  14. Zhang R, et al. 2021. Cell Mol Immunol. 18:1222. PubMed
  15. Du J, et al. 2020. Developmental Cell. 55(6):737-753.e7. PubMed
  16. Naito Y, et al. 2019. American Journal of Physiology-Renal Physiology. 318(1):F238-F247. PubMed
  17. Jackson A, et al. 2014. J Leukoc Biol. 92:609. PubMed
  18. Blecher-Gonen R, et al. 2019. Cell Syst. 8:109. PubMed
  19. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  20. Lee H, et al. 2020. Cell Metabolism. 31(4):822-836. PubMed
  21. Kunimura K, et al. 2020. Cell Reports. 29(9):2823-2834.e7.. PubMed
  22. Zhang J, et al. 2021. MedComm (Beijing). 2:256. PubMed
  23. Kang S, et al. 2009. Gastroenterology. 137:1391. PubMed
  24. Clark K, et al. 2015. J Autoimmun. Available online 21 August 2015. PubMed
  25. Wu R, et al. 2019. J Cell Mol Med. 24:1684. PubMed
  26. Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
  27. Shu T, et al. 2022. Eur Respir J. Online ahead of print. PubMed
  28. Cheong SS, et al. 2020. Front Cell Dev Biol. 8:577201. PubMed
  29. Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
  30. Nakagawa S et al. 2017. Cell host & microbe. 22(5):667-677 . PubMed
  31. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  32. Lees JG, et al. 2020. PLoS One. 15:e0238164. PubMed
  33. Xiong A, et al. 2022. EBioMedicine. 83:104239. PubMed
  34. Hu K, et al. 2015. PLoS One. 10: 0137123. PubMed
  35. Toivonen R, et al. 2016. J Immunol. 196: 4750 - 4759. PubMed
  36. Shemer A, et al. 2020. Immunity. 53(5):1033-1049.e7. PubMed
  37. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  38. Privratsky JR, et al. 2018. Am J Physiol Renal Physiol. 315:F682. PubMed
  39. Akram KM, et al. 2019. Nat Commun. 10:1178. PubMed
  40. Tran L, et al. 2017. Cancer Immunol Res. 5:1141. PubMed
  41. Wang C, et al. 2017. J Immunol. 10.4049/jimmunol.1700671. PubMed
  42. Li X, et al. 2015. J Immunol. 195: 2710-2721. PubMed
  43. Fujita K, et al. 2019. Proc Natl Acad Sci U S A. 116:14714. PubMed
  44. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  45. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  46. Dane EL, et al. 2022. Nat Mater. 21:710. PubMed
  47. Syed A, et al. 2015. Infect Immun . 83: 3428-3437. PubMed
  48. Ma Y, et al. 2015. J Immunol. 195: 3769 - 3780. PubMed
  49. Chi Z, et al. 2020. Molecular Cell. 80(1):43-58.e7. PubMed
  50. Fulham MA, et al. 2019. Am J Physiol Cell Physiol. 317:C687. PubMed
  51. Benechet AP, et al. 2019. Nature. 574:200. PubMed
  52. Feng T, et al. 2022. Nat Commun. 13:5208. PubMed
  53. Zhang L, et al. 2021. J Immunother Cancer. 9:. PubMed
  54. Li Z, et al. 2022. J Exp Clin Cancer Res. 41:74. PubMed
  55. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  56. Jiao Y, et al. 2021. Crit Care. 25:356. PubMed
  57. Jeong SH, et al. 2021. Nat Commun. 12:4405. PubMed
  58. Carozza JA, et al. 2020. Nat Cancer. 184:1. PubMed
  59. Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed
  60. Galvani E, et al. 2020. Nat Commun. 11:853. PubMed
  61. Garcia LR, et al. 2021. Nat Commun. 12:3364. PubMed
  62. Park SG, et al. 2021. J Vet Sci. 22:e16. PubMed
  63. Wang J, et al. 2021. Nat Commun. 12:6198. PubMed
  64. Friedman DJ, et al. 2021. Cancer Immunol Res. 9:952. PubMed
  65. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  66. Guo Y, et al. 2022. Nat Commun. 13:6534. PubMed
  67. Ray A, et al. 2015. PLoS One. 10: 0137314. PubMed
  68. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  69. Garo LP, et al. 2019. Cell Rep. 28:3353. PubMed
  70. Miteva K, et al. 2018. Sci Rep. 8:2820. PubMed
  71. Ding Y, et al. 2022. Cell Death Dis. 13:996. PubMed
  72. Yong L, et al. 2022. Nat Commun. 13:4255. PubMed
  73. Pilones KA, et al. 2020. Cancer Immunol Res. 8:1054. PubMed
  74. Reinfeld BI, et al. 2021. Nature. 593:282. PubMed
  75. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  76. Barsoumian HB, et al. 2020. J Immunother Cancer. 8:00. PubMed
  77. Osaka M, et al. 2016. Sci Rep. 6:21391. PubMed
  78. Van Winkle JA et al. 2018. Cell host & microbe. 24(5):665-676 . PubMed
  79. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  80. Yang H, et al. 2019. J Neuroinflammation. 16:169. PubMed
  81. Liu S, et al. 2020. Cell Host & Microbe. 26(6):779-794.e8.. PubMed
  82. Vorselen D, et al. 2021. Elife. 10:. PubMed
  83. Dey S, et al. 2020. J Immunother Cancer. 8:. PubMed
  84. Olson M, et al. 2014. J Immunol. 193:5420. PubMed
  85. Zeng W, et al. 2021. STAR Protocols. 2(1):100361. PubMed
  86. De Simone G, et al. 2021. Immunity. :. PubMed
  87. Lim CX, et al. 2020. Cell Rep. 3793:30. PubMed
  88. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
