- Poly6226 (See other available formats)
- Other Names
- NFκB p65, nuclear factor kappa light chain enhancer in B cells p65
- Rabbit Polyclonal IgG
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- Product Citations
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NF-κB/p65 (nuclear factor kappa light chain enhancer in B cells p65) is a member of the Rel/dorsal family. This ubiquitously expressed nuclear protein is one subunit of the NF-kappa B complex consisting of a 65 kD transactivating subunit and a 50 kD DNA binding subunit. Both subunits are derived from larger precursor proteins. NF-κB acts as a transcriptional activator and was originally identified as an activator of kappa light chain in B cells. NF-κB/p65 is bound (with p50) to IκB inhibitor in cytoplasm in inactive form. Phosphorylation of IκB releases and NF-κB which goes to the nucleus to activate gene expression. NF-κB can be activated with LPS, TNF-α, phorbol ester, or IL-1. The Poly6226 antibody reacts with the C-terminal region of human, mouse, and rat NF-κB p65 and has been shown to be useful for Western blotting.Product Details
- Mouse, Rat, Human
- Antibody Type
- Host Species
- peptide-KLH, C-terminal
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% gelatin.
- The antibody was purified by antigen-affinity chromatography.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
ChIP - Quality tested
WB, ICC - Validated
- Recommended Usage
Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50 - 1:200 by volume. For Western blotting, the suggested use of this reagent is 0.05 µg per ml. For immunocytochemistry, the suggested use of the reagent is a dilution range of 1:100~1:400. It is recommended that the reagent to be titrated for optimal performance before each experiment.
- Application References
AB_2728468 (BioLegend Cat. No. 622603)
AB_2728469 (BioLegend Cat. No. 622604)
- Member of the Rel/dorsal family. One subunit of the NF-kappa B complex consisting of a 65 kD transactivating subunit and a 50 kD DNA binding subunit. Both subunits derived from larger precursor proteins
Ubiquitously expressed nuclear protein
- Transcriptional activator, originally identified as an activator of kappa light chain in B cells
- Interacts with NF-κB p50 and IκB
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
1. Baldwin AS, et al. 1996. Annu. Rev. Immunol. 14:649 (review).
2. Chen F, et al. 1999. Clin. Chem. 45:7 (review).
- Bound (with p50) to IkB inhibitor in cytoplasm in inactive form. Phosphorylation of IkB releases and NF-kB goes to the nucleus to activate gene expression. Stimulation with LPS, TNF-α, phorbol ester, or IL-1 activates
- Gene ID
- 4790 View all products for this Gene ID
- View information about NF-kB p65 on UniProt.org
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