PE anti-human CD274 (B7-H1, PD-L1) Antibody

Pricing & Availability
Clone
29E.2A3 (See other available formats)
Regulatory Status
RUO
Other Names
Programmed cell death ligand 1 (PD-L1), B7 homolog 1 (B7-H1)
Isotype
Mouse IgG2b, κ
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Product Citations
publications
29E.2A3_PE_CD274_Antibody_FC_091013
PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with CD274 (clone 29E.2A3) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).
  • 29E.2A3_PE_CD274_Antibody_FC_091013
    PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with CD274 (clone 29E.2A3) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).
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329705 25 tests 95€
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329706 100 tests 211€
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Description

CD274, also known as PD-L1 and B7-H1, is type I transmembrane glycoprotein that serves as a ligand for CD279 (PD-1). This interaction is believed to regulate the balance between the stimulatory and inhibitory signals needed for responses to microbes and maintenance of self-tolerance. CD274 is involved in the costimulation of T cell proliferation and IL-10 and IFN-γ production in an IL-2-dependent and CD279-independent manner. Conflicting data has shown that CD274 can inhibit T cell proliferation and cytokine production, and alternatively, enhance T cell activation. Other studies suggest that CD274 may signal bidirectionally, raising interesting implications for its expression in a wide variety of cell types, including T and B cells, antigen-presenting cells, and nonhematopoietic cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length human PD-L1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone 29E.2A3 is reported to recognize an epitope on PD-L1 within the PD-L1-CD80 binding region5. Additional reported applications (for the relevant formats) include: blocking1-3 and immunohistochemical staining of acetone-fixed frozen sections1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329715, 329716, 329745 - 329748). 

It has been observed that clone 29E.2A3 is able to bind to Alexa Fluor® 700 antibody conjugates during multi-color immunofluorescent staining. This interaction can be resolved by sequentially staining with the 29E.2A3 antibody first and then followed by the Alexa Fluor® 700 conjugate of interest.

Clone 29E.2A3 does not work in Western blot applications7.

