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- Clone
- N277/7 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Synaptotagmin-12, sytXII, synaptotagmin 12, Syt12, Synaptotagmin XII, Synaptotagmin-related gene 1 protein, Srg1, Sytr1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Western blot of HRP anti-Synaptotagmin-12 antibody. Lane 1: Molecular weight marker; Lane 2: 20 μg of human brain lysate; Lane 3: 10 μg of mouse brain lysate; Lane 4: 10 μg of rat brain lysate. The blots were incubated with 5 μg/mL of clone N277/7 or mouse IgG1 overnight at 4°C. The blot that was incubated with mouse IgG1 was incubated with HRP‐labeled goat anti‐mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 820403 | 25 µg | 95€ | ||||
| 820404 | 100 µg | 160€ | ||||
Synaptotagmin 12 (Syt12) is regulated by protein kinase A (PKA) and is unique from other synaptotagmins in that it does not bind to Ca2+. Syt12 interaction with Syt1 prevents SNARE complex binding. Expression of Syt12 induces spontaneous neurotransmitter release, providing a mechanism for spontaneous synaptic-vesicle exocytosis that is Ca2+-independent.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a fusion protein corresponding to amino acids 168-255 (cytoplasmic C2A domain) of mouse Synaptotagmin-12.
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2734583 (BioLegend Cat. No. 820403)
AB_2734584 (BioLegend Cat. No. 820404)
Antigen Details
- Structure
- Expected MW: 47 kD
- Biology Area
- Cell Biology, Neuroscience, Synaptic Biology
- Molecular Family
- Presynaptic proteins
- Gene ID
- 171180 View all products for this Gene ID
- UniProt
- View information about Synaptotagmin-12 on UniProt.org
Related FAQs
Other Formats
View All Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Synaptotagmin-12 | N277/7 | WB,IHC-P |
| HRP anti-Synaptotagmin-12 | N277/7 | WB |
| Alexa Fluor® 594 anti-Synaptotagmin-12 | N277/7 | IHC-P |
| Biotin anti-Synaptotagmin-12 | N277/7 | WB,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Synaptotagmin-12
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HRP anti-Synaptotagmin-12
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Alexa Fluor® 594 anti-Synaptotagmin-12
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Biotin anti-Synaptotagmin-12
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