LEGEND MAX™ Mouse GM-CSF ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
GM-CSF Pre-coated ELISA Kit
Ave. Rating
Submit a Review
Product Citations
publications
GM-CSF 061709
  • GM-CSF 061709
Cat # Size Price Quantity Check Availability Save
432207 1 Pre-coated Plate 276€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

GM-CSF (Granulocyte/Macrophage-colony stimulating factor, CSF-a, Pluripoietin-a, Eosinophil colony stimulating factor, Eo-CSF, Burst promoting activity, BPA) is a hematopoietic factor that is produced by activated T cells, B cells, mast cells, macrophages, fibroblasts and endothelial cells. In addition to supporting colony formation of granulocyte and macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte and eosinophil progenitors.

BioLegend’s LEGEND MAX™ Mouse GM-CSF ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Mouse GM-CSF specific monoclonal antibody is pre-coated on a 96-well strip-well plate.

BioLegend’s LEGEND MAX™ Mouse GM-CSF ELISA Kit is specifically designed for the accurate quantitation of Mouse GM-CSF from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Anti-Mouse GM-CSF Pre-coated 96-well Strip Microplate
  • Mouse GM-CSF Dectection Antibody
  • Mouse GM-CSF Standard
  • Matrix A (for serum and plasma samples only)
  • Avidin-HRP A
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Mouse
Application

ELISA

Application References
  1. Urdinguio RG,et al. 2013. Cancer Res. 79:395. PubMed.
Product Citations
  1. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  2. Scur M, et al. 2022. Nat Commun. 13:7272. PubMed
  3. Urdinguio R, et al. 2013. Cancer Res. 73:395. PubMed
Sensitivity
3.5 pg/mL
Standard Range
15.6-1,000 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
T cells, monocytes/macrophages, fibroblasts, endothelial cells, mast cells
Biology Area
Cell Biology, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Gene ID
12981 View all products for this Gene ID
UniProt
View information about GM-CSF on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 2    Revision Date: 09/11/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account