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Pacific Blue™ anti-mouse F4/80 Antibody

Pricing & Availability
Clone
BM8 (See other available formats)
Regulatory Status
RUO
Other Names
EMR1, Ly71
Isotype
Rat IgG2a, κ
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Product Citations
publications
BM8_Blue_021808
Thioglycollate elicited Balb/c peritoneal macrophage cells stained with Pacific Blue™ BM8
  • BM8_Blue_021808
    Thioglycollate elicited Balb/c peritoneal macrophage cells stained with Pacific Blue™ BM8
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123123 25 µg 111€
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123124 100 µg 243€
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Description

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.

Application References
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Shah D, et al. 2021. Oncoimmunology. 10:1939601. PubMed
  2. Bucher K, et al. 2015. J Leukoc Biol. 2015;98: 365-372. PubMed
  3. Schneider C, et al. 2018. Cell. 174:271. PubMed
  4. Kwon O, et al. 2016. Stem Cell Res. 16: 682-691. PubMed
  5. Chen WS, et al. 2021. Cell Rep. 37:109974. PubMed
  6. Jard T, et al. 2020. Cell Stem Cell. 27(4):646-662.e7. PubMed
  7. Tanaka Y, et al. 2015. PLoS One. 10: 0138621. PubMed
  8. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  9. Ganeshan K et al. 2019. Cell. 177(2):399-413 . PubMed
  10. Murphy M, et al. 2017. Eur J Immunol. 47:880. PubMed
  11. Yang Y, et al. 2021. Nat Commun. 12:525. PubMed
  12. Dichtl S, et al. 2021. Sci Adv. 7: . PubMed
  13. Marangoni F, et al. 2021. Cell. . PubMed
  14. Philip N, et al. 2016. PLoS Pathog. 12:e1005910. PubMed
  15. Ni P, et al. 2014. J Immunol . 193:1778. PubMed
  16. Billi AC, et al. 2019. JCI Insight. 4:8. PubMed
  17. Syed A, et al. 2015. Infect Immun . 83: 3428-3437. PubMed
  18. Izquierdo-Useros N, et al. 2014. J Immunol . 192:4852-4858. PubMed
  19. Zhang B, et al. 2016. Mol Cell. 63: 976-89. PubMed
  20. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  21. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
  22. Saravia J, et al. 2015. PLoS Pathog. 11: e1005217. PubMed
  23. Goncalves R, et al. 2011. J Exp Med. 208:1253. PubMed
  24. Leonard JD et al. 2017. Immunity. 47(1):107-117 . PubMed
  25. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  26. Okamura T, et al. 2021. Front Immunol. 12:669629. PubMed
  27. Dar HH, et al. 2022. JCI Insight. 7:. PubMed
  28. Krishnamurthy B, et al. 2015. Diabetes. 64: 3229-3238. PubMed
  29. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  30. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  31. Cohen H, et al. 2015. J Immunol. 195: 3828 - 3837. PubMed
  32. Stolt C, et al. 2016. J Immunol. 197: 834 - 846. PubMed
  33. Boukelmoune N, et al. 2021. Brain Behav Immun. 93:43. PubMed
  34. Zhang X, et al. 2020. Endocr Relat Cancer. 27:469. PubMed
  35. Challa TD, et al. 2020. Cell Reports. 30(10):3424-3433. PubMed
  36. Kälin S et al. 2017. Cell metabolism. 26(3):475-492 . PubMed
  37. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  38. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  39. O'Leary CE, et al. 2021. Curr Protoc. 1:e77. PubMed
  40. Jiang L, et al. 2020. Cell. 183(5):1219-1233.e18. PubMed
  41. Draijer C, et al. 2018. Sci Rep. 8:5105. PubMed
  42. Jones M, et al. 2010. J Immunol. 185:3583. PubMed
  43. Flores AM, et al. 2020. Nat Nanotechnol. 0.731944444. PubMed
  44. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  45. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  46. Jee JJ, et al. 2022. Nat Commun. 13:18. PubMed
  47. Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
  48. Stack G, et al. 2015. PLoS Pathog. 11:1004641. PubMed
  49. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  50. Huanga J, et al. 2013. J Immunol Methods. 387:254. PubMed
  51. Fotakis P, et al. 2019. Arterioscler Thromb Vasc Biol. 39:e253. PubMed
RRID
AB_893487 (BioLegend Cat. No. 123123)
AB_893475 (BioLegend Cat. No. 123124)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 10/25/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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