PE anti-human TNF-α, TNF-alpha, MAb11

Pricing & Availability

Analyte Specific Reagent. Analytical and performance characteristics are not established.

Clone: MAb11
Other Names: Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2
Isotype: Mouse IgG1, κ

MAb11_PE_TNF-alpha_ASR_020323 — PMA + Ionomycin with Brefeldin A stimulated (4-hour) human peripheral blood lymphocytes were fixed with Fixation Buffer (Cat# 420801), permeabilized with Permeabilization Wash Buffer (Cat# 421002), and intracellularly stained stained either with MAb11 PE used at 5 µL/test (filled histogram) or with an isotype control (open histogram).

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Size

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986802

500 µL

258€

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Description

TNF-α is secreted by macrophages, monocytes, neutrophils, T cells, and NK cells. Many transformed cell lines also secrete TNF-α. Monomeric human TNF-α is a 157 amino acid protein (non-glycosylated) with a reported molecular weight of 17 kD. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biological activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.

Product Details

Technical Data Sheet (pdf)

Product Details

Reactivity

Human

Formulation

Phosphate-buffered solution, pH7.2, 0.09% sodium azide, 0.2% (w/v) BSA (origin USA), and a stabilizer.

Preparation

The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.

Concentration

100 µg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

Suggested for Intracellular Staining for Flow Cytometry

Disclaimer

WARNINGS AND PRECAUTIONS

Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal.
All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
Do not use this reagent beyond the expiration date stated on the label.
Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be an indication of possible deterioration.
Avoid prolonged exposure of the reagent or stained cells to light.

Antigen Details

Antigen References

Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
Beutler B, et al. 1988. Annu Rev Biochem. 57:505-18.
Beutler B, et al. 1989. Annu Rev Immunol. 7:625-55.
Tracey K, et al. 1993. Crit Care Med. 21:S415-22.

Documentation

Certificate of Analysis

Go To Top Version: 2 Revision Date: 03/06/2023

Analyte Specific Reagent. Analytical and performance characteristic are not established.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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