Go-ChIP-Grade™ Purified anti-STAT1 Phospho (Tyr701) Antibody

Pricing & Availability
Clone
A17012A (See other available formats)
Other Names
Signal Transduced Activator of Transcription 1, Transcription Factor ISGF-3 components p91/p84
Isotype
Mouse IgG1, κ
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Product Citations
publications
A17012A_PURE_STAT1_Antibody_0_WB_032918
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of HT1080 cells treated with IFNγ for 30 minutes with either A)1:50 dilution of Go-ChIP-Grade™ Purified anti-STAT1 Phospho (Tyr701) (Clone A17012A) or B) equal amount of Purified Mouse IgG1, κ Isotype Control Antibody (Clone MG1-45, Cat. No. 401401) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human TAP1 gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • A17012A_PURE_STAT1_Antibody_0_WB_032918
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of HT1080 cells treated with IFNγ for 30 minutes with either A)1:50 dilution of Go-ChIP-Grade™ Purified anti-STAT1 Phospho (Tyr701) (Clone A17012A) or B) equal amount of Purified Mouse IgG1, κ Isotype Control Antibody (Clone MG1-45, Cat. No. 401401) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human TAP1 gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • A17012A_PURE_STAT1_Antibody_1_WB_updated_020918
    Total cell lysates (15 µg protein) from serum-starved HeLa S3 cells treated without (Lane 1) or with (Lane 2) 10 ng/mL human IFN-α2 (Cat. No. 592702) for 10 minutes were resolved by 8% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 2 µg/mL (1:250 dilution) purified anti-STAT1 Phospho (Tyr701) antibody (clone A17012A). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse-IgG (Cat. No. 405301) at a 1:3000 dilution. Equal STAT1 loading was confirmed using a pan anti-STAT1 antibody that recognizes both STAT1 α (87 kD) and β (83 kD) isoforms.
  • A17012A_PURE_STAT1_Antibody_2_WB_updated_020918
    Total cell lysates (15 µg protein) from serum-starved HeLa S3 cells treated without (Lane 1) or with (Lane 2) 10 ng/mL human IFN-α2 (Cat. No. 592702) for 10 minutes were resolved by 8% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with the indicated concentration of purified anti-STAT1 Phospho (Tyr701) antibody (clone A17012A). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse-IgG (Cat. No. 405301) at a 1:3000 dilution. Equal protein loading was confirmed using Direct-Blot™ HRP anti-β-Actin antibody (Cat. No. 643807) used at a 1:5000 dilution.
  • A17012A_PURE_STAT1_Antibody_3_ICFC_020918
    Human peripheral blood mononuclear cells were treated with (left), or without (right) Recombinant Human Interferon-γ (Cat No. 570204) for 15 minutes, fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401) then stained with CD4 (clone A161A1) APC and purified anti-STAT1 Phospho (Tyr701) (clone A17012A), followed by anti-mouse IgG PE. Data was gated on lymphocyte and monocyte populations.
  • A17012A_PURE_STAT1_Antibody_4_ICFC_020918
    Human peripheral blood monocytes were treated with (filled histogram), or without (open histogram) Recombinant Human Interferon-γ (Cat. No. 570204) for 15 minutes, fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401) then stained with purified anti-STAT1 Phospho (Tyr701) (clone A17012A), followed by anti-mouse IgG PE.
  • A17012A_PURE_STAT1_Antibody_5_ICC_092418
    Serum starved HeLa cells were untreated (panel A) or stimulated with 100 ng/mL IFN-α2 (Cat. No. 592702, panel B) for 20 minutes, fixed with 4% paraformaldehyde for 10 minutes, permeabilized with ice-cold methanol for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a 1:100 dilution (5 µg/mL) of purified anti-STAT1 Phospho (Tyr701) Antibody, clone A17012A, for two hours at room temperature, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG Antibody(Cat. No. 405326) at 2.0 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
Cat # Size Price Quantity Avail. Save
666405 25 µg 76€
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666406 100 µg 180€
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Description

STAT1, also known as signal transduction and activator of transcription 1, is a ubiquitously expressed cytoplasmic protein and is activated in response to cytokine signaling, including IFN-α, IFN-γ, EGF, PDGF, and IL-6. Upon activation, STAT1 is phosphorylated at Tyrosine 701 (Tyr701) by receptor-associated kinases, including JAK1, JAK2, and TYK2. This results in STAT1 dimerization and subsequent translocation to the nucleus, where it functions as a transcriptional activator. STAT1 is involved in IFN-mediated immune responses, and STAT1-deficient mice are highly sensitive to bacterial and viral infections.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
WB, ICFC, ICC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50 by volume. For Western blotting, the suggested use of this reagent is 0.1 - 2.0 µg per ml. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration of 5.0 μg/ml is recommended. It is recommended that the reagent to be titrated for optimal performance before each experiment.

For intracellular flow cytometry our True-Nuclear™ Transcription Factor Staining Protocol is recommended.

Application Notes

Clone A17012A recognizes STAT1 phosphorylated at Tyrosine 701 (Tyr701).

When using this clone for ICC, we recommend using methanol to permeabilize fixed cells.

RRID
AB_2728501 (BioLegend Cat. No. 666405)
AB_2728502 (BioLegend Cat. No. 666406)

Antigen Details

Structure
Two isoforms of STAT1 exist as a result of alternative splicing. Isoform α is a 750 amino acid protein with a predicted molecular weight of 87 kD; the β isoform is a 712 amino acid protein with a predicted molecular weight of 83 kD.
Distribution

Ubiquitous tissue expression, nucleoplasmic-cytosolic distribution

Function
Immune response activation, cell signaling
Interaction
STAT1, STAT2, IRF9, ERBB4, JAK1, JAK2, TYK2, TNK1, SHP2
Biology Area
Cell Biology
Molecular Family
Nuclear Markers, Phospho-Proteins
Antigen References
  1. Moretti S, et al. 2017. J. Biol. Chem. 292: 1785.
  2. Wei J, et al. 2015. J. Immunol. 195: 2870.
  3. Sung PS, et al. 2015. Proc. Natl. Acad. Sci. USA. 112: 10443
  4. Ooi EL, et al. 2014. Proc. Natl. Acad. Sci. USA. 111: 1909.
  5. Wu TR, et al. 2002. J. Biol. Chem. 277: 47572.
  6. Horvath, et al. 1996. J. Virol. 70: 647.
  7. Haque SJ, et al. 1995. J. Biol. Chem. 270: 25709.
Gene ID
6772 View all products for this Gene ID
UniProt
View information about STAT1 Phospho (Tyr701) on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 03/30/2018

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