- Clone
- UCHT1 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- III 471
- Other Names
- T3, CD3ε
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications

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Human peripheral blood lymphocytes stained with UCHT1 Pacific Blue™
CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
-
test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. - Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
- Concentration
- µg sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The CD3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
For test size, the suggested use of this reagent for immunofluorescent staining is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
For µg sizes, the suggested use of this reagent for immunofluorescent staining is ≤2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood.
It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
- Application References
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- Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
- Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
- Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
- Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
- Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
- Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
- Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
- Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
- Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
- Li J, et al. 2015. Cancer Res. 75:508. PubMed
- Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
- Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
-
- RRID
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AB_493095 (BioLegend Cat. No. 300418)
AB_1595437 (BioLegend Cat. No. 300431)
AB_493094 (BioLegend Cat. No. 300417)
AB_2562048 (BioLegend Cat. No. 300442)
Antigen Details
- Structure
- Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
- Distribution
-
Mature T and NK T cells, thymocyte differentiation
- Function
- Antigen recognition, signal transduction, T cell activation
- Ligand/Receptor
- Peptide antigen bound to MHC
- Cell Type
- NKT cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, TCRs
- Antigen References
-
1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507. - Gene ID
- 916 View all products for this Gene ID
- UniProt
- View information about CD3 on UniProt.org
Related FAQs
Other Formats
View All CD3 Reagents Request Custom ConjugationCustomers Also Purchased



Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD3
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Biotin anti-human CD3
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FITC anti-human CD3
-
PE anti-human CD3
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PE/Cyanine5 anti-human CD3
-
Purified anti-human CD3
-
Alexa Fluor® 647 anti-human CD3
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Alexa Fluor® 488 anti-human CD3
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Pacific Blue™ anti-human CD3
-
PE/Cyanine7 anti-human CD3
-
Alexa Fluor® 700 anti-human CD3
-
APC/Cyanine7 anti-human CD3
-
PerCP anti-human CD3
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PerCP/Cyanine5.5 anti-human CD3
-
Brilliant Violet 421™ anti-human CD3
-
Brilliant Violet 570™ anti-human CD3
-
Ultra-LEAF™ Purified anti-human CD3
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Purified anti-human CD3 (Maxpar® Ready)
-
Alexa Fluor® 594 anti-human CD3
-
PE/Dazzle™ 594 anti-human CD3
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Brilliant Violet 510™ anti-human CD3
-
Brilliant Violet 605™ anti-human CD3
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Brilliant Violet 711™ anti-human CD3
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Brilliant Violet 650™ anti-human CD3
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APC/Fire™ 750 anti-human CD3
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Pacific Blue™ anti-human CD3
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Brilliant Violet 785™ anti-human CD3
-
PE/Dazzle™ 594 anti-human CD3
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TotalSeq™-A0034 anti-human CD3
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TotalSeq™-B0034 anti-human CD3
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TotalSeq™-C0034 anti-human CD3
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PE anti-human CD3
-
PE/Cyanine7 anti-human CD3
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KIRAVIA Blue 520™ anti-human CD3
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Spark Violet™ 538 anti-human CD3 Antibody
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TotalSeq™-D0034 anti-human CD3
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Spark Blue™ 574 anti-human CD3 Antibody
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GMP Pacific Blue™ anti-human CD3
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GMP PE anti-human CD3
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GMP PE/Dazzle™ 594 anti-human CD3
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Spark Violet™ 423 anti-human CD3
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GMP PE/Cyanine7 anti-human CD3
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Spark Blue™ 515 anti-human CD3
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