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- Clone
- N278/19 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- SYT3
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Western blot of HRP anti-Synaptotagmin-3 antibody (clone N278/19). Lane 1: Molecular weight marker; Lane 2: 20 μg of rat brain lysate; Lane 3: 20 μg of mouse brain lysate. The blots were incubated with 0.1 μg/mL of clone N278/19 at 4°C overnight. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 852603 | 25 µg | 95€ | ||||
| 852604 | 100 µg | 160€ | ||||
Synaptotagmin-3 (SYT3) is a member of the synaptotagmin family, which consists of 13 membrane trafficking proteins that contain a N-terminal transmembrane region, and two cytoplasmic C2 domains. Synaptotagmin-3 is mainly localized at the presynaptic terminals in the central nervous system, where it functions as a Ca2+ sensor and plays an important role in exocytosis of secretory vesicles and neurotransmitter release. It has been shown that Ca2+ influx triggers the interaction between the C2-domains of SYT3 and the phospholipid membranes.
Product DetailsProduct Details
- Verified Reactivity
- Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Fusion protein corresponding to amino acids 442-533 (cytoplasmic C2B domain) of mouse Synaptotagmin-3
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Not recommended to be used in WB with human brain lysate.
- RRID
-
AB_2750416 (BioLegend Cat. No. 852603)
AB_2750417 (BioLegend Cat. No. 852604)
Antigen Details
- Structure
- Synaptotagmine-3 is a 590 amino acid protein with a molecular mass of ~ 63 kD.
- Distribution
-
Tissue Distribution: Central Nervous System
Cellular Distribution: Endosome, plasma membrane, synaptic vesicles, and cytosol. - Function
- SYT3 functions as a Ca2+ sensor at the presynaptic terminals and plays a role in Ca2+-dependent exocytosis of secretory vesicles.
- Interaction
- SYT3 forms homodimers and heterodimers with SYT6, SYT9 and SYT10. It binds to SNARE-complex component syntaxin-1.
- Biology Area
- Cell Biology, Neuroscience, Synaptic Biology
- Molecular Family
- Presynaptic proteins
- Antigen References
-
- Saheki Y, et al. 2017. Adv Exp Med Biol. 997:83
- Wang YL, et al. 2017. Cell Mol Life Sci. 74(16):2917
- Gene ID
- 84258 View all products for this Gene ID
- UniProt
- View information about Synaptotagmin-3 on UniProt.org
Related FAQs
Other Formats
View All Synaptotagmin-3 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Synaptotagmin-3 | N278/19 | WB |
| HRP anti-Synaptotagmin-3 | N278/19 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Synaptotagmin-3
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HRP anti-Synaptotagmin-3
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