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MojoSort™ Mouse CD8 T Cell Isolation Kit

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Regulatory Status
RUO
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Product Citations
publications
MCD8TCellIsoKit_1_051215
A single cell suspension from pooled C57BL/6 mouse peripheral lymphoid tissues was prepared to isolate CD8+ T cells using the MojoSort™ Mouse CD8 T Cell Isolation Kit. Cells were stained with CD8 (clone 53-6.7) PE and CD3ε (clone 145-2C11) APC.
  • MCD8TCellIsoKit_1_051215
    A single cell suspension from pooled C57BL/6 mouse peripheral lymphoid tissues was prepared to isolate CD8+ T cells using the MojoSort™ Mouse CD8 T Cell Isolation Kit. Cells were stained with CD8 (clone 53-6.7) PE and CD3ε (clone 145-2C11) APC.
  • MCD8TCellIsoKit_2_051215
Cat # Size Price Quantity Check Availability Save
480007 10 tests 76€
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480008 100 tests 322€
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480035 200 tests 436€
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Description

Non CD8+ T cells are depleted by incubating your sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched CD8+ T cells are collected by decanting the liquid in a clean tube. These are your cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents

For Cat# 480007:

  • 100 µl Biotin-Antibody Cocktail
  • 100 µl Streptavidin Nanobeads

For Cat# 480008:

  • 1 ml Biotin-Antibody Cocktail
  • 1 ml Streptavidin Nanobeads

For Cat# 480035:

  • 2 vials of 1 ml Biotin-Antibody Cocktail each
  • 2 vials of 1 ml Streptavidin Nanobeads each

Product Details

Reactivity
Mouse
Formulation
Cocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide.
Preparation
The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Streptavidin Nanobeads: Streptavidin-coated magnetic beads.
Storage & Handling
Antibody cocktail and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested
Recommended Usage

10 µl of antibody cocktail for 1x107 cells in 100 µl of buffer.
10 µl Streptavidin Nanobeads for 1x107 cells in 100 µl of buffer.

Application Notes

This kit is designed for the isolation of untouched CD8+ T cells from lymphoid tissues.

Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
 

Antibody or cocktail dilution to use in column: 10X
Nanobead dilution to use in columns: 8X

Product Citations
  1. Dai K, et al. 2017. Front Immunol. 0.555555556. PubMed
  2. Aguilar–Valenzuela R, et al. 2018. J Virol. 92:e00014. PubMed
  3. Ma S, et al. 2018. Cell. 173:443. PubMed
  4. Nabe S, et al. 2018. Cancer Sci. 109:3737. PubMed
  5. Yan J, et al. 2019. Nat Commun. 0.727777778. PubMed
  6. Raju S, et al. 2020. Cell Reports. 29(9):2556-2564.e3.. PubMed
  7. Bai C, et al. 2020. Mol Ther Oncolytics. 17:9. PubMed
  8. Mosaheb MM, et al. 2020. Nat Commun. 11:524. PubMed
  9. Cheng Y, et al. 2020. PLoS Pathog. 16:e1008569. PubMed
  10. Ma C, et al. 2017. J Immunol. 198(2):749-756. PubMed
  11. Lucas ED, et al. 2020. Cell Reports. 33(2):108258. PubMed
  12. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  13. Lv M, et al. 2020. Cell Res. 30:966. PubMed
  14. Dai X, et al. 2020. Theranostics. 6.897222222. PubMed
  15. Li ZL, et al. 2020. Nat Commun. 3.101388889. PubMed

Antigen Details

Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
NA
UniProt
View information about CD8a on UniProt.org

Related FAQs

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Can your magnetic particles be sterile filtered?

Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

What antibodies are present in the depletion cocktails provided for isolation kits?

Please contact our technical service team for further assistance.

Go To Top Version: 5    Revision Date: 01/26/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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