Application References
  1. Brown J, et al. 2003. J. Immunol. 170:1257. (FC, IHC, Block)
  2. Radziewicz H, et al. 2007. J. Virol. 81:2545. (Block)
  3. Nakamoto N, et al. 2009. PLoS Pathog. 5:e1000313. (Block)
  4. Barsoum IB, et al. 2014. Cancer Res. 74:665. PubMed
  5. Haile, S et al. 2013. J. Immunol. 191:2829.
  6. RL M, et al. 2015. PNAS. 112:6506-6514. PubMed
  7. Mahoney KM, et al. 2015. Cancer Immunol. Res. 3:1308.
Product Citations
  1. Jiang G, et al. 2020. Aging (Albany NY). 12:11466. PubMed
  2. Shao L, et al. 2021. Cell Death Discov. 7:145. PubMed
  3. Cui J, et al. 2022. Cancer Cell Int. 22:72. PubMed
  4. Oreskovic E, et al. 2022. Proc Natl Acad Sci U S A. 119:. PubMed
  5. Soday L, et al. 2021. Frontiers in Immunology. 12:600056. PubMed
  6. Schott DS, et al. 2017. Oncotarget. 8:72755. PubMed
  7. Roberts A, et al. 2021. Sci Rep. 11:4030. PubMed
  8. Katsurahara K, et al. 2021. Cancer Sci. 112:1026. PubMed
  9. Geuijen C, et al. 2021. Nat Commun. 12:4445. PubMed
  10. Kagoya Y, et al. 2018. Nat Commun. 9:1915. PubMed
  11. Sucker A, et al. 2017. Nat Commun. 8:15440. PubMed
  12. Huang X, et al. 2021. Nat Nanotechnol. 16:214. PubMed
  13. Zhang H, et al. 2021. Cancer Gene Ther. Online ahead of print. PubMed
  14. Jarosch S, et al. 2022. STAR Protoc. 3:101374. PubMed
  15. Liu Y, et al. 2021. Oncogene. 40:2230. PubMed
  16. Sugawara E, et al. 2020. Autophagy. 1.323611111. PubMed
  17. Rosskopf S, et al. 2016. Sci Rep. 6:31580. PubMed
  18. Fujiwara Y, et al. 2021. Pancreas. 50:405. PubMed
  19. Yi K, et al. 2022. Front Immunol. 12:802795. PubMed
  20. Tran L, et al. 2017. Cancer Immunol Res. 5:1141. PubMed
  21. Mineo M, et al. 2020. Molecular Cell. 78(6):1207-1223.e8. PubMed
  22. Guan H, et al. 2016. Sci Rep. 6:35651. PubMed
  23. Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
  24. Zong Z, et al. 2019. Front Immunol. 10:1643. PubMed
  25. Herter JM, et al. 2022. Strahlenther Onkol. Online ahead of print. PubMed
  26. Nduom E, et al. 2016. Neuro Oncology. 18: 195 - 205. PubMed
  27. Bryson BD, et al. 2019. Nat Commun. 10:2329. PubMed
  28. Lee JM, et al. 2022. Lupus Sci Med. 9:. PubMed
  29. Leonard NA, et al. 2021. Cancers (Basel). 13:. PubMed
  30. Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
  31. Rolfes V, et al. 2018. Oncotarget. 9:27460. PubMed
  32. Souriant S, et al. 2019. Cell Rep. 26:3586. PubMed
  33. Wu Y, et al. 2021. Nat Commun. 12:2346. PubMed
  34. Wang Y, et al. 2021. Front Immunol. 12:654463. PubMed
  35. El Ahanidi H, et al. 2022. Front Oncol. 11:795242. PubMed
  36. Jarosch S, et al. 2021. Cell Rep Methods. 1:100104. PubMed
  37. Fan Z, et al. 2020. EMBO Mol Med. 12:e11571. PubMed
  38. Wu L, et al. 2018. Oncol Lett. 15:9507. PubMed
  39. Hu H, et al. 2022. Exp Ther Med. 24:487. PubMed
  40. Zhang R, et al. 2021. Front Oncol. 10:581733. PubMed
  41. Mao R, et al. 2020. Cancer Sci. 111:3174. PubMed
  42. Durand-Panteix S, et al. 2012. J Immunol. 189:181. PubMed
  43. Su S et al. 2018. Cell. 175(2):442-457 . PubMed
  44. Bouleau A, et al. 2022. J Nucl Med. 63:1259. PubMed
  45. Pathania AS, et al. 2022. Mol Ther Oncolytics. 25:308. PubMed
  46. Houtsma R, et al. 2021. STAR Protoc. 2:100864. PubMed
  47. Okita R, et al. 2021. Thorac Cancer. 12:775. PubMed
  48. Li H, et al. 2021. Adv Sci (Weinh). 2001596:8. PubMed
  49. Ye LL, et al. 2020. Annals of Translational Medicine. 8(24):1647. PubMed
  50. Iwasa M, et al. 2019. Oncotarget. 10:1903. PubMed
  51. Richardson JR, et al. 2018. Front Immunol. 2.182638889. PubMed
  52. Yoshino H, et al. 2021. Curr Issues Mol Biol. 43:153. PubMed
  53. Kim DH, et al. 2019. Exp Mol Med. 51:94. PubMed
  54. Zhao Y et al. 2018. Cell reports. 24(2):379-390 . PubMed
  55. Curnock AP, et al. 2021. JCI Insight. 6:. PubMed
  56. Rydyznski Moderbacher C, et al. 2020. Cell. 183(4):996-1012.e19. PubMed
  57. Zhou C, et al. 2021. Mol Ther. 29:1512. PubMed
  58. Ahn A, et al. 2021. Cancers (Basel). 13:. PubMed
  59. Tsuchiya K, et al. 2021. Oncoimmunology. 10:1962656. PubMed
  60. Wang J, et al. 2022. Clin Transl Med. 12:e718. PubMed
  61. Rydyznski Moderbacher C, et al. 2022. J Clin Invest. :. PubMed
  62. Guo Q, et al. 2022. Blood Adv. 6:5668. PubMed
  63. Zhang Z, et al. 2022. Clin Transl Med. 12:e1072. PubMed
  64. Dan J, et al. 2016. J Immunol. 197: 983 - 993. PubMed
  65. Lundell A, et al. 2017. Sci Rep. 7:39904. PubMed
  66. Park JA, et al. 2020. J Hematol Oncol. 0.661111111. PubMed
  67. Kwak T, et al. 2020. Cell Reports. 33(13):108571. PubMed
  68. Lei Y, et al. 2021. Clin Transl Immunology. 10:e1231. PubMed
  69. Ng KW, et al. 2019. eLife. 0.333333333333333. PubMed
  70. Xu H, et al. 2010. J Immunol. 185:7340. PubMed
  71. Sam J, et al. 2020. Front Oncol. 10:575737. PubMed
  72. Dai X, et al. 2021. Molecular Cell. 81(11):2317-2331.e6. PubMed
  73. Gilardini Montani MS, et al. 2020. Br J Cancer. 123:298. PubMed
  74. Suzuki D, et al. 2020. Stem Cell Reports. 14:49. PubMed
  75. Zhou X, et al. 2022. J Nucl Med. 63:536. PubMed
  76. Hu Q, et al. 2021. Nat Biomed Eng. 5:1038. PubMed
  77. Lau P, et al. 2022. Cell Mol Immunol. :. PubMed
  78. Darga EP, et al. 2021. PLoS One. 16:e0260124. PubMed
  79. Zhang H, et al. 2021. Adv Sci (Weinh). 8:2003404. PubMed
  80. Padet L, et al. 2014. Immunobiology. 219:687. PubMed
  81. Liang YH, et al. 2021. J Biomed Sci. 28:75. PubMed
  82. Shen C, et al. 2021. BMC Med. 19:283. PubMed
  83. Tsukamoto M, et al. 2019. Cancer Sci. 110:310. PubMed
  84. Li CY, et al. 2022. Int J Mol Sci. 23:. PubMed
  85. Wang H, et al. 2022. J Cancer. 13:2126. PubMed
  86. Kenty JH, et al. 2021. STAR Protocols. 2(3):100675. PubMed
  87. Zhang C, et al. 2020. Front Oncol. 0.944444444. PubMed
  88. Ye Y, et al. 2020. Genome Med. 0.557638889. PubMed
RRID
AB_940366 (BioLegend Cat. No. 329705)
AB_940368 (BioLegend Cat. No. 329706)