  89. López MJ, et al. 2018. Invest Ophthalmol Vis Sci. 59:5671. PubMed
  90. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  91. Maulloo CD, et al. 2021. Front Immunol. 12:714842. PubMed
  92. Koikawa K, et al. 2021. Cell. 184:4753. PubMed
  93. Hanihara-Tatsuzawa F, et al. 2014. J Biol Chem. 389:30925. PubMed
  94. Hsu Y, et al. 2017. Biochem Biophys Res Commun.. 10.1016/j.bbrc.2017.10.067. PubMed
  95. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  96. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
  97. Hu Y, et al. 2021. Cell Death Dis. 12:743. PubMed
  98. Li D, et al. 2022. Emerg Microbes Infect. 11:2248. PubMed
  99. Benhamron S, et al. 2012. PLoS One. 7:e35602. PubMed
  100. Rosenbaum SR, et al. 2020. Cell Rep. 30:510. PubMed
  101. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  102. Wang Y, et al. 2021. Nat Commun. 12:4964. PubMed
  103. Takahashi F, et al. 2022. iScience. 25:104278. PubMed
  104. Wang W, et al. 2021. J Am Heart Assoc. 10:e019142. PubMed
  105. Nahrendorf W, et al. 2021. eLife. 10:00. PubMed
  106. Bi CS, et al. 2020. Cell Prolif. 53:e12827. PubMed
  107. Nechama M, et al. 2018. Nat Commun. 9:1603. PubMed
  108. Xia Y, et al. 2018. Cell. 175:1059. PubMed
  109. Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed
  110. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  111. Lu X, et al. 2019. Circ Res. 125:1055. PubMed
  112. Zheng B, et al. 2021. Bioact Mater. 6:3879. PubMed
  113. Zhang MS, et al. 2022. Nat Commun. 13:954. PubMed
  114. Tuong ZK, et al. 2021. Cell Rep. 37:110132. PubMed
  115. Kataru RP, et al. 2022. Front Aging. 3:864860. PubMed
  116. Al-Barwani F, et al. 2014. PLoS One. 9:104523. PubMed
  117. Holzki J, et al. 2015. J Virol. 89: 9886 - 9895. PubMed
  118. Gamradt P, et al. 2016. PLoS Pathog. 12:e1006032. PubMed
  119. Younes AI, et al. 2021. Transl Oncol. 14:100983. PubMed
  120. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  121. Godwin MS, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 320:L393. PubMed
  122. Gu H, et al. 2021. Elife. 10:. PubMed
  123. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  124. Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed
  125. Yuan C, et al. 2015. Biochem Biophys Res Commun. 464: 249-255. PubMed
  126. Bussey KA, et al. 2019. J Virol. 93. PubMed
  127. Kataru RP, et al. 2019. Cancer Immunol Res. 7:1345. PubMed
  128. Melgert B, et al. 2010. Am J Respir Cell Mol Biol. 42:595. PubMed
  129. Park C, et al. 2012. J Leukoc Biol. 91:739. PubMed
  130. Parodi B, et al. 2021. Front Immunol. 12:655212. PubMed
  131. Fujimura N, et al. 2016. Sci Rep. 5:11664. PubMed
  132. Manifold-Wheeler B, et al. 2016. J Immunol. 196: 328 - 335. PubMed
  133. Zhou H, et al. 2016. J Immunol. 197: 288 - 295. PubMed
  134. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  135. Lai JH, et al. 2021. iScience. 24(6):102498. PubMed
  136. Sharma M, et al. 2019. Immunometabolism. 1. PubMed
  137. Zukauskas A, et al. 2018. mSphere. 3:e00303. PubMed
  138. Hao L, et al. 2021. Br J Pharmacol. 178:4726. PubMed
  139. Tomita T, et al. 2021. Nat Commun. 12:3655. PubMed
  140. Siolas D, et al. 2021. Cell Reports. 36:109578. PubMed
  141. Iwai H, et al. 2015. Tuberculosis (Edinb). 95:246. PubMed
  142. Li A, et al. 2018. Cancer Lett. 431:54. PubMed
  143. Mino T, et al. 2019. Nucleic Acids Res. 47:8838. PubMed
  144. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  145. Jones GS, et al. 2020. mSphere. 5:. PubMed
  146. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  147. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  148. Wu L, et al. 2020. Cancer Immunol Res. 710:8. PubMed
  149. Tian T, et al. 2020. Cancer Immunol Res. 660:8. PubMed
  150. Mangal JL, et al. 2020. J Mater Chem B. 8:5195. PubMed
  151. Vecchiola A, et al. 2020. Front Endocrinol (Lausanne). 11:223. PubMed
  152. Giles DA, et al. 2018. J Clin Invest. 128:5322. PubMed
  153. Yamamoto R, et al. 2019. J Immunol. 203:167. PubMed
  154. Omenetti S, et al. 2019. Immunity. 51:77. PubMed
  155. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
  156. Syed I et al. 2018. Cell metabolism. 27(2):419-427 . PubMed
  157. Li CY, et al. 2022. Int J Mol Sci. 23:. PubMed
  158. Li C, et al. 2022. Front Cell Dev Biol. 10:913824. PubMed
  159. Zhong J, et al. 2021. Commun Biol. 4:865. PubMed
  160. Chow AK, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. :. PubMed
  161. Chng S, et al. 2016. Sci Rep. 6: 23820. PubMed
  162. Huanga J, et al. 2013. J Immunol Methods. 387:254. PubMed
RRID
AB_313776 (BioLegend Cat. No. 117307)
AB_313777 (BioLegend Cat. No. 117308)

