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PE anti-human CD229 (Ly-9) Antibody

Pricing & Availability
Clone
HLy-9.1.25 (See other available formats)
Regulatory Status
RUO
Other Names
Ly9, Ly-9, SLAMF3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HLy-9dot1dot25_PE_011408
Human peripheral blood lymphocytes stained with HLy-9.1.25 PE
  • HLy-9dot1dot25_PE_011408
    Human peripheral blood lymphocytes stained with HLy-9.1.25 PE
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
326107 25 tests 108€
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326108 100 tests 234€
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Description

CD229 is a 100-120 kD glycoprotein. It is a member of the SLAM family, a CD2 subset of the Ig superfamily, known as Ly9 or SLAMF3. CD229 is expressed on T cells, B cells, NK cells, and thymocytes. It functions as a homophilic adhesion molecule through binding to CD229 itself. The cytoplasmic tail of CD229 binds to SAP and Grb2 proteins. CD229 is involved in enhancing T cell activation and Th2 polarization. HLy9.1.25 antibody recognizes epitope A in the first Ig domain of Ly9 molecule.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
300.19 cell line transfected with the CD229 full-length DNA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported (for the relevant formats) applications include: immunoprecipitation1,2, and immunocytochemistry3. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 326109 & 326110).

Application References
  1. de la Fuente MA, et al. 2001. Blood 97:3513.
  2. Martin M, et al. 2005. J. Immunol. 174:5977.
  3. Romero X, et al. 2005. J. Immunol. 174:7033.
RRID
AB_2137939 (BioLegend Cat. No. 326107)
AB_2137938 (BioLegend Cat. No. 326108)

Antigen Details

Structure
100-120 kD glycoprotein, SLAM family, CD2 subset of Ig superfamily
Distribution

T cells, B cells, NK cells, thymocytes

Function
Homophilic adhesion, T cell activation, Th2 cell polarization
Ligand/Receptor
CD229
Cell Type
B cells, NK cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Sandrin MS, et al. 1996. Immunogenetics 43:13.
2. de la Fuente MA, et al. 2001. Blood 97:3513.
3. Romero X, et al. 2005. J. Immunol. 174:7033.

Gene ID
4063 View all products for this Gene ID
UniProt
View information about CD229 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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