Antigen Details

Distribution

T cells, B cells, NK cells, monocytes/macrophages, granulocytes and dendritic cells

Function
CD274 is involved in the costimulatory signal, essential for T lymphocyte proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-CD1-independent manner. Its interaction with PD-1-CD1 inhibits T-cell proliferation and cytokine production.
Ligand/Receptor
PD-1 (PDCD1)
Cell Type
B cells, Dendritic cells, Fibroblasts, Granulocytes, Macrophages, Monocytes, NK cells, T cells
Biology Area
Cancer Biomarkers, Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Sharpe A, et al. 2007. Nat. Immunol. 8:239.

Gene ID
29126 View all products for this Gene ID
UniProt
View information about CD274 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All CD274 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC, IHC-P, Block
Biotin anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC
PE anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC
APC anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC
Brilliant Violet 421™ anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC
Ultra-LEAF™ Purified anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC, IHC, Block
PE/Cyanine7 anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC
Purified anti-human CD274 (B7-H1, PD-L1) (Maxpar® Ready) 29E.2A3 FC, CyTOF®
Brilliant Violet 711™ anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC
Brilliant Violet 605™ anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC
GoInVivo™ Purified anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC, IHC, Block
PE/Dazzle™ 594 anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC
Brilliant Violet 785™ anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC
Brilliant Violet 510™ anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC
PerCP/Cyanine5.5 anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC
Brilliant Violet 650™ anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC
Alexa Fluor® 594 anti-human CD274 (B7-H1, PD-L1) 29E.2A3 IHC-P
TotalSeq™-A0007 anti-human CD274 (B7-H1, PD-L1) 29E.2A3 PG
TotalSeq™-B0007 anti-human CD274 (B7-H1, PD-L1) 29E.2A3 PG
TotalSeq™-C0007 anti-human CD274 (B7-H1, PD-L1) 29E.2A3 PG
TotalSeq™-D0007 anti-human CD274 (B7-H1, PD-L1) 29E.2A3 PG
PE/Fire™ 810 anti-human CD274 (B7-H1, PD-L1) Antibody 29E.2A3 FC
PE/Cyanine5 anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC
Spark YG™ 570 anti-human CD274 (B7-H1, PD-L1) 29E.2A3 IHC-P, FC
Go To Top Version: 7    Revision Date: 11/30/2016

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD274 (B7-H1, PD-L1)

  • Biotin anti-human CD274 (B7-H1, PD-L1)

  • PE anti-human CD274 (B7-H1, PD-L1)

  • APC anti-human CD274 (B7-H1, PD-L1)

  • Brilliant Violet 421™ anti-human CD274 (B7-H1, PD-L1)

  • Ultra-LEAF™ Purified anti-human CD274 (B7-H1, PD-L1)

  • PE/Cyanine7 anti-human CD274 (B7-H1, PD-L1)

  • Purified anti-human CD274 (B7-H1, PD-L1) (Maxpar® Ready)

  • Brilliant Violet 711™ anti-human CD274 (B7-H1, PD-L1)

  • Brilliant Violet 605™ anti-human CD274 (B7-H1, PD-L1)

  • GoInVivo™ Purified anti-human CD274 (B7-H1, PD-L1)

  • PE/Dazzle™ 594 anti-human CD274 (B7-H1, PD-L1)

  • Brilliant Violet 785™ anti-human CD274 (B7-H1, PD-L1)

  • Brilliant Violet 510™ anti-human CD274 (B7-H1, PD-L1)

  • PerCP/Cyanine5.5 anti-human CD274 (B7-H1, PD-L1)

  • Brilliant Violet 650™ anti-human CD274 (B7-H1, PD-L1)

  • Alexa Fluor® 594 anti-human CD274 (B7-H1, PD-L1)

  • TotalSeq™-A0007 anti-human CD274 (B7-H1, PD-L1)

  • TotalSeq™-B0007 anti-human CD274 (B7-H1, PD-L1)

  • TotalSeq™-C0007 anti-human CD274 (B7-H1, PD-L1)

  • TotalSeq™-D0007 anti-human CD274 (B7-H1, PD-L1)

  • PE/Fire™ 810 anti-human CD274 (B7-H1, PD-L1) Antibody

  • PE/Cyanine5 anti-human CD274 (B7-H1, PD-L1)

  • Spark YG™ 570 anti-human CD274 (B7-H1, PD-L1)

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