Antigen Details

Structure
Integrin α-chain, associates with integrin β2 (CD18), 150 kD
Distribution

Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells

Function
Cellular adhesion
Ligand/Receptor
iC3b, fibrinogen
Cell Type
Dendritic cells, Epithelial cells, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780.

Gene ID
16411 View all products for this Gene ID
UniProt
View information about CD11c on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All CD11c Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD11c N418 FC
Biotin anti-mouse CD11c N418 FC,IHC-F
FITC anti-mouse CD11c N418 FC
PE anti-mouse CD11c N418 FC
Purified anti-mouse CD11c N418 FC,CyTOF®,IP,IHC
Alexa Fluor® 488 anti-mouse CD11c N418 FC
Alexa Fluor® 647 anti-mouse CD11c N418 FC,IHC,ICC,3D IHC
PE/Cyanine5 anti-mouse CD11c N418 FC
PE/Cyanine7 anti-mouse CD11c N418 FC
Brilliant Violet 605™ anti-mouse CD11c N418 FC
Alexa Fluor® 700 anti-mouse CD11c N418 FC
Pacific Blue™ anti-mouse CD11c N418 FC
APC/Cyanine7 anti-mouse CD11c N418 FC
PerCP/Cyanine5.5 anti-mouse CD11c N418 FC
PerCP anti-mouse CD11c N418 FC
Brilliant Violet 421™ anti-mouse CD11c N418 FC,IHC
Brilliant Violet 570™ anti-mouse CD11c N418 FC
Brilliant Violet 785™ anti-mouse CD11c N418 FC
Brilliant Violet 510™ anti-mouse CD11c N418 FC
Brilliant Violet 650™ anti-mouse CD11c N418 FC
Purified anti-mouse CD11c (Maxpar® Ready) N418 FC,CyTOF®
Alexa Fluor® 594 anti-mouse CD11c N418 IHC-F,3D IHC
PE/Dazzle™ 594 anti-mouse CD11c N418 FC
Brilliant Violet 711™ anti-mouse CD11c N418 FC
APC/Fire™ 750 anti-mouse CD11c N418 FC
TotalSeq™-A0106 anti-mouse CD11c N418 PG
Brilliant Violet 750™ anti-mouse CD11c N418 FC
TotalSeq™-B0106 anti-mouse CD11c N418 PG
TotalSeq™-C0106 anti-mouse CD11c N418 PG
KIRAVIA Blue 520™ anti-mouse CD11c N418 FC
Spark Blue™ 550 anti-mouse CD11c N418 FC
Spark NIR™ 685 anti-mouse CD11c N418 FC
Spark UV™ 387 anti-mouse CD11c N418 FC
Spark Red™ 718 anti-mouse CD11c N418 FC
Spark Blue™ 515 anti-mouse CD11c N418 FC
PerCP/Fire™ 806 anti-mouse CD11c N418 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account