FITC anti-mouse Ly-6G Antibody

Pricing & Availability
Clone
1A8 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus G
Isotype
Rat IgG2a, κ
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Product Citations
publications
1A8_FITC_082808.jpg
BALB/cmouse bone marrow cells stained with 1A8 FITC (gated on myeloid cells)
  • 1A8_FITC_082808.jpg
    BALB/cmouse bone marrow cells stained with 1A8 FITC (gated on myeloid cells)
See FITC spectral data
Cat # Size Price Quantity Avail. Save
127605 50 µg 60,00€
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127606 500 µg 200,00€
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Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for other applications.

Excitation Laser
Blue Laser (488 nm)
Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, and depletion4, 12-14. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).

Application References
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
Product Citations
  1. Buschor S, et al. 2017. PLoS Pathogens. 13(6):e1006476. PubMed
  2. Frieler RA, et al. 2017. Exp Neurol. 298:104. PubMed
  3. Wang L, et al. 2017. Front Immunol.. 10.3389/fimmu.2017.01754. PubMed
  4. Li Z, et al. 2018. Front Immunol. 9:347. PubMed
  5. Shevchenko MA, et al. 2018. J Immunol Res. 8:53. PubMed
  6. Nakamura K, et al. 2018. Cancer Cell. 33:634. PubMed
  7. Li Z et al. 2018. Immunity. 49(4):640-653 . PubMed
  8. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  9. Lee EKS, et al. 2018. Cell Host Microbe. 23:121. PubMed
  10. Robinett RA et al. 2018. Cell systems. 7(1):41-48 . PubMed
  11. Huixin Xu et al. 2018. EMBO molecular medicine. 10(9) pii: e8931. PubMed
  12. Nagatake T, et al. 2018. Int Immunol. 30:471. PubMed
  13. Dyer DP et al. 2019. Immunity. 50(2):378-389 . PubMed
  14. Orr MT, et al. 2019. NPJ Vaccines. 4:1. PubMed
  15. Geng S, et al. 2019. Sci Adv. 5:eaav2309. PubMed
  16. Hermetet F, et al. 2019. Nat Commun. 10:523. PubMed
  17. Thomas DG et al. 2018. Cell reports. 25(13):3774-3785 . PubMed
  18. Uderhardt S, et al. 2019. Cell. 177:541. PubMed
  19. Billi AC, et al. 2019. JCI Insight. 4:8. PubMed
  20. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  21. He J, et al. 2019. J Clin Invest. 130. PubMed
  22. Christ A, et al. 2018. Cell. 172:162. PubMed
  23. Lai SM et al. 2018. Cell reports. 25(11):3099-3109 . PubMed
  24. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  25. Bradley KC, et al. 2019. Cell Rep. 28:245. PubMed
  26. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  27. Li YT, et al. 2019. Elife. 8:e47642. PubMed
  28. Patsalos A, et al. 2019. J Immunol. 203:1532. PubMed
  29. Roy K, et al. 2019. Immunity. 50:616. PubMed
  30. Yu L, et al. 2019. Elife. 8:e46677. PubMed
  31. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  32. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
  33. Sparber F, et al. 2019. Cell Host Microbe. 25:389. PubMed
  34. Luan HH et al. 2019. Cell. 178(5):1231-1244 . PubMed
  35. Chen ST et al. 2019. Cell host & microbe. 25(4):602-616 . PubMed
  36. Rebecca S Dickinson et al. 2017. Wellcome open research. 0.155555556 . PubMed
  37. Pan W et al. 2017. Immunity. 47(2):284-297 . PubMed
  38. Wang H et al. 2017. Developmental cell. 40(6):566-582 . PubMed
  39. Deng Z, et al. 2017. Oncogene. 36:639. PubMed
  40. Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed
  41. Lagares D, et al. 2017. Sci Transl Med. 9:eaal3765. PubMed
  42. Xiao P, et al. 2019. J Exp Med. 216:337. PubMed
  43. Nguyen CM, et al. 2019. Diabetes. 68:1499. PubMed
  44. Patel S, et al. 2019. Viruses. 0.877083333. PubMed
  45. Tomida S, et al. 2019. Sci Rep. 9:10751. PubMed
  46. Liu YG, et al. 2019. FASEB J. 33:5018. PubMed
  47. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  48. Saika A, et al. 2020. FASEB Bioadv. 2:59. PubMed
  49. Huai W, et al. 2019. J Exp Med. 216:772. PubMed
  50. Jin RM, et al. 2017. J Immunol. 198:352. PubMed
  51. Ueki H, et al. 2020. Nat Protoc. 15:1041. PubMed
  52. Winchell CG, et al. 2020. Front Immunol. 1.077083333. PubMed
  53. Challa TD, et al. 2020. Cell Reports. 30(10):3424-3433. PubMed
