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ELISA MAX™ Standard Set Mouse TNF-α

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Regulatory Status
RUO
Other Names
TNF-a ELISA Kit
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Product Citations
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mouse tnf-a_122109
  • mouse tnf-a_122109
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430901 5 plates 212€
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Description

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ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Mouse TNF-α ELISA MAX™ Capture Antibody (200X)
  • Mouse TNF-α ELISA MAX™ Detection Antibody (200X)
  • Mouse TNF-α Standard
  • Avidin-HRP (1000X)

Product Details

Verified Reactivity
Mouse
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application References
  1. Patole PS, et al. 2005. J. Am. Soc. Nephrol. 16:3273.
  2. Kawamura K,et al.2007.Biol Reprod.76:611.PubMed
  3. Tsujikawa K,et al.2007.Proc Natl Acad Sci USA.104:16702.PubMed
  4. Desy O, et al. 2010. Toxicol Sci. 117:303. PubMed
  5. Sing A, et al. 2011. Infect Immun. 79:4533. PubMed
  6. O'Meara TR, et al. 2013. MBio. 4:e00522. PubMed
  7. Pustelny C, et al. 2015. Infect Immun. 83:417. PubMed
  8. Cao YW, et al. 2015. J Ethnopharmacol. 161:92. PubMed
  9. Maruhashi T, et al. 2015. J Immunol. 194:5681.
Product Citations
  1. Pustelny C, et al. 2015. Infect Immun . 83:417. PubMed
  2. Oldenburg R, et al. 2018. Front Immunol. 9:2. PubMed
  3. Swee L, et al. 2016. Open Bio. 6: 160293. PubMed
  4. Bachran C, et al. 2017. Sci Rep.. 10.1038/s41598-017-17948-0. PubMed
  5. Poli V, et al. 2021. iScience. 24:103256. PubMed
  6. Guo Y, et al. 2020. J Clin Invest. 130:6301. PubMed
  7. Zhang W, et al. 2022. Front Pharmacol. 13:1031438. PubMed
  8. Nulty C, et al. 2021. Methods Mol Biol. 2283:175. PubMed
  9. Rother M, et al. 2018. Cell Host Microbe. 23:661. PubMed
  10. Lajqi T, et al. 2022. Biomedicines. 10:. PubMed
  11. Aryal P, et al. 2021. J Lipid Res. 62:100108. PubMed
  12. Renshaw H, et al. 2016. Infect Immun . 84: 1556 - 1564. PubMed
  13. Croasdell A, et al. 2016. J Immunol. 196: 2742 - 2752. PubMed
  14. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  15. Childers GM, et al. 2021. Environ Health Perspect. 129:47015. PubMed
  16. O'Meara T, et al. 2013. MBio. 4:e00522. PubMed
  17. Felgner S, et al. 2020. Elife. 9:00. PubMed
  18. Xu J, et al. 2020. J Am Soc Nephrol. 31:54:00. PubMed
  19. Hsieh PS, et al. 2021. Exp Ther Med. 188:21. PubMed
  20. Zhao Y, et al. 2018. Mol Cell. 69:505. PubMed
  21. Singh A, et al. 2011. Infect Immun. 79:4533. PubMed
  22. Fisher AL, et al. 2021. Nat Commun. 12:4026. PubMed
  23. Li F, et al. 2022. Cells. 11:. PubMed
  24. Li Y, et al. 2021. Cardiovasc Res. 890:117. PubMed
  25. Vargas-Muñiz J, et al. 2015. Fungal Genet Biol. 81: 41-51. PubMed
  26. Zhou B, et al. 2019. MBio. 10. PubMed
  27. Stravinskas Durigon T, et al. 2018. J Innate Immun. 10:279. PubMed
  28. Minamidate A, et al. 2021. Biochem Biophys Rep. 27:101065. PubMed
  29. Chen X, et al. 2021. J Extracell Vesicles. 10:e12069. PubMed
  30. Yang J, et al. 2017. Sci Rep. 7:42723. PubMed
  31. AM C, et al. 2015. Proc Natl Acad Sci U S A. 112 7557 . PubMed
  32. Jung S, et al. 2021. Endocrinology. 162(3):. PubMed
  33. Li HY, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:289. PubMed
  34. Barberi T, et al. 2012. PLoS One. 7:e29808. PubMed
  35. Tsujikawa K, et al. 2007. Proc Natl Acad Sci U S A. 104:16702. PubMed
  36. Chen X, et al. 2019. Mol Pharm. 2.534722222. PubMed
  37. Lajqi T, et al. 2021. Int J Mol Sci. 22:. PubMed
  38. Xiao L, et al. 2021. Oxid Med Cell Longev. 2021:6366254. PubMed
  39. Gu Y, et al. 2021. Front Immunol. 12:635267. PubMed
  40. Zhang J, et al. 2020. Pain. 161:1381. PubMed
  41. León-Cabrera S, et al. 2014. Int J Biol Sci. 9 948 . PubMed
  42. Ho DH, et al. 2018. Exp Neurobiol. 27:171. PubMed
  43. Liu Y, et al. 2015. BioMed Res Int. 2015 563425. PubMed
  44. Liu B, et al. 2021. Theranostics. 11:9311. PubMed
  45. Unsinger J, et al. 2021. J Leukoc Biol. 109:697. PubMed
  46. Raychowdhury R, et al. 2021. Immunohorizons. 5:818. PubMed
  47. Frost PS, et al. 2019. Cell Death Dis. 10:323. PubMed
  48. Pedicone C, et al. 2022. iScience. 25:104170. PubMed
  49. Li X, et al. 2016. J Leukoc Biol. 100: 581 - 588. PubMed
  50. Morzelle M, et al. 2016. PLoS One. 11:e0166123. PubMed
  51. Du H, et al. 2021. Life Sci Alliance. 4: . PubMed
  52. Zhang Z, et al. 2020. Front Immunol. 11:583276. PubMed
  53. Du H, et al. 2022. Brain Commun. 11:. PubMed
  54. Li L, et al. 2022. Stem Cell Res Ther. 13:479. PubMed
  55. Tang-Huau TL, et al. 2021. Viruses. 13: . PubMed
  56. Maruhashi T, et al. 2015. J Immunol. 194:5681. PubMed
  57. Désy O, et al. 2010. Toxicol Sci. 117:303. PubMed
  58. Reeves KM, et al. 2021. Clin Cancer Res. Online ahead of print. PubMed
  59. Cao Y, et al. 2015. J Ethnopharmacol. 161:92. PubMed
  60. Kawamura K, et al. 2007. Biol Reprod. 76:611. PubMed
  61. Wong MY, et al. 2020. J Neuroinflammation. 0.841666667. PubMed
  62. Xiong D, et al. 2019. Cell Rep. 28:804. PubMed
RRID
AB_2883995 (BioLegend Cat. No. 430901)
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • Coating Buffer: 8.4 g NaHCO3, 3.56 g Na2CO3, add DI H2O to 1.0 L, pH to 9.5 (BioLegend Cat. No. 421701 is recommended.)
  • Assay Diluent: 10% Fetal Bovine Serum or 1% BSA in Phosphate-Buffered Saline (PBS) (BioLegend Cat. No. 421203 is recommended.)
  • PBS: 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add DI water to 1.0 L, pH to 7.4
  • Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20 (BioLegend Cat. No. 421601 is recommended.)
  • Wash bottle or automated microplate washer
  • TMB Substrate Solution - BioLegend Cat. No. 421101 is recommended.
  • Stop Solution (2 N H2SO4)
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Absorbent paper

Antigen Details

Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
21926 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 09/11/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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