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HRP anti-SOD1 Antibody

Pricing & Availability
Clone
O98B10 (See other available formats)
Regulatory Status
RUO
Other Names
Superoxide dismutase 1, Superoxide dismutase [Cu-Zn] , Superoxide Dismutase, Cystolic
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
O98B10_HRP_SOD1_Antibody_WB_050318.jpg
Western blot of HRP anti-SOD1 antibody (clone O98B10). Lane 1: Molecular weight marker; Lanes 2 & 3: 20 µg of human brain lysates; Lanes 4 & 5: 20 µg of rat brain lysates; Lanes 6 & 7: 20 µg of mouse brain lysates. The blot was incubated with 0.25 µg/mL of the primary antibody overnight at 4 °C. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • O98B10_HRP_SOD1_Antibody_WB_050318.jpg
    Western blot of HRP anti-SOD1 antibody (clone O98B10). Lane 1: Molecular weight marker; Lanes 2 & 3: 20 µg of human brain lysates; Lanes 4 & 5: 20 µg of rat brain lysates; Lanes 6 & 7: 20 µg of mouse brain lysates. The blot was incubated with 0.25 µg/mL of the primary antibody overnight at 4 °C. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
Cat # Size Price Quantity Check Availability Save
850703 25 µg 106€
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850704 100 µg 225€
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This product is scheduled for discontinuation. Please use the availability checker to determine current availability. Cat. Nos. 850701 & 850702 are available as an alternative.
Description

Superoxide dismutase 1 (SOD1) is one of three isozymes responsible for destroying free superoxide radicals in the body. SOD1 is a homodimeric cytoplasmic Cu/Zn binding protein that converts harmful superoxide radicals to oxygen and hydrogen peroxide. The other isozymes are SOD2 and SOD3, which are localized to the mitochondria and extracellular space, respectively. SOD1 mutations are associated with familial amyotrophic lateral sclerosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, Drosophila
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length human SOD1 expressed in 293T cells transfected with human SOD1 expression vector.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Reactivity to Drosophila was only verified with the purified format.

RRID
AB_2734630 (BioLegend Cat. No. 850703)
AB_2734631 (BioLegend Cat. No. 850704)

Antigen Details

Structure
SOD1 is a homodimeric β-barrel protein that contains an intramolecular disulfide bond and a binuclear Cu/Zn site in each subunit. SOD1 monomers have an apparent molecular weight of ~16 kD.
Distribution

Tissue sources: Ubiquitously expressed.  
Cellular distribution: Cytosol, mitochondrion, peroxisome and nucleus.

Function
SOD1 converts harmful superoxide radicals in the body to oxygen and hydrogen peroxide.
Interaction
UBC, CCS, PRDX1, HSPA4
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Enzymes and Regulators
Antigen References

1. Lee S, Kim HJ. 2015. Exp. Neurobiol. 24(1):1-7.
2. Tafuri F, et al. 2015. Front Cell Neurosci. 9: 336.
3. Palomo, GM, Manfredi G. 2015. Brain Res. 1607:36-46.
4. Rosen DR, et al. 1993. Nature 362 (6415):59-62.

Gene ID
6647 View all products for this Gene ID
UniProt
View information about SOD1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/22/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-SOD1

  • HRP anti-SOD1
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