  54. Kotfis K, et al. 2020. British Journal of Pharmacology. 49(1):66-72. PubMed
  55. Gordan S, et al. 2020. Cell Reports. 29(10):3033-3046.e4.. PubMed
  56. Lin R, et al. 2020. Sci Rep. 10:14397. PubMed
  57. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  58. Jain A, et al. 2020. Nat Immunol. 0.920138889. PubMed
  59. Willemze RA, et al. 2019. Mol Med. 25:01:00. PubMed
  60. Fischer KJ, et al. 2019. J Immunol. 202:2027. PubMed
  61. Simpson K, et al. 2012. J Immunol. 189:5533. PubMed
  62. Fujikura D, et al. 2013. PLoS One. 8:e55321. PubMed
  63. Wu M, et al. 2013. Proc Natl Acad Sci U S A. 110:9439. PubMed
  64. Jacome-Galarza C, et al. 2011. J Bone Miner Res. 26:1207. PubMed
  65. Gorina R, et al. 2014. J Immunol. 192:324. PubMed
  66. Abad C, et al. 2014. J Leukoc Biol. 95:357. PubMed
  67. Weinheimer-Haus E, et al. 2014. PLoS One. 9:91355. PubMed
  68. Xu Y, et al. 2014. Proc Natl Acad Sci U S A. 111:6371. PubMed
  69. Mandraju R, et al. 2014. J Immunol. 192:4303. PubMed
  70. Minogue A, et al. 2014. Neurobiol Aging. 35:1442. PubMed
  71. Chen J, et al. 2014. Cell Res. 24:1050. PubMed
  72. Ericson J, et al. 2014. PLoS One. 9:108553. PubMed
  73. Nacer A, et al. 2014. PLoS Pathog. 10:1004528. PubMed
  74. Castiglioni A, et al. 2015. PLoS One. 10:128094. PubMed
  75. Shih Y, et al. 2015. JPEN J Parenter Enteral Nutr. . PubMed
  76. Kim P, et al. 2015. Infect Immun . 83: 2992 - 3002. PubMed
  77. Oliveira-Lima OC, et al. 2015. Immunobiology. . PubMed
  78. Clark K, et al. 2015. J Autoimmun. Available online 21 August 2015. PubMed
  79. Stadtmann A, et al. 2015. J Immunol. 195: 1152-1161. PubMed
  80. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  81. Syed A, et al. 2015. Infect Immun . 83: 3428-3437. PubMed
  82. Wang C, et al. 2015. Sci Rep. 5: 14124. PubMed
  83. Radjabova V, et al. 2015. J Immunol. 195: 3149 - 3159. PubMed
  84. Guesdon W, et al. 2015. J Infect Dis. 212: 1332 - 1340. PubMed
  85. Liu Z, et al. 2016. J Biol Chem. 291: 1441 - 1447. PubMed
  86. Montufar-Solis D, Klein J 2016. PLoS One. 11: 014611. PubMed
  87. Gousopoulos E, et al. 2016. Am J Pathol. 186:2193-2203. PubMed
  88. Cojohari O, Peppenelli M, Chan G 2016. Blood. 128: 529 - 541. PubMed
  89. Jia H, et al. 2016. J Immunol. 197: 859 - 871. PubMed
  90. Singh N, et al. 2016. Dis Model Mech. 9: 885 - 897. PubMed
  91. Diao N, et al. 2016. Sci Rep. 6:34672. PubMed
  92. Torre S, et al. 2016. Nat Immunol. 10.1038/ni.3581. PubMed
  93. Valladao A, et al. 2016. J Immunol. 197(12):4541-4551. PubMed
  94. Campbell IK, Leong D 2016. J Immunol. 197(11):4392-4402. PubMed
  95. Gamradt P, et al. 2016. PLoS Pathog. 12:e1006032. PubMed
  96. Strother R, et al. 2016. J Immunol. 197(11):4301-4311. PubMed
  97. Long M, et al. 2017. J Immunol. 198(2):862-872. PubMed
  98. Rao S, et al. 2017. Cell. 168(3):503-516.e12. PubMed
  99. Xu G, et al. 2020. Cell Rep. 32:108170. PubMed
  100. Varikuti S, et al. 2020. Br J Cancer. 122:1005. PubMed
  101. Danzer H, et al. 2020. Elife. 9:00. PubMed
  102. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  103. Elahi S, et al. 2020. Stem Cell Res. 43:101710. PubMed
  104. Taya M, et al. 2020. Endocr Relat Cancer. 27:261. PubMed
  105. Miller CM, et al. 2020. J Virol. 94:00:00. PubMed
  106. Garcia-Fabiani MB, et al. 2020. Methods Enzymol. 632:369. PubMed
  107. Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed
  108. Xu C, et al. 2020. Immunity. 53(2):417-428.e4. PubMed
  109. Kim S, et al. 2020. Immunity. 53(4):759-774.e9. PubMed
  110. Asadi Shahmirzadi A, et al. 2020. Cell Metabolism. 32(3):447-456.e6. PubMed
  111. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
  112. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  113. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  114. Ratitong B, et al. 2021. Cell Reports. 35(7):109139. PubMed
  115. Giampazolias E, et al. 2021. Cell. . PubMed
RRID
AB_1236488 (BioLegend Cat. No. 127605)
AB_1236494 (BioLegend Cat. No. 127606)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 01/06/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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