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HRP anti-ADAM22 Antibody

Pricing & Availability
Clone
N46/30 (See other available formats)
Regulatory Status
RUO
Other Names
Disintegrin and metalloproteinase domain-containing protein 22, a disintegrin and metalloprotease domain (ADAM) 22, Metalloproteinase-disintegrin ADAM22-3, Metalloproteinase-like disintegrin-like and cysteine-rich protein 2, MDC2
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
1_N46slash30_HRP_ADAM22_Antibody_1_WB_052318
Western blot of HRP anti-ADAM22 antibody (clone N46/30). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain lysate; Lane 3: 20 µg of rat brain lysate. The blots were incubated with 1 µg/mL of clone N46/30 or mouse IgG2b overnight at 4°C. The blot that was incubated with mouse IgG2b was incubated with HRP‐labeled goat anti‐mouse IgG (Cat. No. 405306). HRP anti-β-tubulin antibody (clone AA10, Cat. No. 657409) was used as a loading control. Enhanced chemiluminescence was used as the detection system.
  • 1_N46slash30_HRP_ADAM22_Antibody_1_WB_052318
    Western blot of HRP anti-ADAM22 antibody (clone N46/30). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain lysate; Lane 3: 20 µg of rat brain lysate. The blots were incubated with 1 µg/mL of clone N46/30 or mouse IgG2b overnight at 4°C. The blot that was incubated with mouse IgG2b was incubated with HRP‐labeled goat anti‐mouse IgG (Cat. No. 405306). HRP anti-β-tubulin antibody (clone AA10, Cat. No. 657409) was used as a loading control. Enhanced chemiluminescence was used as the detection system.
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818403 25 µg 95€
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818404 100 µg 160€
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Description

ADAM22 is a member of the ADAM (a disintegrin and metalloprotease domain) family, implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Unlike other members of the ADAM protein family, the protein encoded by this gene lacks metalloprotease activity since it has no zinc-binding motif. The protein is highly expressed in the brain and may function as an integrin ligand in the brain. In mice, it has been shown to be essential for correct myelination in the peripheral nervous system.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 757-857 (cytoplasmic region) of mouse ADAM22 protein.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2734575 (BioLegend Cat. No. 818403)
AB_2734576 (BioLegend Cat. No. 818404)

Antigen Details

Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Postsynaptic proteins
Gene ID
11496 View all products for this Gene ID
UniProt
View information about ADAM22 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/06/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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