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FITC Donkey anti-rabbit IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4064 (See other available formats)
Regulatory Status
RUO
Isotype
Donkey Polyclonal Ig
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Product Citations
publications
Poly4064_FITC_IgG_Antibody_IF_032613
Hela cells were stained with anti-GRASP65 (Poly6210) and secondarily labeled with FITC donkey anti-rabbit IgG (clone Poly4064).
  • Poly4064_FITC_IgG_Antibody_IF_032613
    Hela cells were stained with anti-GRASP65 (Poly6210) and secondarily labeled with FITC donkey anti-rabbit IgG (clone Poly4064).
Compare all formats See FITC spectral data
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406403 100 µg 67€
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Description

This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rabbit
Antibody Type
Polyclonal
Host Species
Donkey
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
FC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 2.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.

Product Citations
  1. Fisher E, et al. 2021. Front Immunol. 11:602254. PubMed
  2. Nicholas D, et al. 2015. Mol Cell Proteomics. 14:15. PubMed
  3. Jarosch S, et al. 2022. STAR Protoc. 3:101374. PubMed
  4. Zhao Y, et al. 2021. Front Immunol. 12:665442. PubMed
  5. Wong C, et al. 2014. J Leukoc Biol. 95:631. PubMed
  6. Sun Z, et al. 2021. eLife. 10:00. PubMed
  7. Callahan SM, et al. 2021. Curr Protoc. 1:e294. PubMed
  8. Tang H, et al. 2017. J Immunol. 199:570. PubMed
  9. Jarosch S, et al. 2021. Cell Rep Methods. 1:100104. PubMed
  10. Callahan S, et al. 2020. Cell Microbiol. 22:e13210. PubMed
  11. Woo YD, et al. 2020. J Allergy Clin Immunol. . PubMed
  12. Yang J, et al. 2020. Nature. 586:572. PubMed
  13. Kishore M et al. 2017. Immunity. 47(5):875-889 . PubMed
  14. Shen C, et al. 2021. Front Immunol. 12:680055. PubMed
  15. Cai Y, et al. 2019. Cell Rep. 27:3034. PubMed
  16. Niemeyer BF, et al. 2021. Nano Sel. . PubMed
  17. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  18. Yang Y, et al. 2020. J Clin Invest. 130:5508. PubMed
  19. Janczy J, et al. 2014. J Immunol. 193:5190. PubMed
  20. Daneshmandi S, et al. 2021. Cell Reports. 34(10):108831. PubMed
  21. Zeng G, et al. 2011. J Immunol. 187:190. PubMed
  22. Mohamadi F, et al. 2019. Biomed Rep. 10:183. PubMed
  23. Ramos-Ramírez P, et al. 2021. Front Immunol. 12:677550. PubMed
  24. Tittarelli A, et al. 2014. J Immunol. 192:1313. PubMed
  25. Che N, et al. 2021. Front Immunol. 12:626310. PubMed
  26. Liu F, et al. 2021. Front Microbiol. 12:692279. PubMed
  27. Ryzhov S, et al. 2012. J Pharmacol Exp Ther. 341:764. PubMed
RRID
AB_893531 (BioLegend Cat. No. 406403)

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Go To Top Version: 5    Revision Date: 03/16/2021

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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