PE anti-mouse CD115 (CSF-1R) Antibody

Pricing & Availability
Clone
AFS98 (See other available formats)
Other Names
CSF-1R, M-CSFR, c-fms
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A_AFS98_PE_102309
Thioglycolate-elicited BALB/c mouse peritoneal macrophages stained with rat IgG1, k PE and CD11b FITC.
  • A_AFS98_PE_102309
    Thioglycolate-elicited BALB/c mouse peritoneal macrophages stained with rat IgG1, k PE and CD11b FITC.
  • B_AFS98_PE_102309
    Thioglycolate-elicited BALB/c mouse peritoneal macrophages stained with AFS98 PE and CD11b FITC.
See PE spectral data
Cat # Size Price Quantity Avail. Save
135505 50 µg 76€
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135506 200 µg 236€
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Description

CSF-1R, also known as CD115 and M-CSFR, is a single-pass type I membrane protein and member of the platelet-derived growth factor receptor family. This c-fms (Fms proto-oncogene) gene product's natural ligands include M-CSF and IL-34. Structural studies of CD115 have described an Ig-like extracellular domain, a transmembrane domain, an intracellular juxtamembrane domain, a split tyrosine kinase domain, and a C-terminal tail receptor. Receptor activation induces homodimerization in addition to phosphorylation and ubiquitination of intracellular residues. CD115 directly influences tissue macrophage and osteoclast differentiation and proliferation. It is expressed on monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, and osteoclasts.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of ligand binding1. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.

It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation. Pre-treatment with EDTA and low temperatures (2 to 8°C) helps in maintaining surface expression of CD1154.

Application References
  1. Sudo T, et al. 1995. Oncogene 11:2469.
  2. Murayama T, et al. 1999. Circulation 99:1740.
  3. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. PubMed
  4. Breslin WL, et al. 2013. J Immunol Methods. 390(1-2):1 PubMed
  5. Dong L, et al. 2016. Nature. 539:304–308. PubMed
Product Citations
  1. Clemente C, et al. 2018. Nat Commun. 9:910. PubMed
  2. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  3. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  4. Di Liberto G et al. 2018. Cell. 175(2):458-471 . PubMed
  5. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  6. Combes F, et al. 2018. Neoplasia. 20:848. PubMed
  7. Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed
  8. Petriello MC, et al. 2018. Toxicol Sci. 162:548. PubMed
  9. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  10. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  11. Barman PK, et al. 2019. J Immunol. 202:2720. PubMed
  12. Anderson SR, et al. 2019. Cell Rep. 27:2002. PubMed
  13. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  14. Sierro F, et al. 2017. Immunity. 47:374. PubMed
  15. Burgener SS, et al. 2019. Cell Rep. 27:3646. PubMed
  16. Takacs E, et al. 2017. J Immunol. 198:239. PubMed
  17. Lee MH, et al. 2018. J Immunol Res. 2018:1601079. PubMed
  18. Garcia–Agudo LF, et al. 2019. FASEB J. :fj201900337R. PubMed
  19. Sakai M, et al. 2020. Immunity. 51(4):655-670. PubMed
  20. Bouchareychas L, et al. 2020. Cell Reports. 32(2):107881. PubMed
  21. Jain A, et al. 2020. Nat Immunol. 0.920138889. PubMed
  22. Smith C, et al. 2012. Cancer Discovery. 2:722. PubMed
  23. Ryu J, et al. 2013. Circulation. 127:710. PubMed
  24. Johansson J, et al. 2013. J Neurosci. 33:16016. PubMed
  25. Jackson A, et al. 2014. J Leukoc Biol. 92:609. PubMed
  26. Chen S, et al. 2014. Biochem Biophys Res Commun. 446:1002. PubMed
  27. Distel E, et al. 2014. Circ Res . 115:759. PubMed
  28. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  29. Oliveira-Lima OC, et al. 2015. Immunobiology. . PubMed
  30. Hussein M, et al. 2015. PLoS One. 10: 0135218. PubMed
  31. Mairhofer D, et al. 2015. J Invest Dermatol. 135: 2785-93. PubMed
  32. Foster G, et al. 2015. J Immunol. 195: 5380 - 5392. PubMed
  33. Steinmetz O, et al. 2016. J Infect Dis. 10.1093/infdis/jiv763. PubMed
  34. Gadd V, et al. 2016. PLoS One. 11: 0157771. PubMed
  35. Ulrich V, et al. 2016. EMBO Mol Med. 8: 643 - 653. PubMed
  36. Aryal B, et al. 2016. Nat Commun. 7:12313. PubMed
  37. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
RRID
AB_1937254 (BioLegend Cat. No. 135505)
AB_1937253 (BioLegend Cat. No. 135506)

Antigen Details

Structure
Ig superfamily, 145 kD
Distribution

Monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, osteoclasts

Function
Growth factor receptor, tyrosine kinase
Ligand/Receptor
Macrophage colony stimulating factor (M-CSF), IL-34
Cell Type
Dendritic cells, Macrophages, Monocytes, Osteoclasts
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Sudo T, et al. 1995 Oncogene 11:2469.
  2. Murayama T, et al. 1999 Circulation 99:1740.
  3. Goswami S, et al. 2005 Cancer Res. 65:5278.
  4. Yu W, et al. 2008 J. Leuko. Biol. 84:852.
Gene ID
12978 View all products for this Gene ID
UniProt
View information about CD115 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Why do I have a weak CD115 staining?

It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation.  Pre-treatment with EDTA and low temperatures (2 - 8°C) helps in maintaining surface expression of CD115. In addition, brief fixation of the cells with Fixation Buffer (Cat. No. 420801) for 10 minutes will block CD115 internalization.

Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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