LEGEND MAX™ Free Active TGF-β1 ELISA Kit

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Other Names
Free Active TGF-ž1 Pre-coated ELISA Kit
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2 reviews
Product Citations
publications
TGF-b1_020711
  • TGF-b1_020711
Cat # Size Price Quantity Save
437707 1 Pre-coated Plate 340€
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Description

Transforming growth factor beta 1 (TGF-ß1) is a member of the transforming growth factor beta superfamily of cytokines.  TGF-ß1 precursor contains 390 amino acids with an N-terminal signal peptide of 29 amino acids required for secretion from a cell, a 249 amino acids pro-region ( latency associated peptide or LAP), and a 112 amino acids C-terminal region that becomes the active TGF-ß1 upon activation.

Both LAP and TGF-ß1 exist as homodimers in circulation, but the disulfide linked homodimers of LAP and TGF-ß1 remain non-covalently associated, forming the small latent TGF-ß1 complex (SLC, 100 kD).  The large latent TGF-ß1 Complex (LLC, 235 – 260 kD) contains a third component, the latent TGF-ß binding protein (LTBP), which is linked to LAP by a single disulfide bond.  The LTBP does not confer latency, but for efficient secretion of the complex to extracellular sites.  Free active TGF-ß1 can be released (activated) by many factors including enzymes and low or high pH. 

TGF-ß1 is nearly 100% conserved across mammalian species. It has diverse biological functions in multiple cellular processes such as regulating proliferation and differentiation of various cell types. TGF-ß1 is also an important immunoregulatory cytokine, which is involved in the maintenance of self-tolerance, Th17 differentiation, and T cell homeostasis etc.

It is expected that normal serum, plasma, or other biological fluid contains low concentration of free active TGF-ß1 and high concentration of Latent TGF-ß1. It is the free active form TGF-ß1 that binds TGF-ß receptor and exerts biological functions. However, it has been difficult to quantify the free active TGF-ß1 because of insufficient sensitivities of most assay products currently available on the market. It is necessary to measure both the free active form and total TGF-ß1 in biological samples to understand the TGF-ß1 functions.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Anti-TGF-β1 Pre-coated 96-well Strip microplate
  • TGF-β1 Detection Antibody
  • TGF-β1 Standard
  • Avidin-HRP D
  • Assay Buffer C
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Reactivity
Mouse, Human, Rat, Porcine, NHP
Application

ELISA

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Application References
  1. Tucker, TA., et al. 2011. Am J. Respir Cell Mol Biol. 46:173. PubMed
  2. Andreade BB, et al. 2013. J Infect Dis. 207:1379. PubMed
  3. Wen L, et al. 2014. PLoS One. 9:101841. PubMed
  4. Kemter AM, et al. 2015. Life Sci. 15:129. PubMed
Product Citations
  1. Tucker T, et al. 2012. Am J Respir Cell Mol Biol. 118:2784. PubMed
  2. Andrade B, et al. 2013. J Infect Dis. 207:1379. PubMed
  3. Wen L, et al. 2014. PLoS One. 9:101841. PubMed
  4. Kemter A, et al. 2015. Life Sci. 15:129. PubMed
  5. Smith C, et al. 2015. Cell Rep. 13:1137-48. PubMed
  6. Lefebvre J, et al. 2016. Sci Rep. 6: 25051. PubMed
  7. McInerney-Leo A, et al. 2016. J Med Genet. 53: 457 - 464. PubMed
  8. Lawson J, et al. 2016. Vet J. 214:1-6. PubMed
  9. Takeshima T, et al. 2016. Proc Natl Acad Sci U S A. 113: 11300 - 11305. PubMed
  10. Xu M, et al. 2018. Am J Transplant. 18:855. PubMed
Publication Library
Sensitivity
2.3 pg/mL
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
Many cell types, highly expressed on activated Tregs and platelets
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family
Cytokines/Chemokines, Growth Factors
Gene ID
21803 View all products for this Gene ID
UniProt
View information about TGF-beta1 on UniProt.org

Related FAQs

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

Dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The Wash buffer is the same for all the current LEGEND MAX™ kits. All the part numbers on the Wash Buffer bottles in these kits should be the same. For ELISA MAX™ Deluxe and ELISA MAX™ Standard sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

In your LEGEND MAX™ ELISA Kits, there is a step that calls for a washing of the plates before even adding any sample to it. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The washings were designed to remove these components before you start the assay. If you do not do the washings, the effect on assay performance is negligible.

Go To Top Version: 3    Revision Date: 10/11/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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