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PE anti-human CD41/CD61 Antibody

Pricing & Availability
Clone
A2A9/6 (See other available formats)
Regulatory Status
RUO
Other Names
gpIIb/gpIIIa
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A29A9_PE_Antibody_CD41_CD61_Antibody_FC_102813
Human peripheral blood platelets were stained with CD41/CD61 (clone A2A9/6) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).
  • A29A9_PE_Antibody_CD41_CD61_Antibody_FC_102813
    Human peripheral blood platelets were stained with CD41/CD61 (clone A2A9/6) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).
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359805 25 tests 84€
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359806 100 tests 220€
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Description

CD41/CD61, also known as gpIIb/IIIa, is a member of a family integrin receptors. This is a complex comprised by CD41 and CD61 through non-covalent association. CD41/CD61 is mainly expressed by platelets and megakaryocytes. The resting form of the CD41/CD61 complex is involved in platelet activation and aggregation by binding to immobilized fibrinogen. After activation, CD41/CD61 becomes a receptor for soluble fibrinogen and several other RGD-containing adhesive proteins such as von Willebrand Factor (vWF) and fibronectin. An absence or dysfunction of CD41/CD61 on the platelet surface results in an inherited bleeding disorder, called Glanzmann Thromsasthenia (GT). CD41/CD61 has been found on murine hematopoietic progenitor cells, indicating that this complex may play a role in regulating hematopoietic development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human platelets
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation1 and blocking (aggregation)2. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 359804).

A2A9/6 is recognized as having high affinity to gpIIb/IIIa. It has been shown to inhibit platelet aggregation induced by a variety of agonists and the initiation of clot formation.

Application References
  1. Basani RB, et al. 1996. Blood. 88:167. (IP)
  2. Bennett JS, et al. 1983. Proc. Natl. Acad. Sci. USA. 80:2417. (Block)
Product Citations
  1. Slingsby MHL, et al. 2021. Haematologica. Online ahead of print. PubMed
  2. Marsden AJ, et al. 2021. ACS Pharmacol Transl Sci. 4:1567. PubMed
RRID
AB_2562700 (BioLegend Cat. No. 359805)
AB_2562701 (BioLegend Cat. No. 359806)

Antigen Details

Structure
Complex of CD41 and CD61 through non-covalent association.
Distribution

Platelets and megakaryocytes.

Function
Mediates attachment to cells of diverse matrix proteins.
Ligand/Receptor
Fibrinogen, RGD-containing adhesive proteins such as vonWillebrand Factor (vWF) and fibronectin.
Cell Type
Megakaryocytes, Platelets
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Matsumura-Takeda K, et al. 2007. Stem Cell. 25:862.
2. Corbel C, et al. 2005. 49:279.
3. Bennett JS, et al. 1983. Proc. Natl. Acad. Sci. USA. 80:2417. 
4. Clemetson KJ, et al. 1994. Curr. Opin. Hematol. 1:388.

Gene ID
3690 View all products for this Gene ID 3674 View all products for this Gene ID
UniProt
View information about CD41/CD61 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 10/31/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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