PE anti-Acetylated Lysine Antibody

Pricing & Availability
Clone
15G10 (See other available formats)
Regulatory Status
RUO
Other Names
Proteins are acetylated by the reversible transfer of acetyl-CoA to the ε-amino group of lysine
Isotype
Mouse IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
15G10_PE_acetylated_lysine_Antibody_FC_1_013114
Human cervical carcinoma cell line, HeLa, was incubated with (top) or without (bottom) Trichostatin A (100 ng/ml, overnight), then treated with FOXP3 Fix/Perm Buffer Set. Cells were then stained with anti-acetylated lysine (clone 15G10) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).
  • 15G10_PE_acetylated_lysine_Antibody_FC_1_013114
    Human cervical carcinoma cell line, HeLa, was incubated with (top) or without (bottom) Trichostatin A (100 ng/ml, overnight), then treated with FOXP3 Fix/Perm Buffer Set. Cells were then stained with anti-acetylated lysine (clone 15G10) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).
  • 15G10_PE_acetylated_lysine_Antibody_FC_2_013114
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
623404 100 tests 268€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Proteins are reversibly and dynamically acetylated on the ε-amino group of lysine by acetyltransferases and deacetylated by deacetylases. This post-translational modification can regulate protein function (interactions with other proteins and DNA binding). Histones and transcription factors (PCAF, p53, p300, etc) appear to be the major targets of acetyltransferases. Acetylation is usually associated with chromatin remodeling and transcriptional activation, although in some cases (telomeres) it is associated with gene silencing. The 15G10 antibody recognizes acetylated lysine residues on proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Species independent
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Acetylated protein mixture
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References
  1. Kuo HP, et al. 2010. Sci Signal. 3:Ra9. PubMed
Product Citations
  1. Fujii K, et al. 2021. Int J Mol Sci. 22:. PubMed
  2. Luque–Martin R, et al. 2019. Front Pharmacol. 1.279166667. PubMed
RRID
AB_2563126 (BioLegend Cat. No. 623404)

Antigen Details

Structure
Proteins are acetylated by the reversible transfer of acetyl-CoA to the ε-amino group of lysine
Function
Post-translational modification of proteins by acetyltransferases and deacetylated by deacetylases to regulate protein-protein and protein-DNA interactions
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics
Antigen References

1. Wang S, et al. 2004. Novartis Found. Symp. 259:238.
2. Legube G, et al. 2003. EMBO Rep. 4:944.
3. Chen LF, et al. 2003. J. Mol. Med. 81:549.

Gene ID
8850 View all products for this Gene ID
UniProt
View information about Acetylated Lysine on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 09/19/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account